Mulching films affecting soil bacterial and fungal communities in a drip-irrigated potato soil

Abstract

Film mulching is an effective water-saving and yield-increasing measure for potato production in Northwest China. However, the response mechanism of microbial communities to mulching films in the soil is still unclear. In this study, polyethylene film mulching (PM), biodegradable film mulching (BM), liquid film mulching (LM), and non-mulching (NM) were applied on the drip-irrigated soil to investigate the effects of mulching films on soil bacterial and fungal communities through DNA sequencing, Pearson correlation analysis, and redundancy analysis. The results showed that LM treatment significantly increased the contents of soil mineral N (SMN), dissolved organic carbon (DOC), and dissolved organic nitrogen (DON) (p<0.05) in comparison with NM. The soil treated with LM presented high bacterial OTUs (operational taxonomic units), Chao1, ACE, and Shannon indices; however, the same indexes of fungi were low in LM and BM treatments. At the phylum level, Proteobacteria, Actinobacteria, and Chloroflexi were dominant bacterial communities. The LM treatment increased the OTUs of Proteobacteria; PM treatment increased the OTUs of Actinobacteria and Chloroflexi. Ascomycota was the dominant fungal community, which were decreased in soil under mulching films. In terms of soil properties, DON was closely correlated (p<0.05) with the microbial OTUs, Chao1, and ACE indices. The DOC and SWC (soil water content) contributed 51.2 % to the change of bacterial structure; however, the fungal structure was less sensitive to the variation of soil properties. Our results indicate that liquid film mulching favors increasing the diversity and abundance of dominant bacterial species, which were associated with the variation of soil properties.