Veterinária
URI permanente desta comunidadehttps://locus.ufv.br/handle/123456789/11842
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Resultados da Pesquisa
Item Nematicide activity of microfungi (Orbiliales, Orbiliaceae) after transit through gastrointenstinal tract of “Gallus gallus domesticus”(Revista Brasileira de Saúde e Produção Animal, 2017-01) Silva, Manoel Eduardo da; Silveira, Wendeo Ferreira da; Braga, Fábio Ribeiro; Araújo, Jackson Victor deParasites are common in intensive or organics systems destined for chickens, which is more conducive to the emergence of gastrointestinal parasites, favored by direct contact with soil and other organisms. The growing demand for animal protein stimulates an expansion of production systems, increasing the stocking density. Outdoor poultry breeding systems (organic or not) that enable lower population density and higher animal welfare does not exclude these animals the presence of environmental pathogens. The control of gastrointestinal helminthosis in non-organic intensive and extensive systems is accomplished by administering anthelmintics with high cost and results unsatisfactory due to the misuse of drugs with consequent selection parasite strains resistant to chemical bases. This problem stimulate research into alternative control measures. Nematophagous fungi are used by its enzymatic action in controlled conditions and how environmental biocontrolers of larvae of gastrointestinal nematodes of livestock. This study evaluated the capacity of conidia/chlamydospores of nematophagous fungi as Duddingtonia flagrans (AC001 and CG722) and Monacrosporium thaumasium (NF34A) for cross the gastrointestinal tract of domestic chickens (Gallus gallus domesticus), and yours germination after traffic and predatory activity “in vitro” on larvae of Panagrellus spp. Fungi conidia/chlamydospores was identified in feces of chickens at times of 6, 12 and 24 hours after administration and spores viability was found after observing the germination, mycelial growth, followed by production of traps, capture and death of Panagrellus spp larvae in feces. Fungi Nematophagous are alternative control measures, efficient and innovative technology for the biological control of helminth parasites of chickens.Item Control of bovine gastrointestinal nematode parasites using pellets of the nematode-trapping fungus Monacrosporium thaumasium(Ciência Rural, 2004-03) Araújo, Jackson Victor de; Guimarães, Marcos Pezzi; Campos, Artur Kanadani; Sá, Nilo Chaves de; Sarti, Priscilla; Assis, Rafaela Carolina LopesThe viability of a formulation of the fungus Monacrosporium thaumasium associated with ivermectin was evaluated for the biological control of bovine gastrointestinal nematode parasites. Four groups of five calves each were placed in pastures with a stocking rate of 1.6 animal/hectare. In group 1 (control), the calves did not receive any treatment. In group 2, each animal received 20g of pellets of M. thaumasium orally twice a week during a six-month period that began with the onset of the rainy season (October 23, 2000). In group 3, each animal received 20g of pellets of M. thaumasium orally twice a week during the same period as 2, as well as two strategic treatments with ivermectin (200 mcg/kg) on May 10, 2001 and July 5, 2001. In group 4, the animals were treated with ivermectin alone as described for group 3. EPG counts for group 1 were significantly greater (P< 0.01) than those for groups 2 and 3 and the difference at the end of the study period was near 100%. The EPGs of group 4 animals remained high until the first strategic treatment with ivermectin. Values for groups 1 and 4 differed significantly (P< 0.05) from those of groups 2 and 3 from December 2000 onwards. It was concluded that the use of this dose and periodicity of application of M. thaumasium pellets makes the application of anthelminthic treatments unnecessary.Item Fungi predatory activity on embryonated Toxocara canis eggs inoculated in domestic chickens (Gallus gallus domesticus) and destruction of second stage larvae(Parasitology Research, 2015-09) Araújo, Jackson Victor de; Fonseca, Leandro Abreu da; Hiura, Emy; Lopes, Aline del Carmen Garcia; Paz, Jeanne Saraiva da; Gava, Maylla Garschagen; Flecher, Mayra Cunha; Colares, Manuela; Soares, Filippe Elias de Freitas; Lacerda, Tracy; Braga, Fabio RibeiroThe objective of this study was to evaluate the infectivity of Toxocara canis eggs after interacting with isolated nematophagous fungi of the species Duddingtonia flagrans (AC001) and Pochonia chlamydosporia (VC4), and test the predatory activity of the isolated AC001 on T. canis second stage larvae after 7 days of interaction. In assay A, 5000 embryonated T. canis eggs previously in contact with the AC001 and VC4 isolated for 10 days were inoculated into domestic chickens (Gallus gallus domesticus), and then these animals were necropsied to collect material (digested liver, intestine, muscles and lungs) at 3-, 7-, 14-, and 21-day intervals after inoculation. In assay A, the results demonstrated that the prior interaction of the eggs with isolated AC001 and VC4 decreases the amount of larvae found in the collected organs. Difference (p < 0.01) was observed in the medium larvae counts recovered from liver, lung, intestine, and muscle of animals in the treated groups when compared to the animals in the control group. At the end of assay A, a percentage reduction of 87.1 % (AC001) and 84.5 % (VC4) respectively was recorded. In the result of assay B, the isolated AC001 showed differences (p < 0.01) compared to the control group, with a reduction of 53.4 % in the recovery of L2. Through these results, it is justified to mention that prior interaction of embryonated T. canis eggs with the tested fungal isolates were efficient in reducing the development and migration of this parasite, in addition to the first report of proven predatory activity on L2.Item Reduction of bovine strongilides in naturally contaminated pastures in the southeast region of Brazil(Experimental Parasitology, 2018-11) Oliveira, Isabela de Castro; Vieira, Ítalo Stoupa; Carvalho, Lorendane Millena de; Campos, Artur Kanadani; Freitas, Samuel Galvão; Araujo, Juliana Milani de; Braga, Fábio Ribeiro; Araújo, Jackson Victor deBiological control through the use of nematophagous fungi is a sustainable alternative for combatting helminthes in domestic animals and allows a reduction in the use of anthelmintics. The objective of this research was to evaluate the efficacy of the Arthrobotrys cladodes var macroides fungus in a pelleted formulation, based on sodium alginate and administered twice a week orally, as an alternative for the biological control of nematodes in field-grown young cattle. The experiment was conducted in a farm located in the municipality of Viçosa, MG, where 12 cattle, seven to nine months old, were allocated in two groups (treated group and control group) and distributed in pickets of Brachiaria decumbens, naturally infested with nematode larvae. The animals in the treated group received 1g of sodium alginate matrix pellets for every 10 kg of animal live weight, containing the nematophagous fungus Arthrobotrys cladodes var macroides and administered twice a week in conjunction with commercial feed. In the control group, each animal received 1 g of pellets for every 10 kg of animal live weight, without fungal mycelium added to the feed. Samples of feces and pastures were collected fortnightly for 12 months. The results showed that the most prevalent nematode genera in the coprocultures were Haemonchus sp., Cooperia sp. and Oesophagostomum sp., reflecting the results found in forage. The pasture that contained the animals that received feed with the fungus presented a reduction of 59% and 52% of larvae recovered at distances of 20 cm and 40 cm from the fecal pats, respectively. The mean number of eggs per gram of feces each month and animal body weight did not differ (p > 0.05) between the treated and control groups. Stool and soil samples from both groups were colonized by A. cladodes fungus and other fungi. Administration of Arthrobotrys cladodes var macroides mycelium by means of a sodium alginate matrix twice weekly reduced larval infestation of the surrounding pasture, indicating that this fungus may be a promising biological control of infecting forms of nematodes present in the environment.Item Using the fungus Arthrobotrys cladodes var. macroides as a sustainable strategy to reduce numbers of infective larvae of bovine gastrointestinal parasitic nematodes(Journal of Invertebrate Pathology, 2018-10) Oliveira, Isabela de Castro; Carvalho, Lorendane Millena de; Vieira, Ítalo Stoupa; Campos, Artur Kanadani; Freitas, Samuel Galvão; Araujo, Juliana Milani de; Braga, Fábio Ribeiro; Araújo, Jackson Victor deResearch in the area of sanitation in ruminant production has focused on discovery of potential agents for biological control of helminths with nematophagous fungi and has provided evidence of success. The antagonistic potential of the fungus Arthrobotrys cladodes var. macroides on infective larvae of bovine gastrointestinal parasitic nematodes was evaluated by scanning electron microscopy. Additionally, an in vivo test of the resistance to digestive processes and viability of the fungus was carried out using a formulation based on sodium alginate administered orally in cattle. Production of conidia and chlamydospores was high. In in vitro tests, the number of infective nematode larvae was reduced 68.7% by the fungus in the treated group compared to the control group. The interaction between the fungus and the nematodes was confirmed by scanning electron microscopy. Plates containing fecal samples collected after oral administration of 100 g of pellets containing the A. cladodes fungus showed that the fungus survived