Gel mobility shift scanning of pectin-inducible promoter from Penicillium griseoroseum reveals the involvement of a CCAAT element in the expression of a polygalacturonase gene
dc.contributor.author | Ribon, Andréa de O.B. | |
dc.contributor.author | Ribeiro, João Batista | |
dc.contributor.author | Gonçalves, Daniel B. | |
dc.contributor.author | Queiroz, Marisa V. de | |
dc.contributor.author | Araújo, Elza F. de | |
dc.date.accessioned | 2017-10-31T13:20:07Z | |
dc.date.available | 2017-10-31T13:20:07Z | |
dc.date.issued | 2008-07-25 | |
dc.description.abstract | Previous reports have described pgg2, a polygalacturonase-encoding gene of Penicillium griseoroseum, as an attractive model for transcriptional regulation studies, due to its high expression throughout several in vitro growth conditions, even in the presence of non-inducing sugars such as sucrose. A search for regulatory motifs in the 5' upstream regulatory sequence of pgg2 identified a putative CCAAT box that could justify this expression profile. This element, located 270 bp upstream of the translational start codon, was tested as binding target for regulatory proteins. Analysis of a 170 bp promoter fragment by electrophoretic mobility shift assay (EMSA) with nuclear extracts prepared from mycelia grown in pectin-containing culture medium revealed a high mobility complex that was subsequently confirmed by analyzing it with a double-stranded oligonucleotide spanning the CCAAT motif. A substitution in the core sequence for GTAGG partially abolished the formation of specific complexes, showing the involvement of the CCAAT box in the regulation of the polygalacturonase gene studied. | en |
dc.format | pt-BR | |
dc.identifier.issn | 1678-4685 | |
dc.identifier.uri | http://dx.doi.org/10.1590/S1415-47572009005000021 | |
dc.identifier.uri | http://www.locus.ufv.br/handle/123456789/12619 | |
dc.language.iso | eng | pt-BR |
dc.publisher | Genetics and Molecular Biology | pt-BR |
dc.relation.ispartofseries | 32(1) p.129-132, Jan.-Mar. 2009 | pt-BR |
dc.rights | Open Access | pt-BR |
dc.subject | Penicillium griseoroseum | pt-BR |
dc.subject | Polygalacturonase | pt-BR |
dc.subject | 5' upstream regulatory sequence | pt-BR |
dc.subject | Electrophoretic mobility shift assay | pt-BR |
dc.title | Gel mobility shift scanning of pectin-inducible promoter from Penicillium griseoroseum reveals the involvement of a CCAAT element in the expression of a polygalacturonase gene | en |
dc.type | Artigo | pt-BR |