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URI permanente para esta coleçãohttps://locus.ufv.br/handle/123456789/11852

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Comparação entre duas concentrações de glicerol para a criopreservação de sêmen de suçuarana (Puma concolor)
(Pesquisa Veterinária Brasileira, 2013-04) Deco-Souza, Thyara de; Paula, Tarcizio A. R. de; Costa, Deiler Sampaio; Costa, Eduardo Paulino da; Barros, João Bosco Gonçalves de; Araujo, Gediendson Ribeiro de; Carreta-Jr, Moacir
O desenvolvimento de biotécnicas de reprodução é uma importante ferramenta para a conservação de animais silvestres ameaçados de extinção. Procedimentos de reprodução assistida em suçuarana, no entanto, são escassos na literatura, em especial aqueles relacionados à criopreservação de sêmen. Neste sentido, o presente trabalho objetivou avaliar a congelabilidade do sêmen de suçuaranas adultas mantidas em cativeiro, por meio da comparação entre duas concentrações de glicerol no meio de congelamento. Foram usados cinco machos adultos de suçuarana, mantidos no Centro de Reabilitação de Animais Silvestres do Mato Grosso do Sul (CRAS/MS). As amostras foram coletadas por eletroejaculação e avaliadas quanto ao seu aspecto físico, volume, vigor, motilidade, concentração e índice espermático. De cada ejaculado duas alíquotas foram diluídas em meio Tris-citrato-gema de ovo, em concentrações finais de 5 e 7,5% de glicerol, resfriadas a uma taxa de -0,55ºC/min e congeladas a uma taxa de -5,8ºC/min. Depois de descongeladas, as amostras foram reavaliadas e submetidas aos testes de termorresistência e hiposmótico. O protocolo de criopreservação e descongelamento de sêmen proposto se mostrou eficiente em ambas as concentrações de glicerol testadas, não havendo diferença (p>0,05) entre estas.
Effect of somatotropin and thyroxine on the in vitro development of bovine preantral follicles
(Ciência Animal Brasileira, 2018) Silva, Talita Fernandes da; Costa, Sanely Lourenço da; Costa, Eduardo Paulino da; Guimarães, José Domingos
The aim of the study was to evaluate the effect of recombinant bovine somatotropin (rbST) and thyroxine (T4) on survival and growth of bovine preantral ovarian follicles (PAOF) cultured in vitro. Ovarian fragments were collected in local abattoirs and immediately fixed for classical histology and transmission electron microscopy (non-cultured control). The other fragments were then cultured in situ for seven days in minimum essential medium alone (MEM+ - cultured control) or in the presence of 1,000 ng/mL rbST and 20 ng/mL T4, isolated or associated. After seven days, there was a reduction (P<0.05) in the percentage of normal follicles in MEM+ alone or with T4. In oocyte diameter, there was a reduction in MEM+ alone. There was no influence (P>0.01) of the medium used on the follicular diameter of the PAOF cultured for seven days. Ultrastructural analysis showed cell damage. In conclusion, the presence of rbST maintains the rate of morphologically normal follicles during the culture for seven days (observed by optical microscopy), but it does not exert beneficial effects on its ultrastructural integrity and oocyte and follicular growth.
Natural infection in ovarian structures by bovine herpesvirus 1: molecular and serological detection
(Semina: Ciências Agrárias, 2015-03) Pereira, Emílio César Martins; Costa, Eduardo Paulino da; Silva Júnior, Abelardo; Mendes, Vívian Rachel de Araújo; Santos, Giancarlo Magalhães dos; Costa, Sanely Lourenço da; Santos, Marcus Rebouças
In this study, the polymerase chain reaction (PCR) was used to evaluate the presence of viral DNA in ovarian tissue, in the cumulus-oocyte complex (COC), follicular liquid, and blood of animals naturally infected with bovine herpesvirus-1 (BoHV-1). The serum profile of the sampled animals was also evaluated. Samples of serum, blood, ovarian tissue, follicular liquid, and COC were collected from 147 slaughterhouse animals that were not vaccinated against BoHV-1. Contaminated or insufficient samples were disregarded. Serological tests allowed the identification of serum-positive animals with neutralizing antibodies against BoHV-1. Analysis of samples by PCR revealed the presence of viral DNA in 0.9% (1/115) of the COC samples, in 4.3% (5/117) of the ovarian tissue samples, and in 2.8% (3/108) of the blood samples. Viral DNA was not detected in any of the follicular liquid samples. In serological samples, a positivity of 83.6% (117/140) was observed for BoHV-1. All PCR-positive animals, regardless of the samples analyzed, showed positivity in the serum neutralization test for the detection of BoHV-1-specific antibodies. According to these results, a high prevalence of antibodies against BoHV-1 was detected in naturally infected animals from different herds, and the molecular tests revealed the presence of viral DNA in bovine ovarian tissue, providing evidence that this might be a site of BoHV-1 infection in naturally infected animals.
