Production and regeneration of protoplasts from the mycorrhizal fungus Suillus granulatus
Arquivos
Data
1996-04-11
Título da Revista
ISSN da Revista
Título de Volume
Editor
World Journal of Microbiology and Biotechnology
Resumo
Experiments were performed with the mycorrhizal fungus Suillus granulatus to define the parameters for production and regeneration of protoplasts. Protoplasts were released at frequencies between 1 and 3×107/ml from mycelium 3 to 7 days old. The best osmotic stabilizer for protoplast release was MgSO4 (0.7 m). To optimize protoplast release and regeneration an enzyme (Novozym 234) concentration 1.7 mg/ml was chosen, with a digestion time of 1 to 2 h. Regenerated colonies formed mycorrhizae within 60 days after inoculation in Pinus caribaea var. hondurensis seedlings.
Descrição
Palavras-chave
Mycorrhizae, Protoplasts, Suillus