Downregulation of the δ-subunit reduces mitochondrial ATP synthase levels, alters respiration, and restricts growth and gametophyte development in Arabidopsis

dc.contributor.authorNunes-Nesi, Adriano
dc.contributor.authorGeisler, Daniela A.
dc.contributor.authorPäpke, Carola
dc.contributor.authorObata, Toshihiro
dc.contributor.authorMatthes, Annemarie
dc.contributor.authorSchneitz, Kay
dc.contributor.authorMaximova, Eugenia
dc.contributor.authorAraújo, Wagner L.
dc.contributor.authorFernie, Alisdair R.
dc.contributor.authorPersson, Staffan
dc.date.accessioned2019-03-25T12:24:24Z
dc.date.available2019-03-25T12:24:24Z
dc.date.issued2012-07
dc.description.abstractThe mitochondrial ATP synthase (F1Fo complex) is an evolutionary conserved multimeric protein complex that synthesizes the main bulk of cytosolic ATP, but the regulatory mechanisms of the subunits are only poorly understood in plants. In yeast, the δ-subunit links the membrane-embedded Fo part to the matrix-facing central stalk of F1. We used genetic interference and an inhibitor to investigate the molecular function and physiological impact of the δ-subunit in Arabidopsis thaliana. Delta mutants displayed both male and female gametophyte defects. RNA interference of delta resulted in growth retardation, reduced ATP synthase amounts, and increased alternative oxidase capacity and led to specific long-term increases in Ala and Gly levels. By contrast, inhibition of the complex using oligomycin triggered broad metabolic changes, affecting glycolysis and the tricarboxylic acid cycle, and led to a successive induction of transcripts for alternative respiratory pathways and for redox and biotic stress-related transcription factors. We conclude that (1) the δ-subunit is essential for male gametophyte development in Arabidopsis, (2) a disturbance of the ATP synthase appears to lead to an early transition phase and a long-term metabolic steady state, and (3) the observed long-term adjustments in mitochondrial metabolism are linked to reduced growth and deficiencies in gametophyte development.en
dc.formatpdfpt-BR
dc.identifier.issn1532298X
dc.identifier.urihttps://doi-org.ez35.periodicos.capes.gov.br/10.1105/tpc.112.099424
dc.identifier.urihttp://www.locus.ufv.br/handle/123456789/24081
dc.language.isoengpt-BR
dc.publisherThe Plant Cellpt-BR
dc.relation.ispartofseriesVolume 24, Issue 07, Pages 2792– 2811, July 2012pt-BR
dc.rightsOpen Accesspt-BR
dc.subjectATPpt-BR
dc.subjectGametophytept-BR
dc.subjectArabidopsispt-BR
dc.titleDownregulation of the δ-subunit reduces mitochondrial ATP synthase levels, alters respiration, and restricts growth and gametophyte development in Arabidopsisen
dc.typeArtigopt-BR

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