Relationship of testicular biometry with semen variables in breeding soundness evaluation of Nellore bulls
(Animal Reproduction Science, 2018-09) Penitente-Filho, Jurandy Mauro; Silva, Fabyano Fonseca e; Guimarães, Simone Facioni; Waddington, Bruna; Costa, Eduardo Paulino da; León, Victor Gomez; Siqueira, Jeanne Broch; Okano, Denise Silva; Maitan, Paula Piccolo; Guimarães, José Domingos
This study aimed to evaluate the correlation between testicular biometry and semen variables, as well as, to relate testicular variables to the probability of selecting Nellore bulls with desirable sperm morphology when conducting breeding soundness evaluations (BSE). A total of 2055 BSEs from 506 bulls comprised the dataset. Biometric variables evaluated were: scrotal circumference, testicular volume, width, length, ratio and eccentricity; and semen variables were sperm motility, major sperm defects, minor sperm defects and normal sperm. Data of testicular biometry were correlated with data for semen variables using the Pearson’s correlation assessment. Effects of testicular variables in selecting for sperm morphology of bulls in the BSE were evaluated by logistic regression. Scrotal circumference, testicular volume, length and width were positively correlated to sperm motility (0.18 to 0.19) and normal sperm (0.24 to 0.27) and negatively correlated with values for major defects (−0.24 to −0.27), but for testicular ratio and eccentricity there were coefficients near zero for all semen traits. Testicular ratio and eccentricity were not suitable for predicting the probability of selecting a bull based on semen variables using the BSE, but scrotal circumference, testicular volume, length and width were highly significant (P < 0.0001) with moderate values of area under ROC (Receiver Operating Characteristics) curve (0.608 to 0.620).
Taxa de prenhez e concentração sérica de progesterona em novilhas receptoras de embrião tratadas com somatotropina recombinante bovina (RBST)
(Revista Ceres, 2007-01) Haas, Giorgia Thaís da Silva; Fernandes, Carlos Antônio de Carvalho; Costa, Eduardo Paulino da; Torres, Ciro Alexandre Alves; Marques, Paulo Alexandre Fernandes; Lopes, Flávio Guiselli; Paula, Tarcízio Antônio Rêgo de
O objetivo deste trabalho foi avaliar o efeito da aplicação de somatotropina recombinante bovina (rbST) sobre a taxa de prenhez e a concentração sérica de progesterona (P 4 ) em novilhas receptoras de embrião. Foram selecionadas 52 novilhas como receptoras, distribuídas aleatoriamente em dois grupos (G1 e G2), sendo o G1 composto por 22 novilhas que não receberam rbST (controle), e o G2 por 30 novilhas que receberam 250 mg de rbST, por via subcutânea, na fossa ísqueo-retal, durante o estro. As novilhas de ambos grupos foram inovuladas entre o sexto e oitavo dia do ciclo estral com embriões recém-coletados. No momento da transferência dos embriões, colheu-se sangue da veia ou artéria coccígea, para análise da concentração sérica de P 4 , pela técnica de radioimunoensaio. A taxa de prenhez foi determinada por exame ultra-sonográfico realizado no 28o dia após a detecção do estro das receptoras. Foram observadas 14 novilhas gestantes (46,6%) no G2 e 11 novilhas gestantes (50,0%) no G1. A rbST não afetou a taxa de gestação das novilhas receptoras (P>0,05). A concentração média de P 4 no dia da inovulação dos embriões foi de 1,71±0,74 ng/mL para os animais de G1 (n=22) e de 1,48±0,72 ng/mL para os de G2 (n=27), não havendo diferença (P>0,05). Concluiu-se que a aplicação de 250 mg de rbST, durante o estro, em novilhas receptoras de embrião não afetou a concentração sérica de P 4 entre o sexto e oitavo dia, nem a taxa de prenhez no 28o dia após o estro.
