Debaryomyces hansenii UFV-1 intracellular α-Galactosidase characterization and comparative studies with the extracellular enzyme
dc.contributor.author | Rezende, Sebastião T. de | |
dc.contributor.author | Viana, Pollyanna A. | |
dc.contributor.author | Passos, Flávia Maria Lopes | |
dc.contributor.author | Oliveira, Jamil S. | |
dc.contributor.author | Teixeira, Kádima N. | |
dc.contributor.author | Santos, Alexandre M. C. | |
dc.contributor.author | Bemquerer, Marcelo P. | |
dc.contributor.author | Rosa, José C. | |
dc.contributor.author | Santoro, Marcelo M. | |
dc.contributor.author | Guimarães, Valéria M. | |
dc.date.accessioned | 2018-05-29T10:24:11Z | |
dc.date.available | 2018-05-29T10:24:11Z | |
dc.date.issued | 2009-02-18 | |
dc.description.abstract | Debaryomyces hansenii cells cultivated on galactose produced extracellular and intracellular α-galactosidases, which showed 54.5 and 54.8 kDa molecular mass (MALDI-TOF), 60 and 61 kDa (SDS−PAGE) and 5.15 and 4.15 pI values, respectively. The extracellular and intracellular deglycosylated forms presented 36 and 40 kDa molecular mass, with 40 and 34% carbohydrate content, respectively. The N-terminal sequences of the α-galactosidases were identical. Intracellular α-galactosidase showed smaller thermostability when compared to the extracellular enzyme. D. hansenii UFV-1 extracellular α-galactosidase presented higher kcat than the intracellular enzyme (7.16 vs 3.29 s^−1, respectively) for the p-nitrophenyl-α-d-galactopyranoside substrate. The Km for hydrolysis of pNPαGal, melibiose, stachyose, and raffinose were 0.32, 2.12, 10.8, and 32.8 mM, respectively. The intracellular enzyme was acompetitively inhibited by galactose (Ki = 0.70 mM), and it was inactivated by Cu(II) and Ag(I). Enzyme incubation with soy milk for 6 h at 55 °C reduced stachyose and raffinose amounts by 100 and 73%, respectively. | en |
dc.format | pt-BR | |
dc.identifier.uri | http://dx.doi.org/10.1021/jf8030919 | |
dc.identifier.uri | 15205118 | |
dc.identifier.uri | http://www.locus.ufv.br/handle/123456789/19852 | |
dc.language.iso | eng | pt-BR |
dc.publisher | Journal of Agricultural and Food Chemistry | pt-BR |
dc.relation.ispartofseries | v. 57, n. 06, p. 2515–2522, Fevereiro 2009 | pt-BR |
dc.rights | American Chemical Society | pt-BR |
dc.subject | Characterization | pt-BR |
dc.subject | Debaryomyces hansenii UFV-1 | pt-BR |
dc.subject | Deglycosylation | pt-BR |
dc.subject | Galacto-oligosaccharides | pt-BR |
dc.subject | α-Galactosidases | pt-BR |
dc.title | Debaryomyces hansenii UFV-1 intracellular α-Galactosidase characterization and comparative studies with the extracellular enzyme | en |
dc.type | Artigo | pt-BR |
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