Taxa de prenhez e concentração sérica de progesterona em novilhas receptoras de embrião tratadas com somatotropina recombinante bovina (RBST)
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2007-01
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Revista Ceres
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O objetivo deste trabalho foi avaliar o efeito da aplicação de somatotropina recombinante bovina (rbST) sobre
a taxa de prenhez e a concentração sérica de progesterona (P 4 ) em novilhas receptoras de embrião. Foram selecionadas
52 novilhas como receptoras, distribuídas aleatoriamente em dois grupos (G1 e G2), sendo o G1 composto por 22
novilhas que não receberam rbST (controle), e o G2 por 30 novilhas que receberam 250 mg de rbST, por via
subcutânea, na fossa ísqueo-retal, durante o estro. As novilhas de ambos grupos foram inovuladas entre o sexto e
oitavo dia do ciclo estral com embriões recém-coletados. No momento da transferência dos embriões, colheu-se
sangue da veia ou artéria coccígea, para análise da concentração sérica de P 4 , pela técnica de radioimunoensaio. A
taxa de prenhez foi determinada por exame ultra-sonográfico realizado no 28o dia após a detecção do estro das
receptoras. Foram observadas 14 novilhas gestantes (46,6%) no G2 e 11 novilhas gestantes (50,0%) no G1. A rbST
não afetou a taxa de gestação das novilhas receptoras (P>0,05). A concentração média de P 4 no dia da inovulação
dos embriões foi de 1,71±0,74 ng/mL para os animais de G1 (n=22) e de 1,48±0,72 ng/mL para os de G2 (n=27), não
havendo diferença (P>0,05). Concluiu-se que a aplicação de 250 mg de rbST, durante o estro, em novilhas receptoras
de embrião não afetou a concentração sérica de P 4 entre o sexto e oitavo dia, nem a taxa de prenhez no 28o dia após
o estro.
The aim of this work was to evaluate the effects of recombinant bovine somatotropin (rbST) on pregnancy rate and progesterone (P4) serum concentration in embryo recipient heifers. Fifty two heifers were selected as recipients and randomly allocated into two groups, (G1 and G2), where the G1 was composed by 22 heifers which were not injected with rbST (control), and the G2 by 30 heifers, which were treated with a single subcutaneous injection of 250 mg of rbST, applied at detected estrus. Heifers from both treatments received fresh embryos, from day six to eight after the detected estrus. At the moment of embryo transfer, blood samples were collected from the coccygeal vein or artery for serum P4 analysis using the radioimmunoassay technique. The pregnancy rate was established by ultrasound exam performed twenty eight day after detected estrus of the receptors. No significant difference (P>0.05) was observed in the pregnancy rate between G1 (50%) and G2 (46.6%) treated heifers, or in the P4 concentration, with mean values of 1.71±0.74 ng/mL, and 1.48±0.72 ng/mL for G1 (n=22) and G2 (n=27) treated animals, respectively. In conclusion, the application of 250 mg rbST, at detected estrus in heifers, had no effect between days six to eight on P4 serum concentration or on pregnancy rate twenty-eight day after detected estrus.
The aim of this work was to evaluate the effects of recombinant bovine somatotropin (rbST) on pregnancy rate and progesterone (P4) serum concentration in embryo recipient heifers. Fifty two heifers were selected as recipients and randomly allocated into two groups, (G1 and G2), where the G1 was composed by 22 heifers which were not injected with rbST (control), and the G2 by 30 heifers, which were treated with a single subcutaneous injection of 250 mg of rbST, applied at detected estrus. Heifers from both treatments received fresh embryos, from day six to eight after the detected estrus. At the moment of embryo transfer, blood samples were collected from the coccygeal vein or artery for serum P4 analysis using the radioimmunoassay technique. The pregnancy rate was established by ultrasound exam performed twenty eight day after detected estrus of the receptors. No significant difference (P>0.05) was observed in the pregnancy rate between G1 (50%) and G2 (46.6%) treated heifers, or in the P4 concentration, with mean values of 1.71±0.74 ng/mL, and 1.48±0.72 ng/mL for G1 (n=22) and G2 (n=27) treated animals, respectively. In conclusion, the application of 250 mg rbST, at detected estrus in heifers, had no effect between days six to eight on P4 serum concentration or on pregnancy rate twenty-eight day after detected estrus.
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Palavras-chave
Fêmea, Hormônio, Reprodução