Fitopatologia - Artigos

URI permanente para esta coleçãohttps://locus.ufv.br/handle/123456789/11741

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    Etiology of bacterial leaf blight of eucalyptus in Brazil
    (Tropical Plant Pathology, 2008-05) Oliveira, José R.; Maffia, Luiz A.; Alfenas, Acelino C.; Gonçalves, Rivadalve C.; Lau, Douglas; Cascardo, Júlio C. M.
    Bacterial leaf blight of eucalyptus is initially characterized by water soaked, angular, amphigenous and interveinal lesions, concentrated along the main vein, at the edges or scattered on the leaf blade. As the disease progresses, the lesions become brown to pale, and when young leaves are infected leaf cut areas at the edges or perforations at the center of the lesions may appear due to abortion of the necrotic area. Eventually, necrosis may be found on petiole and twigs. Leaf fall commonly occurs on highly susceptible genotypes due to the early senescence of diseased leaves. Precise diagnosis is accomplished by bacterial exudation from leaf sections placed in a water drop under light microscope (200 x). Twenty-five bacterial isolates from Amapá (2), Bahia (4), Minas Gerais (2), São Paulo (9), Pará (3), Mato Grosso do Sul (1), and Rio Grande do Sul (4) States, which induced hypersensitive reaction (HR) in non-host plants and were pathogenic to eucalyptus, when inoculated by inoculum injection, were identified by biochemical assays, using carbon sources (MicroLogTM BIOLOG) and sequence analysis (16S rDNA). Ten isolates were identified as Xanthomonas axonopodis, four as X. campestris, four as Pseudomonas syringae, two as P. putida, two as P. cichorii, one as Erwinia sp., and two were similar to bacterial genera of Rhizobiaceae. When spray inoculated on intact plants of eucalyptus, only X. axonopodis, P. cichorii and isolates of the Rhizobiaceae family induced typical symptoms of the disease and were considered pathogenic. In Brazil, X. axonopodis seems to be the most widespread species causing the bacterial leaf blight of Eucalyptus spp.
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    Etiology of bacterial leaf blight of eucalyptus in Brazil
    (Tropical Plant Pathology, 2008-05) Oliveira, José R.; Maffia, Luiz A.; Cascardo, Júlio C. M.; Alfenas, Acelino C.; Gonçalves, Rivadalve C.; Lau, Douglas
    Bacterial leaf blight of eucalyptus is initially characterized by water soaked, angular, amphigenous and interveinal lesions, concentrated along the main vein, at the edges or scattered on the leaf blade. As the disease progresses, the lesions become brown to pale, and when young leaves are infected leaf cut areas at the edges or perforations at the center of the lesions may appear due to abortion of the necrotic area. Eventually, necrosis may be found on petiole and twigs. Leaf fall commonly occurs on highly susceptible genotypes due to the early senescence of diseased leaves. Precise diagnosis is accomplished by bacterial exudation from leaf sections placed in a water drop under light microscope (200 x). Twenty-five bacterial isolates from Amapá (2), Bahia (4), Minas Gerais (2), São Paulo (9), Pará (3), Mato Grosso do Sul (1), and Rio Grande do Sul (4) States, which induced hypersensitive reaction (HR) in non-host plants and were pathogenic to eucalyptus, when inoculated by inoculum injection, were identified by biochemical assays, using carbon sources (MicroLogTM BIOLOG) and sequence analysis (16S rDNA). Ten isolates were identified as Xanthomonas axonopodis, four as X. campestris, four as Pseudomonas syringae, two as P. putida, two as P. cichorii, one as Erwinia sp., and two were similar to bacterial genera of Rhizobiaceae. When spray inoculated on intact plants of eucalyptus, only X. axonopodis, P. cichorii and isolates of the Rhizobiaceae family induced typical symptoms of the disease and were considered pathogenic. In Brazil, X. axonopodis seems to be the most widespread species causing the bacterial leaf blight of Eucalyptus spp.