Biometria macro e microscópica dos componentes testiculares em Lobo guará (Chrysocyon brachyurus, Illiger, 1811) adulto
(Revista Ceres, 2007-05) Bittencourt, Viviane Lewicki; Paula, Tarcízio Antônio Rego de; Matta, Sergio Luiz Pinto da; Fonseca, Cláudio César; Costa, Deiler Sampaio; Costa, Eduardo Paulino da; Benjamin, Laércio dos Anjos
Os parâmetros de biometria macro e microscópicas testicular auxiliam no desenvolvimento de protocolos para reprodução assistida nas diferentes espécies. Foram estudados no presente experimento quatro lobos guarás adultos provenientes de cativeiro, estes animais pesaram em média 31,5 kg com massa testicular média de 6,3 g tendo apresentado índice gonadossomático de 0,04%. O volume médio de túbulos seminíferos por testículo foi de 4,81 ml, o que representou cerca de 79,3% do parênquima testicular. Em relação à massa corporal, cerca de 0,03% desta é alocada em túbulos seminíferos, ou seja, o índice tubulossomático. O lobo guará apresentou cerca de 18 metros de túbulo seminífero por grama de testículo. O diâmetro do epitélio seminífero foi em média 227,3 mm. Os valores registrados neste estudo encontram-se dentro da amplitude observada para as demais espécies carnívoras já estudadas.
Exogenous induction of ovarian activity and ovulation and transfer of fresh embryos of domestic cat (Felis catus)
(Revista Ceres, 2012-07-06) Santana, Marcelo Lopes; Paula, Tarcízio Antônio Rego de; Costa, Eduardo Paulino da; Costa, Deiler Sampaio
The objective of the present study was the exogenous stimulation of ovarian activity and definition of embryo collection, and transfer protocols, in the domestic cat for potential application in non-domestic endangered species. Sixteen adult queens and two adult male reproducers kept in the experimental cat house at the Morphology sector at the Veterinary Department (DVT), UFV, were used in this study. All the queens received a single application of 150 IU Equine Chorionic Gonadotropin (eCG) in the post estrus to induce ovarian activity and 80 to 84 hours later, received a single application of 100 UI Human Chorionic Gonadotropin (hCG) to induce ovulation. After hCG application, only the donor queens were naturally mated. The receptor queens received extra stimulus for induction of ovulation through manipulation of an intravaginal swab. Five to six days after hCG application, the donor queens were subjected to a laparotomy for embryo collection that was performed by trans-horn uterine washing. On average, six embryos were surgically inovulated. They were classified as type I and III compact morula and blastocysts in four receptor queens. Three animals presented pregnancy confirmed by ultrasound at day 36 and two of these animals gave birth to litters of two and four offsprings, respectively, at 66 and 63 days after induction of ovulation. Except for one still birth, all the offspring developed normally.
Human follicle stimulating hormone (hFSH) and thyroxine (T4) in survival maintenance and in vitro growth promotion of caprine preantral follicles
(Ciência Animal Brasileira, 2015-02-23) Costa, Sanely Lourenço da; Costa, Eduardo Paulino da; Pereira, Emílio César Martins; Gonçalves, Wagner Gonzaga; Silva, Talita Fernandes da; Queiroz, Vanessa Lopes Dias
The aim of this study was to investigate the interaction of human FSH (10ng/ml) with T4 (20ng/mL) on survival, activation and growth of preantral follicles cultured in vitro for 28 days. Fragments of non-cultured and cultured ovarian tissue were processed for classic histology and transmission electron microscopy. The results showed a reduction in the survival rate in all the media tested (one to 28 days) when compared to the fresh control. However the treatment with T4/hFSH for seven days of culture maintained the rate similar to the control. The media tested by one and 28 days reduced the percentage of primordial follicles in all periods of culture. However, T4/hFSH on day one of culture remained similar to the fresh control. None of the media were able to keep the percentage of the developing follicles. It was observed that the follicular diameter in the medium with T4/hFSH remained similar to the fresh control. The ultrastructural analysis confirmed the integrity of follicles cultured for seven days in a medium supplemented with T4/hFSH. In conclusion, the medium with T4/hFSH is able to maintain the survival, promote the activation, and the ultrastructural integrity of caprine preantral follicles for until seven days.
