Fitopatologia - Artigos

URI permanente para esta coleçãohttps://locus.ufv.br/handle/123456789/11741

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Autor: search.filters.author.Zerbini, F. MuriloData final: 2019Tem arquivos: Sim

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Agora exibindo 1 - 10 de 27
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    Molecular characterization of two brazilian isolates of Lettuce mosaic virus with distinct biological properties
    (Fitopatologia Brasileira, 2001-06) Krause- Sakate, Renate; Mello, Raquel N.; Pavan, Marcelo A.; Zambolim, Eunize M.; Carvalho, Murilo G.; Gall, Olivier Le; Zerbini, F. Murilo; Krause-Sakate, Renate
    The coat protein genes of two field isolates of Lettuce mosaic virus (LMV) from São Paulo State, previously characterized based on their virulence on lettuce (Lactuca sativa) differential cultivars as belonging to pathotypes II (isolate AF198, unable to infect cultivars possessing the genes mo11 or mo12) and IV (isolate AF199, which breaks the resistance conferred by mo11 or mo12), were cloned and sequenced. Comparisons of the nucleotide sequences from European, Middle-Eastern, North American, and the two Brazilian isolates did not distinguish strains, because homologies were always greater than 95%. However, phylogenetic analysis indicated that the Brazilian isolate AF198 clusters with isolates LMV-R and LMV-0 (pathotype II, from the United States and France, respectively). Isolate AF199 clustered with two isolates (LMV-Aud and LMV-13) from France. These isolates are also closely related to isolates from Chile, although a common origin is not proposed. Independent mutation events may be occurring in different parts of the world, leading to the emergence of distinct LMV strains capable of overcoming the resistance genes mo11 or mo12.
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    Variabilidade genética de isolados de badnavírus infectando inhame (Dioscorea spp.) no Nordeste do Brasil
    (Tropical Plant Pathology, 2013-07) Lima, Joyce Silva; Lima, Alison T. M.; Castillo- Urquiza, Gloria P.; Silva, Sarah J. C.; Assunção, Iraildes P.; Michereff, Sami J.; Zerbini, F. Murilo; Lima, Gaus S. A.
    Viroses causadas por vírus do gênero Badnavirus são responsáveis por grandes prejuízos à cultura do inhame no Nordeste brasileiro. O conhecimento da variabilidade destes patógenos pode fornecer informações importantes sobre seu potencial evolutivo, permitindo a elaboração de melhores estratégias de manejo da doença. A análise de 425 amostras foliares de inhame coletadas em três estados do Nordeste brasileiro, em 2010, revelou uma alta incidência (93,3%) de badnaviroses. Para avaliar a variabilidade genética dos badnavírus infectando inhame, um fragmento de 579 nucleotídeos correspondente à região codificante da transcriptase reversa (RT)/RNaseH dos isolados amostrados foi amplificada por PCR e sequenciada. A análise filogenética das sequências de nucleotídeos revelou que os isolados dividem-se em dois grupos. Um é altamente relacionado com Dioscorea bacilliform AL virus (DBALV), enquanto o outro forma um clado altamente divergente dentro do gênero Badnavirus. Os isolados de DBALV apresentam 70-98% de identidade nucleotídica entre si e foram detectados em todas as áreas avaliadas e em D. alata e D. cayennensis, as duas espécies de inhame mais cultivadas no Nordeste. Os isolados do outro grupo compartilham 47-58% de identidade com isolados de DBALV e 78-95% entre si e foram encontrados apenas em D. alata na Paraíba.
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    Caracterização de um isolado do Bean rugose mosaic virus (BRMV) de Minas Gerais e estimativa de perdas em feijoeiro em infecção simples ou em conjunto com o BCMV
    (Fitopatologia Brasileira, 2006-09) Maia, Felipe G. M.; Carvalho, Murilo G.; Pinto, Cleide M. F.; Zerbini, F. Murilo; Castillo-Urquiza, Gloria P.
    Plantas de feijão-vagem do cultivar Novirex apresentando mosaico e enrolamento de vagens, sem deformação foliar evidente, foram coletadas em 2002 em Cordisburgo, MG. Estudos preliminares identificaram o vírus como um isolado do Bean rugose mosaic virus (BRMV). Este trabalho relata a caracterização do isolado, por meio de produção e avaliação de anti-soro, determinação da gama de hospedeiros, estudo da transmissão do vírus por besouros crisomelídeos e estimativa de perdas em feijoeiro como resultado de infecção isolada ou em conjunto com o Bean common mosaic virus (BCMV). O roteiro adotado para purificação possibilitou a obtenção de vírus purificado em rendimento satisfatório para a produção de anti-soro. A titulação dos anti-soros foi realizada por ELISA indireto, obtendo-se reações positivas com a diluição máxima testada (1:70.000). Das 22 espécies vegetais utilizadas na gama de hospedeiros, foram infectadas plantas de Chenopodium quinoa e alguns cultivares de feijão e soja, conforme esperado para o BRMV. O isolado de BRMV foi transmitido pelo besouro crisomelídeo Cerotoma arcuata a uma taxa de 33,3%. A infecção simples de feijão 'Ouro Negro' e de feijão-vagem 'Novirex' levou a uma redução do peso das vagens por planta de 3,4% e 84,9%, respectivamente. Infecção mista do BRMV com o BCMV levou a uma redução do peso de vagens por planta de até 70,1% para 'Novirex' e de até 90,8% para 'Ouro Negro'.
