Cloning and characterization of a gene encoding the endopolygalacturonase of Penicillium griseoroseum
| dc.contributor.author | Ribon, A. O. B. | |
| dc.contributor.author | Coelho, J. L. C. | |
| dc.contributor.author | Barros, E. G. de | |
| dc.contributor.author | Araújo, E. F. | |
| dc.date.accessioned | 2018-11-29T17:14:44Z | |
| dc.date.available | 2018-11-29T17:14:44Z | |
| dc.date.issued | 1999-05 | |
| dc.description.abstract | A conserved region of a polygalacturonase (PG) gene from Penicillium griseoroseum was PCR amplified and used to screen a genomic library from this fungus. The nucleotide sequence of the isolated clone (pggI) consisted of 1497 bp, including a coding region of 1251 bp. This region potentially encodes a protein of 376 amino acids, and is interrupted by two introns. Extensive homology was observed between this protein and several fungal endopolygalacturonases. DNA hybridization analyses revealed that there is a low copy number of pggI in the P. griseoroseum genome, probably one or two copies. | en |
| dc.format | pt-BR | |
| dc.identifier.issn | 15736776 | |
| dc.identifier.uri | http://dx.doi.org/10.1023/A:1005419225412 | |
| dc.identifier.uri | http://www.locus.ufv.br/handle/123456789/22655 | |
| dc.language.iso | eng | pt-BR |
| dc.publisher | Biotechnology Letters | pt-BR |
| dc.relation.ispartofseries | Volume 21, Issue 5, Pages 395– 399, May 1999 | pt-BR |
| dc.rights | 1999 Kluwer Academic Publishers. Printed in the Netherlands. | pt-BR |
| dc.subject | Filamentous fungi | pt-BR |
| dc.subject | Gene cloning | pt-BR |
| dc.subject | Pectinase | pt-BR |
| dc.subject | Sequence homology | pt-BR |
| dc.title | Cloning and characterization of a gene encoding the endopolygalacturonase of Penicillium griseoroseum | en |
| dc.type | Artigo | pt-BR |