Characterization of CRISPR-Cas systems in the Ralstonia solanacearum species complex

dc.contributor.authorXavier, André da Silva
dc.contributor.authorAlmeida, Juliana Cristina Fraleon de
dc.contributor.authorMelo, Alessandra Gonçalves de
dc.contributor.authorRousseau, Geneviève M.
dc.contributor.authorTremblay, Denise M.
dc.contributor.authorRezende, Rafael Reis de
dc.contributor.authorMoineau, Sylvain
dc.contributor.authorAlfenas‐Zerbini, Poliane
dc.date.accessioned2019-03-14T18:06:29Z
dc.date.available2019-03-14T18:06:29Z
dc.date.issued2019-02
dc.description.abstractClustered regularly interspaced short palindromic repeats (CRISPRs) are composed of an array of short DNA repeat sequences separated by unique spacer sequences that are flanked by associated (Cas) genes. CRISPR-Cas systems are found in the genomes of several microbes and can act as an adaptive immune mechanism against invading foreign nucleic acids, such as phage genomes. Here, we studied the CRISPRCas systems in plant-pathogenic bacteria of the Ralstonia sola- nacearum species complex (RSSC). A CRISPR-Cas system was found in 31% of RSSC genomes present in public databases. Specifically, CRISPR-Cas types I-E and II-C were found, with I-E being the most common. The presence of the same CRISPRCas types in distinct Ralstonia phylotypes and species suggests the acquisition of the system by a common ancestor before Ralstonia species segregation. In addition, a Cas1 phylogeny (I-E type) showed a perfect geographical segregation of phylotypes, supporting an ancient acquisition. Ralstonia solanacearum strains CFBP2957 and K60 T were challenged with a virulent phage, and the CRISPR arrays of bacteriophage insensitive mutants (BIMs) were analysed. No new spacer acquisition was detected in the analysed BIMs. The functionality of the CRISPR-Cas interference step was also tested in R. solanacearum CFBP2957 using a spacer-protospacer adjacent motif (PAM) delivery system, and no resistance was observed against phage phiAP1. Our results show that the CRISPR-Cas system in R. solanacearum CFBP2957 is not its primary antiviral strategy.en
dc.formatpdfpt-BR
dc.identifier.issn13643703
dc.identifier.urihttps://doi.org/10.1111/mpp.12750
dc.identifier.urihttp://www.locus.ufv.br/handle/123456789/23952
dc.language.isoengpt-BR
dc.publisherMolecular Plant Pathologypt-BR
dc.relation.ispartofseriesVolume 20, Issue 02, Pages 223-239, February 2019pt-BR
dc.rightsOpen Accesspt-BR
dc.subjectAdaptive immunitypt-BR
dc.subjectTranscriptional controlpt-BR
dc.subjectBacteria-virus interactionpt-BR
dc.titleCharacterization of CRISPR-Cas systems in the Ralstonia solanacearum species complexen
dc.typeArtigopt-BR

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