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    Efeitos na fotossíntese e área foliar de cultivares de alface inoculadas mecanicamente com patótipos do Lettuce mosaic virus e Lettuce mottle virus
    (Fitopatologia Brasileira, 2004-01) Jadão, Adriana S.; Pavan, Marcelo A.; Krause-Sakate, Renate; Zerbini, F. Murilo
    Levantamentos realizados no estado de São Paulo indicaram a ocorrência isolada e em infecções mistas do Lettuce mosaic virus (LMV) e do Lettuce mottle virus (LeMoV) em plantas de alface (Lactuca sativa). O presente trabalho teve como objetivo estudar os efeitos da infecção isolada e mista entre o LMV (patótipos II e IV) e o LeMoV, em cultivares de alface suscetível (White Boston) e tolerante (Elisa - gene mol1) ao LMV patótipo II. As plantas foram inoculadas via extrato vegetal tamponado com isolados de LMV-II, LMV-IV e LeMoV separadamente e em diferentes combinações, com intervalo de 24 h ou simultaneamente com os dois vírus. As plantas infetadas foram analisadas utilizando-se hospedeiras diferenciais para o LMV e o LeMoV, e no caso do LMV pelo teste sorológico de PTA-ELISA. Nas avaliações de peso fresco e seco, área foliar e teor de clorofila, observou-se que a cultivar White Boston foi a mais afetada por ambos os vírus. As infecções mistas e isoladas na cultivar Elisa causaram efeitos semelhantes, provavelmente devido a presença do gene mo11 de tolerância ao LMV-II. O isolado LMV-IV foi considerado o mais agressivo nestas cultivares quando comparado ao LMV-II e o LeMoV.
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    Genetic diversity of begomoviruses infecting soybean, bean and associated weeds in Northwestern Argentina
    (Fitopatologia Brasileira, 2006-07) Rodríguez-Pardina, Patricia E.; Zerbini, F. Murilo; Ducasse, Daniel A.
    The subtropical Northwestern region of Argentina (provinces of Tucumán, Salta, Jujuy, Santiago del Estero and Catamarca) suffers from a high incidence of the whitefly Bemisia tabaci, and the detection of begomoviruses is also common. The Northwest is the main bean-growing region of the country, and approximately 10% of Argentina's soybean crop is grown in this area. We have used a PCR-based assay to establish the identity and genetic diversity of begomoviruses associated with bean and soybean crops in Northwestern Argentina. Universal begomovirus primers were used to direct the amplification of a fragment encompassing the 5' portion of the capsid protein gene. Amplified fragments were cloned, sequenced and subjected to phylogenetic analysis to determine the sequence identity to known begomoviruses. The data indicated the presence of four distinct begomoviruses, all related to other New World begomoviruses. The prevalent virus, which was present in 94% of bean and soybean samples and also in two weed species, is closely related to Sida mottle virus (SiMoV). A virus with high sequence identity with Bean golden mosaic virus (BGMV) was found in beans. The two remaining viruses displayed less than 89% identity with other known begomoviruses, indicating that they may constitute novel species. One of these putative novel viruses was detected in bean, soybean and tomato samples.
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    World management of geminiviruses
    (Annual Review of Phytopathology, 2018) Rojas, Maria R.; Macedo, Monica A.; Maliano, Minor R.; Soto-Aguilar, Maria; Souza, Juliana O.; Briddon, Rob W.; Kenyon, Lawrence; Bustamante, Rafael F. Rivera; Zerbini, F. Murilo; Adkins, Scott; Legg, James P.; Kvarnheden, Anders; Wintermantel, William M.; Sudarshana, Mysore R.; Peterschmitt, Michel; Lapidot, Moshe; Martin, Darren P.; et al.
    Management of geminiviruses is a worldwide challenge because of the widespread distribution of economically important diseases caused by these viruses. Regardless of the type of agriculture, management is most effective with an integrated pest management (IPM) approach that involves measures before, during, and after the growing season. This includes starting with resistant cultivars and virus- and vector-free transplants and ropagative plants. For high value vegetables, protected culture (e.g., greenhouses and screenhouses) allows for effective management but is limited owing to high cost. Protection of young plants in open fields is provided by row covers, but other measures are typically required. Measures that are used for crops in open fields include roguing infected plants and insect vector management. Application of insecticide to manage vectors (whiteflies and leafhoppers) is the most widely used measure but can cause undesirable environmental and human health issues. For annual crops, these measures can be more effective when combined with host-free periods of two to three months. Finally, given the great diversity of the viruses, their insect vectors, and the crops affected, IPM approaches need to be based on the biology and ecology of the virus and vector and the crop production system. Here, we present the general measures that can be used in an IPM program for geminivirus diseases, specific case studies, and future challenges.