passage through the gastrointestinal tract of ruminants, grew on agar, formed traps and preyed on L3 larvae of gastrointestinal parasites. The results of the present study provide a new opportunity for alternative, environmentally safe control of ruminant nematodes.Item Activity of the nematophagous fungi Pochonia chlamydosporia, Duddingtonia flagrans and Monacrosporium thaumasium on egg capsules of Dipylidium caninum(Veterinary Parasitology, 2009-12-03) Araujo, Juliana Milani; Araújo, Jackson Victor de; Braga, Fabio Ribeiro; Carvalho, Rogério Oliva; Ferreira, Sebastião RodrigoNematophagous fungi are potential biological control agents of helminths. The in vitro ovicidal effect of four isolates of the nematophagous fungi Pochonia chlamydosporia (VC1 and VC4), Duddingtonia flagrans (AC001) and Monacrosporium thaumasium (NF34) was evaluated on egg capsules of Dipylidium caninum, a cestode parasite of dogs, cats and humans. One thousand egg capsules of D. caninum were plated on 2% water-agar with the grown isolates and control without fungus. The ovicidal activity of these fungi was evaluated 5, 10 and 15 days after incubation. Only P. chlamydosporia showed ovicidal activity (p < 0.05) on D. caninum egg capsules, of 19.6% (VC1) and 20% (VC4) on the 5th day; 44.2% (VC1) and 31.5% (VC4) on the 10th day; and 49.2% (VC1) and 41.9% (VC4) on the 15th day. D. flagrans and M. thaumasium caused no morphological damage to egg capsules. The results demonstrated that P. chlamydosporia was in vitro effective against capsules and eggs of D. caninum, and can be considered as a potential biological control agent for this helminth.Item Biological control of Ascaris suum eggs by Pochonia chlamydosporia fungus(Veterinary Research Communications, 2011-07-28) Ferreira, Sebastião Rodrigo; Araújo, Jackson Victor de; Braga, Fábio Ribeiro; Araujo, Juliana Milani; Frassy, Luiza Neme; Ferreira, Aloízio SoaresAscaris suum is a gastrointestinal nematode parasite of swines. The aim of this study was to observe Pochonia chlamydosporia fungus on biological control of A. suum eggs after fungus passage through swines gastrointestinal tract. Eighteen pigs, previously dewormed, were randomly divided into three groups: group 1, treated with the fungus isolate VC4; group 2, treated with the fungus isolate VC1 and group 3 did not receive fungus (control). In the treated groups, each animal received a 9 g single dose of mycelium mass containing P. chlamydosporia (VC1 or VC4). Thereafter, animal fecal samples were collected at the following intervals: 8, 12, 24, 36, 48, 72 and 96 h after treatment beginning and these were poured in Petri dishes containing 2% water-agar culture medium. Then, 1,000 A. suum eggs were poured into each dish and kept in an incubator at 26°C and in the dark for 30 days. After this period, approximately 100 eggs were removed from each Petri dish and morphologically analyzed under light microscopy following the ovicidal activity parameters. The higher percentage observed for isolated VC4 eggs destruction was 57.5% (36 h) after fungus administration and for isolate VC1 this percentage was 45.8% (24 h and 72 h) (p > 0.01). P. chlamydosporia remained viable after passing through the gastrointestinal tract of swines, maintaining its ability of destroying A. suum eggs.Item Coadministration of sodium alginate pellets containing the fungi Duddingtonia flagrans and Monacrosporium thaumasium on cyathostomin infective larvae after passing through the gastrointestinal tract of horses(Research in Veterinary Science, 2012-11-22) Tavela, Alexandre de Oliveira; Araújo, Jackson Victor de; Braga, Fábio Ribeiro; Silveira, Wendeo Ferreira da; Silva, Vinicius Herold Dornelas e; Carretta Júnior, Moacir; Borges, Luana Alcântara; Araujo, Juliana Milani; Benjamin, Laércio dos Anjos; Carvalho, Giovanni Ribeiro; Paula, Alessandra Teixeira deThe predatory nematophagous fungi have been used as an alternative control of gastrointestinal nematodes of domestic animals in natural and laboratory conditions. However, it is unclear if the association of some of these species could bring some kind of advantage, from a biological standpoint. In this context, this study consisted of two tests in vitro: in assay A, the assessment of the viability of the association of pellets in sodium alginate matrix containing the fungus Duddingtonia flagrans (AC001) and Monacrosporium thaumasium (NF34) and its predatory activity on infective larvae (L3) of cyathostomin after passing through the gastrointestinal tract of horses and assay B, assessment of the cyathostomin L3 reduction percentage in coprocultures. Twelve crossbred horses, females, with a mean weight of 356 kg and previously dewormed were divided in three groups with four animals each: group 1, each animal received 50 g of pellets containing mycelial mass of the fungus D. flagrans and 50 g of pellets of the fungus M. thaumasium, associated and in a single