Fractionation of carbohydrates and protein and rumen degradation kinetic parameters of brachiaria grass silage enriched with rice bran

Abstract

This experiment was conducted to evaluate the protein and carbohydrate fractions, and to estimate the in situ rumen degradation kinetic parameters of Brachiaria decumbens grass silage with inclusion of rice bran. Five rice bran inclusion levels were tested: 0, 10, 20, 30 and 40% of the natural matter, distributed into a completely randomized design with five replications. The brachiaria grass was obtained at 60 days of regrowth by cutting the forage at 10 cm from the soil and ensiling it in 10-L experimental silo bags, which were opened after 40 days. The degradability profiles for DM, CP, NDF and of the feeds for each animal utilized made it possible to obtain the estimates of the parameters analyzed. The contents of total carbohydrates (TCH), fibrous carbohydrates and fraction A+B1 of the TCH decreased linearly as the rice bran levels were elevated. Fraction A of the protein was increased linearly by 0.64% for every 1% of rice bran added to the silage. For nitrogenous fractions B3 and C, there was a decrease of 0.11 and 0.40% for each 1% of rice bran added to the silage, respectively. For the rumen degradability parameters of the dry matter, fractions A and I had estimated increases of 0.54 and 0.04% for every 1% inclusion of rice bran. For the degradation rate of fraction B (c), treatments had no effect. Fractions A and I of the crude protein degradability parameters increased by 0.22 and 0.72%, respectively, with inclusion of 1% rice bran. Inclusion levels of rice bran had no effect on fraction D, on the degradation rate of fraction B (c) or lag time (L) estimated for neutral detergent fiber. Inclusion of 10 and 20% rice bran in the brachiaria grass silage improves protein and carbohydrate fractions and rumen degradation kinetic parameters of dry matter, crude protein and neutral detergent fiber.