Introgression of resistance to pathogens in common bean lines with the aid of molecular markers

Abstract

Aiming to incorporate resistance to the anthracnose and angular leaf spot pathogens in the common bean lines BRSMG Talismã, VC8 and VC9, crosses were made between these lines and the line Rudá-R, donor of the alleles Phg1, Co-4, Co-10, which confer resistance to Pseudocercospora griseola and Colletotrichum lindemuthianum. After the crosses, the backcross populations were obtained, and the RC1F1 plants were inoculated with race 65 of C. lindemuthianum. The resistant plants were genotyped with the markers SCARH13, SCARY20 and SCARF10, linked to the alleles Phg1, Co-4 and Co-10, respectively. Based on the molecular data, 44 plants were selected. The progenies originating from multiplication of these plants were evaluated over three seasons for grain yield, plant architecture and grain aspects. Based on these considerations and molecular data, 13 promising progenies were selected for developing inbreds.

Visando incorporar resistência aos patógenos da antracnose e da mancha-angular nas linhagens VC8 e VC9 e na cultivar BRSMG Talismã, foram realizados cruzamentos destas com a linhagem Rudá-R, doadora dos alelos Phg1, Co-4, Co-10, que conferem resistência à Pseudocercospora griseola e Colletotrichum lindemuthianum. Após a realização dos cruzamentos e obtenção das populações de retrocruzamentos, as plantas RC1F1 foram inoculadas com a raça 65 de C. lindemuthianum. As resistentes foram genotipadas com os marcadores SCARH13, SCARY20 e SCARF10, ligados aos alelos Phg1, Co-4 e Co-10, respectivamente. Com base nos dados moleculares foram selecionadas 44 plantas. As progênies provenientes da multiplicação destas plantas foram avaliadas em três safras quanto à produtividade de grãos, arquitetura de planta e aspecto dos grãos. Levando-se em conta estas avaliações e os dados moleculares, foram selecionadas 13 progênies promissoras para a extração de linhagens.