oral dose; group 2, 100 g of pellets containing D. flagrans and 100 g of pellets containing M. thaumasium, associated and in a single oral dose; group 3, control. Faecal samples were collected from animals in the treated and control groups at time intervals of 12, 24, 36, 48, 60 and 72 h after the administration of treatments and placed in Petri dishes containing 2% water-agar (assay A) and cups for coprocultures (assay B). Subsequently, 1000 cyathostomin L3 were added to each Petri dish (assay A) and 1000 cyathostomin eggs were added to each coproculture (assay B) of fungi-treated and control groups. At the end of 15 days, there was observed that the two associations of pellets containing the fungi tested showed predatory activity after passing through the gastrointestinal tract of horses (assay A). In assay B, all the intervals studied showed reduction rate in the number of L3 recovered from coprocultures exceeding 80%. However, no difference (p > 0.01) was seen in recovery of not predated L3 between the fungi-treated groups in the time intervals studied. The results obtained showed that the associations of pellets (50 or 100 g of each fungal isolate) were viable after passage through the gastrointestinal tract in horses and could be used in natural conditions.Item Duddingtonia flagrans formulated in rice bran in the control of Oesophagostomum spp. intestinal parasite of swine(Experimental Parasitology, 2017-11-10) Rodrigues, Joao Victor Facchini; Braga, Fabio Ribeiro; Campos, Artur Kanadani; Carvalho, Lorendane Millena de; Araujo, Juliana Milani; Aguiar, Anderson Rocha; Ferraz, Carolina Magri; Silveira, Wendeo Ferreira da; Valadao, Marisa Caixeta; Oliveira, Thais de; Freitas, Samuel Galvao de; Araújo, Jackson Victor deThree experimental assays with Duddingtonia flagrans (isolated AC001) were carried out. The growth of the genus Duddingtonia present in formulation of rice bran, its predatory capability on Oesophagostomum spp. infective larvae (L3) in petri dishes (assay 1), its action in faecal cultures with eggs of that parasite (assay 2) and isolate's capability of predation after passing through gastrointestinal tract of swine (assay 3) was evaluated. At assay 3, feces were collected at time intervals of 12, 24, 36, 48, and 60 h after feed animals with the formulation. Assays 1 and 2 showed a statistical difference (p < 0.01) by the F test when comparing the treated group with the control group. At the both assays, was observed in the treated group a reduction percentage of 74.18% and 88.38%, respectively. In assay 3, there was a statistical difference between the treated group and the control group at all collection times (p < 0.01). Regarding the collection periods, there was no statistical difference over time in the treatment group (p > 0.05). The results demonstrate that the fungal isolate AC001 formulated in rice bran can prey on L3 of Oesophagostomum spp., in vitro and after passing through the gastrointestinal tract, without loss of viability. This isolate may be an alternative in the control of Oesophagostomum spp. in swine.Item Nematophagous fungi for biological control of gastrointestinal nematodes in domestic animals(Applied Microbiology and Biotechnology, 2013-11-22) Braga, Fabio Ribeiro; Araújo, Jackson Victor deSeveral studies have been conducted using fungi in the biological control of domestic animals and humans. In this respect, a large amount of research has been undertaken to understand the particularities of each fungus used. These fungi have been demonstrated to act on all classes of helminthes. Therefore, they should not only be called nematophagous but also helmintophagous. Evidence of enzymatic action has also revealed their mechanism of action, as well as potential metabolites that could be synthesized as bioactive molecules. Cultural barriers to the use of fungi should be broken down, since the impact on the environment is minimal. In this context, much is already known about the mechanism of interaction of these organisms with their ‘targets’. Recent research has pointed to the search for substances derived from nematophagous fungi that have demonstrated their ovicidal and/or larvicidal activity, thus being a global premise to be studied further. Crude extracts derived from nematophagous fungi of predator and ovicidal groups reduce the amount of larvae of gastrointestinal nematodes and prevent the hatching of their eggs, since they have been demonstrated to act with extracellular proteases and other enzymes. Furthermore, the activity of these enzymes has begun to be explored regarding their possible interaction with the exoskeleton of arthropods, which could emerge as an alternative method of tick control. Finally, it should be clear that nematophagous fungi in general are ‘old friends’ that are ready to the ‘fight with our old enemies’, the gastrointestinal helminth parasites harmful to human and animal health.