Spodoptera frugiperda versus isocicloseram: efeitos letais e subletais em diferentes instares
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Universidade Federal de Viçosa
Abstract
Recentemente introduzido no Brasil, o isocicloseram é um inseticida sintético da classe das isoxazolinas com novo modo de ação. Apresenta amplo espectro de ação contra diversos insetos-praga; entretanto, informações sobre sua eficácia frente à lagarta-do-cartucho, Spodoptera frugiperda (Lepidoptera: Noctuidae), ainda são limitadas, com relatos de ineficácia do inseticida em condições de campo. Neste estudo, avaliaram-se os efeitos letais e subletais do isocicloseram em diferentes ínstares de S. frugiperda. A alta suscetibilidade de larvas de 1º ínstar foi evidenciada pela baixa LC50 (0,068 mg/L) em relação à dose de campo recomendada (800 mg/L), indicando a eficácia do composto no controle de ínstares iniciais. Mesmo em concentrações 125 vezes menores que a concentração de campo, a exposição ao inseticida foi associada à redução de até 63% na massa larval e 28% na massa pupal. Na geração filial (F1), a massa larval foi reduzida em 47% numa concentração 3125 vezes menor que a concentração de campo. Embora o tempo de desenvolvimento não tenha sido alterado na geração parental (P0), a geração F1 apresentou reduções discretas, de 3,49% e 8,06%, indicando possíveis ajustes fisiológicos decorrentes da exposição parental. O índice de desempenho biológico (IDBio) foi significativamente reduzido sob exposição direta, com queda de até 76% nas maiores concentrações, enquanto insetos expostos no 1º ínstar apresentaram redução de aproximadamente 50%. Em contraste, na geração F1 oriunda de larvas expostas no 5º ínstar, observou-se aumento do IDBio em baixas concentrações (6,25% e 13,75%), sugerindo uma resposta hormética. Considerando que os efeitos ocorreram em concentrações muito inferiores às de uso em campo (800 mg/L), o isocicloseram mostra-se como um potente lagarticida e pode contribuir para o controle de S. frugiperda. Palavras-chave: Lagarta do cartucho, isocicloseram, isoxazolinas, efeitos subletais, hormese.
Recently introduced in Brazil, isocycloseram is a synthetic insecticide belonging to the isoxazoline class, with a novel mode of action. It exhibits a broad spectrum of activity against several insect pests; however, information regarding its efficacy against the fall armyworm, Spodoptera frugiperda (Lepidoptera: Noctuidae), remains limited, with reports of reduced effectiveness under field conditions. In this study, the lethal and sublethal effects of isocycloseram were evaluated across different instars of S. frugiperda. The high susceptibility of first-instar larvae was demonstrated by the low LC value (0.068 mg/L) relative to the recommended field dose (800 mg/L), indicating the compound’s effectiveness for controlling early instars. Even at concentrations 125-fold lower than the field rate, insecticide exposure resulted in reductions of up to 63% in larval mass and 28% in pupal mass. In the filial generation (F1), larval mass decreased by 47% at a concentration 3,125-fold lower than the field rate. Although developmental time was not affected in the parental generation (P0), the F1 generation exhibited slight reductions (3.49% and 8.06%), suggesting possible physiological adjustments resulting from parental exposure. The biological performance index (IDBio) was significantly reduced under direct exposure, with decreases of up to 76% at higher concentrations, while insects exposed at the first instar showed an approximate 50% reduction. In contrast, the F1 generation derived from larvae exposed at the fifth instar exhibited increases in IDBio at low concentrations (6.25% and 13.75%), suggesting a hormetic response. Considering that these effects occurred at concentrations far below that recommended as the field rate (800 mg/L), isocycloseram can be considered a potent insecticide with potential to contribute to the control of S. frugiperda. Keywords: Fall armyworm, isocycloseram, isoxazolins, sublethal effects, hormesis.
Recently introduced in Brazil, isocycloseram is a synthetic insecticide belonging to the isoxazoline class, with a novel mode of action. It exhibits a broad spectrum of activity against several insect pests; however, information regarding its efficacy against the fall armyworm, Spodoptera frugiperda (Lepidoptera: Noctuidae), remains limited, with reports of reduced effectiveness under field conditions. In this study, the lethal and sublethal effects of isocycloseram were evaluated across different instars of S. frugiperda. The high susceptibility of first-instar larvae was demonstrated by the low LC value (0.068 mg/L) relative to the recommended field dose (800 mg/L), indicating the compound’s effectiveness for controlling early instars. Even at concentrations 125-fold lower than the field rate, insecticide exposure resulted in reductions of up to 63% in larval mass and 28% in pupal mass. In the filial generation (F1), larval mass decreased by 47% at a concentration 3,125-fold lower than the field rate. Although developmental time was not affected in the parental generation (P0), the F1 generation exhibited slight reductions (3.49% and 8.06%), suggesting possible physiological adjustments resulting from parental exposure. The biological performance index (IDBio) was significantly reduced under direct exposure, with decreases of up to 76% at higher concentrations, while insects exposed at the first instar showed an approximate 50% reduction. In contrast, the F1 generation derived from larvae exposed at the fifth instar exhibited increases in IDBio at low concentrations (6.25% and 13.75%), suggesting a hormetic response. Considering that these effects occurred at concentrations far below that recommended as the field rate (800 mg/L), isocycloseram can be considered a potent insecticide with potential to contribute to the control of S. frugiperda. Keywords: Fall armyworm, isocycloseram, isoxazolins, sublethal effects, hormesis.
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SANTOS, Amanda de Souza. Spodoptera frugiperda versus isocicloseram: efeitos letais e subletais em diferentes instares. 2026. 35 f. Dissertação (Mestrado em Entomologia) - Universidade Federal de Viçosa, Viçosa. 2026.