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    Taxonomy of the order Mononegavirales: second update 2018
    (Archives of Virology, 2019-04) Zerbini, F. Murilo; Maes, Piet; Amarasinghe, Gaya K.; Ayllón, María A.; Basler, Christopher F.; Bavari, Sina; Blasdell, Kim R.; Briese, Thomas; Brown, Paul A.; Bukreyev, Alexander; Balkema-Buschmann, Anne; Buchholz, Ursula J.; Chandran, Kartik; Crozier, Ian; Swart, Rik L. de; Dietzgen, Ralf G.; Dolnik, Olga; Domier, Leslie L.
    In October 2018, the order Mononegavirales was amended by the establishment of three new families and three new genera, abolishment of two genera, and creation of 28 novel species. This article presents the updated taxonomy of the order Mononegavirales as now accepted by the International Committee on Taxonomy of Viruses (ICTV).
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    Taxonomy of the order Bunyavirales: second update 2018
    (Archives of Virology, 2019-03) Zerbini, F. Murilo; Maes, Piet; Adkins, Scott; Alkhovsky, Sergey V.; Avšič-Županc, Tatjana; Ballinger, Matthew J.; Bente, Dennis A.; Beer, Martin; Bergeron, Éric; Blair, Carol D.; Briese, Thomas; Buchmeier, Michael J.; Burt, Felicity J.; Calisher, Charles H.; Charrel, Rémi N.; Choi, Il Ryong; Clegg, J. Christopher S.; Torre, Juan Carlos de la
    In October 2018, the order Bunyavirales was amended by inclusion of the family renaviridae, abolishment of three families, creation of three new families, 19 new genera, and 14 new species, and renaming of three genera and 22 species. This article presents the updated taxonomy of the order Bunyavirales as now accepted by the International Committee on Taxonomy of Viruses (ICTV).
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    Revisiting the classification of curtoviruses based on genome-wide pairwise identity
    (Archives of Virology, 2014-01-25) Zerbini, F. Murilo; Varsani, Arvind; Martin, Darren P.; Navas-Castillo, Jesús; Moriones, Enrique; Hernández-Zepeda, Cecilia; Idris, Ali; Brown, Judith K.
    Members of the genus Curtovirus (family Geminiviridae) are important pathogens of many wild and cultivated plant species. Until recently, relatively few full curtovirus genomes have been characterised. However, with the 19 full genome sequences now available in public databases, we revisit the proposed curtovirus species and strain classification criteria. Using pairwise identities coupled with phylogenetic evidence, revised species and strain demarcation guidelines have been instituted. Specifically, we have established 77 % genome-wide pairwise identity as a species demarcation threshold and 94 % genome-wide pairwise identity as a strain demarcation threshold. Hence, whereas curtovirus sequences with >77 % genome-wide pairwise identity would be classified as belonging to the same species, those sharing >94 % identity would be classified as belonging to the same strain. We provide step-by-step guidelines to facilitate the classification of newly discovered curtovirus full genome sequences and a set of defined criteria for naming new species and strains. The revision yields three curtovirus species: Beet curly top virus (BCTV), Spinach severe surly top virus (SpSCTV) and Horseradish curly top virus (HrCTV).
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    Molecular mapping of the viral determinants of systemic wilting induced by a Lettuce mosaic virus (LMV) isolate in some lettuce cultivars
    (Virus Research, 2005-05) Zerbini, F. Murilo; Krause-Sakate, Renate; Redondo, Elise; Richard-Forget, Florence; Jadão, Adriana Salomão; German-Retana, Sylvie; Pavan, Marcelo Agenor; Candresse, Thierry; Gall, Olivier Le; Houvenaghel, Marie Christine
    The isolate AF199 of Lettuce mosaic virus (LMV, genus Potyvirus) causes local lesions followed by systemic wilting and plant death in the lettuce cultivars Ithaca and Vanguard 75. Analysis of the phenotype of virus chimeras revealed that a region within the P1 protein coding region (nucleotides 112–386 in the viral genome) and/or another one within the CI protein coding region (nucleotides 5496–5855) are sufficient together to cause the lethal wilting in Ithaca, but not in Vanguard 75. This indicates that the determinants of this particular symptom are different in these two lettuce cultivars. The wilting phenotype was not directly correlated with differences in the deduced amino acid sequence of these two regions. Furthermore, transient expression of the LMV-AF199 proteins, separately or in combination, did not induce local necrosis or any other visible reaction in the plants. Together, these results suggest that the systemic wilting reaction might be due to RNA rather than protein sequences.