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    Variáveis climáticas associadas à incidência de mofo-cinzento em eucalipto
    (Fitopatologia Brasileira, 2006-03) Mafia, Reginaldo G.; Alfenas, Acelino C.; Ferreira, Eraclides M.; Leite, Fernando Palha; Souza, Flávio Luiz de
    Com base em dados de infecção natural avaliou-se a incidência do mofo-cinzento, causado por Botrytis cinerea, em 14 clones de Eucalyptus spp. em relação às condições climáticas predominantes em um viveiro clonal localizado em Belo Oriente, Minas Gerais. A temperatura máxima, mínima e média, precipitação pluviométrica e umidade relativa do ar foram coletadas entre 1991 e 2004. A incidência da doença foi avaliada mensalmente em 2004 em todas as fases de produção de mudas clonais de eucalipto. A presença do patógeno foi avaliada na água coletada do efluente de fertirrigação. A incidência do mofo-cinzento correlacionou-se melhor e negativamente com a temperatura máxima. Os resultados indicaram que a temperatura máxima é a variável a ser monitorada para fins de previsão da doença, a qual apresenta alto risco de incidência quando a temperatura máxima registrada for inferior a 27 ºC. Dentre os 14 clones propagados em 2004, o clone 957 (híbrido de Eucalyptus urophylla) apresentou menor incidência da doença, sob condições de infecção natural. Observou-se que o fungo encontra-se comumente associado a mudas de eucalipto e que o desenvolvimento da epidemia é regulado por temperaturas amenas, uma vez que, condições de alta umidade relativa e presença de água livre no hospedeiro ocorrem constantemente, em virtude das freqüentes irrigações requeridas para produção de mudas. Constatou-se que a água reutilizada, coletada no efluente de irrigação, contém inóculo do patógeno.
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    Effectiveness of systemic fungicides in the control of Quambalaria eucalypti and their effects on production of eucalypt mini-cuttings for rooting
    (Crop Protection, 2008-02) Ferreira, E. M.; Alfenas, A. C.; Maffia, L. A.; Mafia, R. G.; Mounteer, A. H.
    Eucalypt leaf spot caused by Quambalaria eucalypti is currently one of the main diseases occurring in mini-clonal hedges cultivated in sand beds with drip fert-irrigation or hydroponic tanks. Given the lack of studies on the effectiveness of fungicides for control of this pathogen, the protective, curative and antisporulating effects of systemic fungicides and their influence on production and rooting of eucalypt mini-cuttings were evaluated. Among the nine active ingredients tested, azoxystrobin, epoxiconazole, epoxiconazole+pyraclostrobin, pyraclostrobin and tebuconazole were the most effective against Q. eucalypti. Epoxiconazole, epoxiconazole+pyraclostrobin and tebuconazole presented protective, curative and antisporulating effects against the pathogen. In mini-clonal hedges, only epoxiconazole+pyraclostrobin negatively affected the production of mini-cuttings.
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    Root colonization and interaction among growth promoting rhizobacteria isolates and eucalypts species
    (Revista Árvore, 2009-01-26) Alfenas, Acelino Couto; Mafia, Reginaldo Gonçalves; Ferreira, Eraclides Maria; Binoti, Daniel Henrique Breda; Mafia, Gizella Machado Ventura; Mounteer, Ann Honor
    This work aimed to evaluate root colonization and interaction among isolates of rhizobacteria and eucalypt species. The method used to evaluate "in vitro" root colonization was able to indicate if the effect was benefic or deleterious allowing to pre-select isolates as potential growth promoter. There was interaction among isolates of rhizobacteria and Eucalyptus species for seed germinating and seedling growth. MF2 (Pseudomonas sp.) was the best rhizobacteria isolate for growth promotion of E. cloeziana e E. grandis. S1 (Bacillus subtilis) was the most effective for E. globulus, and Ca (Pseudomonas fulva), MF2 (Pseudomonas sp.), CIIb (Stenotrophomonas maltophilia) and S2 (B. subtilis) were the most promising isolates for the E. urophylla.
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    Efeito de rizobabactérias sobre o enraizamento e crescimento de clones de eucalipto em diferentes condições de propagação clonal
    (Revista Árvore, 2007-03-29) Mafia, Reginaldo Gonçalves; Alfenas, Acelino Couto; Maffia, Luiz Antônio; Ferreira, Eraclides Maria; Siqueira
    Objetivou-se, com este trabalho, avaliar a eficiência de isolados de rizobactérias sobre o enraizamento e crescimento de clones de eucalipto sob diferentes condições de propagação clonal. Os resultados evidenciaram que os incrementos em enraizamento e em biomassa radicular variaram de acordo com o isolado de rizobactéria e o clone de eucalipto, não sendo observado efeito deletério em nenhuma das combinações testadas. O ganho médio no enraizamento e biomassa radicular foi de 20,4 e 73,0%, respectivamente. Para enraizamento, o isolado mais promissor foi o S2 e na biomassa radicular, os isolados S1 e S2.