Development and validation of SSR markers for Coffea arabica L.
| dc.contributor.author | Missio, Robson Fernando | |
| dc.contributor.author | Caixeta, Eveline Teixeira | |
| dc.contributor.author | Zambolim, Eunize Maciel | |
| dc.contributor.author | Zambolim, Laércio | |
| dc.contributor.author | Sakiyama, Ney Sussumu | |
| dc.date.accessioned | 2026-09-11T18:25:47Z | |
| dc.date.issued | 2009 | |
| dc.description.abstract | With the objective of developing new SSR markers for Coffea arabica, two enriched genomic libraries with probes (GT)15 and (AGG)10 were constructed. A total of 835 clones were sequenced and 756 presented good quality sequences. Redundant sequences were observed for 113 clones (14.94%). SSRs were found in 287 clones (38%). An estimated size of 417.5Kb of the C. arabica genome was sampled, with an average of one SSR per 1.46Kb. Dinucleotide repeats were more frequent than trinucleotides. Four repeat sequences, (AG/CT)n’ (AC/GT)n’ (AAG/CTT)n’ and (AGG/CCT)n represented 61.1% of the total observed. A total of 96 SSR primers were designed and tested by PCR for two C. arabica genotypes. Ninety new SSR markers were validated for further genetic studies of C. arabica. | en |
| dc.identifier.citation | MISSIO, Robson Fernando. et al. Development and validation of SSR markers for Coffea arabica L. Revista Crop Breeding and Applied Biotechnology, Viçosa, v. 9, n. 4, p. 361-371, 2009. | |
| dc.identifier.issn | 1984-7033 | |
| dc.identifier.uri | https://locus.ufv.br/handle/123456789/35825 | |
| dc.language.iso | eng | |
| dc.publisher | Crop Breeding and Applied Biotechnology | |
| dc.relation.ispartofseries | v. 9 ; n. 4 | |
| dc.rights | Creative Commons Attribution License | |
| dc.subject | SSR marker | en |
| dc.subject | Enriched genomic library | en |
| dc.subject | Coffee | en |
| dc.subject | Molecular marker | en |
| dc.title | Development and validation of SSR markers for Coffea arabica L. | en |
| dc.type | Artigo |