Centro de Ciências Exatas e Tecnológicas
URI permanente desta comunidadehttps://locus.ufv.br/handle/123456789/9791
Navegar
2 resultados
Resultados da Pesquisa
Item ATP - bioluminescence as a technique to evaluate the microbiological quality of water in food industry(Brazilian Archives of Biology and Technology, 2000-07) Costa, Patrícia Dolabela; Andrade, Nélio José de; Passos, Frederico José Vieira; Brandão, Sebastião César Cardoso; Rodrigues, Carolina Gonçalves FreireATP-bioluminescence was used to evaluate the microbiological quality of water samples collected from the water supply, the water treatment system and from a dairy plant, including ammonia-cooling water and industrial water. For industrial water, there was relation between the ATP-bioluminescence technique and microbial count. There were no differences (p>0.05) between water supply and ammonia-cooling water samples for total and free ATP concentrations nor for the microbial counts. Different microbial ATP concentrations were found for these water samples. The results suggested that the physical chemical quality of ammonia cooling water decreased the RLU measurements slightly. It could be concluded that the total ATP concentration was the most effective technique to evaluate the microbiological quality of water used in the food indsutry by ATP-bioluminescence.Item Interference of some organic substances and microorganisms adhered to stainless steel in ATP-bioluminescence measurement(Brazilian Archives of Biology and Technology, 2008-05) Simm, Erny Marcelo; Andrade, Nélio José de; Mendonça, Regina Célia Santos; Passos, Frederico José Vieira; Chaves, José Benício PaesAdhesion of organic substances and microorganisms on stainless steel surface was evaluated. The log RLU measurement was affected at a lower or higher degree, by type or concentration of the organic substances (casein, lipid, sucrose and their combinations) adhered to surface. However, if the samples analyzed were from the food processing surfaces, they would have been considered to be under satisfactory hygienic conditions with log RLU < 2.18 (< 150 RLU). The log RLU measurements of Staphylococcus carnosus-adhered surfaces, combined with or without the organic substances, were higher (p<0.05) than those of the Bacillus subtilis spores-adhered surface, regardless of the concentration. The samples taken from the surfaces adhered with the casein, lipid, sucrose and 2.9x101 DMC.cm-2 and 2.9x102 DMC.ml-1 did not have any significant differences (p≥0.05) on ATP-bioluminescence measurements. However, the surfaces adhered with 2.9x103 DMC.cm-2 of B. subtilis spores and organic substances showed an increase in the log RLU measurement compared to surfaces containing only the organic substances.