The human follicle stimulating hormone (hFSH) keeps the normal ultrastructure of caprine preantral follicles cultured in vitro
(Semina: Ciências Agrárias, 2015-01-25) Costa, Sanely Lourenço da; Costa, Eduardo Paulino da; Pereira, Emílio César Martins; Benjamin, Laércio dos Anjos; Verde, Isabel Bezerra Lima; Celestino, Juliana Jales de Hollanda; Figueiredo, José Ricardo de
The aim of this study was to investigate the effects of human follicle stimulating hormone (hFSH) on the in vitro culture of caprine preantral follicles. Fragments of goat ovarian cortex were cultured in α-MEM + supplemented with 0, 10, or 50 ng/mL hFSH for one or seven days. Small fragments of non-cultured and cultured ovarian tissue were processed for classic histology and transmission electron microscopy. The statistical tests used in this study were Lilliefors, Cochran, and Tukey’s (when significance was detected, PROC ANOVA, SAS was used). The results revealed a reduction in the percentage of normal follicles after one or seven days of culture under all treatment conditions. The rates of follicular survival were similar to each other, within each day of culture. The medium containing 10 ng/mL hFSH reduced the percentage of primordial follicles following culture for one and seven days and did not increase the percentage of developing follicles. The follicular diameter of ovarian tissue cultured in α-MEM + medium and medium supplemented with 10 ng/mL of hFSH did not change when compared with the control (non-cultured). The ultrastructural analysis confirmed the integrity of follicles cultured for seven days in medium containing 10 ng/mL hFSH. In conclusion, hFSH (10 ng/mL) is capable of promoting the activation of primordial follicles and maintaining the ultrastructural integrity of caprine preantral follicles cultured in vitro for seven days.
Comparação entre duas concentrações de glicerol para a criopreservação de sêmen de suçuarana (Puma concolor)
(Pesquisa Veterinária Brasileira, 2012-09-18) Deco-Souza, Thyara de; Paula, Tarcizio A.R. de; Costa, Deiler Sampaio; Costa, Eduardo Paulino da; Barros, João Bosco Gonçalves de; Araujo, Gediendson Ribeiro de; Carreta-Jr, Moacir
O desenvolvimento de biotécnicas de reprodução é uma importante ferramenta para a conservação de animais silvestres ameaçados de extinção. Procedimentos de reprodução assistida em suçuarana, no entanto, são escassos na literatura, em especial aqueles relacionados à criopreservação de sêmen. Neste sentido, o presente trabalho objetivou avaliar a congelabilidade do sêmen de suçuaranas adultas mantidas em cativeiro, por meio da comparação entre duas concentrações de glicerol no meio de congelamento. Foram usados cinco machos adultos de suçuarana, mantidos no Centro de Reabilitação de Animais Silvestres do Mato Grosso do Sul (CRAS/MS). As amostras foram coletadas por eletroejaculação e avaliadas quanto ao seu aspecto físico, volume, vigor, motilidade, concentração e índice espermático. De cada ejaculado duas alíquotas foram diluídas em meio Tris-citrato-gema de ovo, em concentrações finais de 5 e 7,5% de glicerol, resfriadas a uma taxa de -0,55ºC/min e congeladas a uma taxa de -5,8ºC/min. Depois de descongeladas, as amostras foram reavaliadas e submetidas aos testes de termorresistência e hiposmótico. O protocolo de criopreservação e descongelamento de sêmen proposto se mostrou eficiente em ambas as concentrações de glicerol testadas, não havendo diferença (p>0,05) entre estas.

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