Adaptation of a silica membrane-based kit for purification of sequencing-ready BAC DNA

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dc.contributor.authorSakiyama, Ney S.
dc.contributor.authorVallejos, C. Eduardo
dc.contributor.authorJones, Valerie
dc.contributor.authorShanker, Savita
dc.date.accessioned2018-11-28T18:41:32Z
dc.date.available2018-11-28T18:41:32Z
dc.date.issued2007-07-25
dc.description.abstractWe describe a modification of a protocol for the isolation of BAC DNA using a silica membrane-based kit designed for the isolation of plasmid DNA. The major advantages of this protocol are the expediency of the procedure, the high yield and purity, and the high quality of the BAC DNA that is suitable for direct sequencing.en
dc.formatpdfpt-BR
dc.identifier.issn15729818
dc.identifier.urihttp://dx.doi.org/10.1007/s11105-007-0001-8
dc.identifier.urihttp://www.locus.ufv.br/handle/123456789/22624
dc.language.isoengpt-BR
dc.publisherPlant Molecular Biology Reporterpt-BR
dc.relation.ispartofseriesVolume 25, Issue 1– 2, Pages 11– 16, June 2007pt-BR
dc.rightsSpringer-Verlag 2007pt-BR
dc.subjectBAC DNA purificationpt-BR
dc.subjectDirect BAC DNA sequencingpt-BR
dc.subjectBAC fingerprintingpt-BR
dc.titleAdaptation of a silica membrane-based kit for purification of sequencing-ready BAC DNAen
dc.typeArtigopt-BR

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