Caracterização bioquímica e histológica do fígado, rim e pulmão de Phrynops geoffroanus (Schweigger, 1812) capturados na Bacia do Paraopeba (Minas Gerais)
Loading...
Date
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Universidade Federal de Viçosa
Abstract
Phrynops geoffroanus é um quelônio semiaquático amplamente utilizado como bioindicador devido à sua sensibilidade a alterações ambientais e à capacidade de bioacumular contaminantes. Este estudo caracterizou os efeitos da exposição ambiental a metais pesados em indivíduos capturados na Bacia do Rio Paraopeba, em Brumadinho, Minas Gerais, por meio de análises de bioacumulação, estresse oxidativo e histologia de fígado, rim e pulmão. Foram avaliados dezesseis animais adultos, dos quais foram obtidos fragmentos dos três órgãos para determinação de metais por espectrometria de emissão óptica com plasma indutivamente acoplado (ICP-OES), análise da atividade das enzimas superóxido dismutase, catalase e glutationa S-transferase, quantificação de marcadores de dano oxidativo (malondialdeído e proteínas carboniladas) e avaliação histopatológica em lâminas coradas com hematoxilina e eosina. Detectou-se acúmulo expressivo de alumínio, ferro, cobre, chumbo e manganês nos tecidos, com destaque para as altas concentrações de alumínio e ferro. Os achados bioquímicos demonstraram desequilíbrio redox e aumento de biomoléculas oxidadas, enquanto as análises histológicas evidenciaram infiltrado inflamatório, degeneração celular e outras alterações morfofuncionais compatíveis com exposição crônica a contaminantes. Em conjunto, os resultados indicam que P. geoffroanus apresenta respostas fisiológicas e teciduais sensíveis à contaminação ambiental, reforçando seu potencial como bioindicador e destacando a importância do monitoramento ecotoxicológico na Bacia do Rio Paraopeba. Palavras-chave: bioacumulação; estresse oxidativo; bioindicadores ambientais; histopatologia.
Phrynops geoffroanus is a semiaquatic turtle widely used as a bioindicator due to its sensitivity to environmental disturbances and its capacity to bioaccumulate contaminants. This study characterized the effects of environmental exposure to heavy metals in individuals captured in the Paraopeba River Basin, in Brumadinho, Minas Gerais, through analyses of metal bioaccumulation, oxidative stress, and histology of the liver, kidney, and lung. Sixteen adult animals were evaluated, from which tissue fragments of the three organs were collected for metal quantification using inductively coupled plasma optical emission spectrometry, analysis of the activities of the enzymes superoxide dismutase, catalase, and glutathione S-transferase, quantification of oxidative damage markers (malondialdehyde and carbonylated proteins), and histopathological assessment of sections stained with hematoxylin and eosin. The results demonstrated substantial accumulation of aluminum, iron, copper, lead, and manganese in the tissues, with particularly high concentrations of aluminum and iron. The biochemical findings revealed redox imbalance and increased levels of oxidized biomolecules, whereas the histological analyses showed inflammatory infiltrates, cellular degeneration, and other morphofunctional alterations consistent with chronic exposure to environmental contaminants. Together, these results indicate that P. geoffroanus exhibits sensitive physiological and tissue- level responses to metal contamination, reinforcing its potential as an environmental bioindicator and highlighting the importance of continued ecotoxicological monitoring in the Paraopeba River. Keywords: bioaccumulation; oxidative stress; environmental bioindicators; histopathology.
Phrynops geoffroanus is a semiaquatic turtle widely used as a bioindicator due to its sensitivity to environmental disturbances and its capacity to bioaccumulate contaminants. This study characterized the effects of environmental exposure to heavy metals in individuals captured in the Paraopeba River Basin, in Brumadinho, Minas Gerais, through analyses of metal bioaccumulation, oxidative stress, and histology of the liver, kidney, and lung. Sixteen adult animals were evaluated, from which tissue fragments of the three organs were collected for metal quantification using inductively coupled plasma optical emission spectrometry, analysis of the activities of the enzymes superoxide dismutase, catalase, and glutathione S-transferase, quantification of oxidative damage markers (malondialdehyde and carbonylated proteins), and histopathological assessment of sections stained with hematoxylin and eosin. The results demonstrated substantial accumulation of aluminum, iron, copper, lead, and manganese in the tissues, with particularly high concentrations of aluminum and iron. The biochemical findings revealed redox imbalance and increased levels of oxidized biomolecules, whereas the histological analyses showed inflammatory infiltrates, cellular degeneration, and other morphofunctional alterations consistent with chronic exposure to environmental contaminants. Together, these results indicate that P. geoffroanus exhibits sensitive physiological and tissue- level responses to metal contamination, reinforcing its potential as an environmental bioindicator and highlighting the importance of continued ecotoxicological monitoring in the Paraopeba River. Keywords: bioaccumulation; oxidative stress; environmental bioindicators; histopathology.
Description
Citation
COSTA, Mariana Silva Rabelo. Caracterização bioquímica e histológica do fígado, rim e pulmão de Phrynops geoffroanus (Schweigger, 1812) capturados na Bacia do Paraopeba (Minas Gerais). 2025. 35 f. Trabalho de Conclusão de Curso (Graduação) - Ciências Biológicas – Universidade Federal de Viçosa, Viçosa. 2025.
