Evaluation of some oxidative-stress and antioxidant markers in goats during estrous cycle under Egyptian environmental conditions

Abstract

The objective of this study was to explore the impact of thermal stress on hormonal level and antioxidant activity in goats during various phases of the estrous cycle in the Egyptian summer. Forty cycling does were allocated to two groups (20 animals each) divided by season (mild/hot). Daily meteorological, rectal temperature, and respiratory rate data were recorded in the two seasons. The estrous cycle of the goats was synchronized by two intramuscular injections of 5 mg of prostaglandin F2α (PGF2α) at 11-day intervals. Blood samples were collected every two days and the levels of total antioxidant, malondialdehyde, and β-carotene were estimated. Total protein, albumin, cholesterol, triglycerides, progesterone, and thyroxine (T4 ) hormones were additionally measured in the serum of the collected samples. Under hot circumstances, both rectal temperature and respiratory rate increased considerably, with significant variation during the different stages of the estrous cycle. On the other hand, serum level of total protein, albumin, cholesterol, and triglycerides reduced significantly in animals in the hot environment, particularly during the luteal period of the estrous cycle (IV). During the Egyptian hot summer, the serum level of progesterone and T4 hormones declined in phases II and IV of the estrous cycle for progesterone and T4 , respectively. Moreover, the serum content of all oxidative stress markers tested (total antioxidant, β-carotene, and malondialdehyde) decreased considerably, especially in estrous cycle phase II in the hot environment. The Egyptian environmental conditions have detrimental effects on some antioxidant agents and some biochemical parameters throughout the estrous cycle of goats