Artigos
URI permanente para esta coleçãohttps://locus.ufv.br/handle/123456789/11852
Navegar
4 resultados
Resultados da Pesquisa
Item Destruction of Anoplocephala perfoliata eggs by the nematophagous fungus Pochonia chlamydosporia(Journal of Equine Veterinary Science, 2010-12) Silva, André R.; Araújo, Jackson V.; Braga, Fábio R.; Alves, Camila D. F.; Ribeiro Filho, José DantasThe in vitro effect of an isolate of the nematophagous fungus Pochonia chlamydosporia (VC1) on the eggs of Anoplocephala perfoliata was evaluated. The eggs were morphologically analyzed for their integrity using light microscopy (10× objectives), plated on 9.0-cm diameter petri dishes containing 2% WA culture medium with and without fungal isolate (control), grown for 10 days, and 10 replicates were prepared per group. In all, 1000 eggs of A perfoliata were plated on petri dishes containing 2% water agar culture medium with (VC1) and without the fungal isolate (control). After 3, 5, 7, and 10 days, approximately 100 eggs were removed from each plate and classified on the basis of the following parameters: without alteration; type 1, lytic effect without morphological damage to eggshell; type 2, lytic effect with morphological alteration of embryo and eggshell; and type 3, lytic effect with morphological alteration of embryo and eggshell, in addition to hyphal penetration and internal egg colonization and destruction. The P chlamydosporia fungus demonstrated ovicidal activity (P < .05) on the eggs of A perfoliata in the studied intervals presenting type 3 effects of 35%, 42.5%, 53.83%, and 71.17% for the intervals 3, 5, 7, and 10 days, respectively. P chlamydosporia is a potential biological control agent for the eggs of A perfoliata.Item Ovicidal activity of seven Pochonia chlamydosporia fungal isolates on Ascaris suum eggs(Tropical Animal Health and Production, 2010-11-19) Ferreira, Sebastião R.; Araújo, Jackson V.; Braga, Fabio R.; Araujo, Juliana M.; Carvalho, Rogério O.; Silva, André R.; Frassy, Luiza N.; Freitas, Leandro G.The ovicidal effect of the nematophagous fungus Pochonia chlamydosporia on eggs of Ascaris suum was tested under laboratory conditions. A. suum eggs were plated on 2% water–agar with seven fungal isolates (Isol. 5, Isol. 31, Isol. 1, VC1, Isol. 12, Isol. 22 and VC4) and control without fungus. After 5, 7, 10, 14, 15 and 21 days of incubation, approximately 100 eggs were removed from the plates and classified according to the following parameters: type 1, biochemical and physiological effect without morphological damage to the eggshell, type 2, lytic effect with morphological alteration of the eggshell and embryo and type 3, lytic effect with morphological alteration of eggshell and embryo showing hyphal penetration and internal egg colonization. The isolates effectively destroyed A. suum eggs and all types of effects were observed during the experiment. There was no variation in ovicidal capacity (type 3 effect) among the isolates (p > 0.05) throughout the experiment. After 21 days, isolate 5 showed the highest percentages of type 3 effect (58.33%). The results indicated that P. chlamydosporia (Isol. 5, Isol. 31, Isol. 1, VC1, Isol. 12, Isol. 22 and VC4) can destroy A. suum eggs and is, therefore, a potential biological control agent of nematodes.Item Predatory activity of the nematophagous fungus Duddingtonia flagrans on horse cyathostomin infective larvae(Tropical Animal Health and Production, 2010-03-07) Braga, Fabio R.; Araújo, Jackson V.; Silva, André. R.; Carvalho, Rogério O.; Araujo, Juliana M.; Ferreira, Sebastião R.; Benjamin, Laércio A.This work was performed to determine the predatory capacity in vitro of the nematophagous fungus Duddingtonia flagrans (isolate AC001) on cyathostomin infective larvae of horse (L3). The experimental assay was carried out on plates with 2% water-agar (2% WA). In the treated group, each plate contained 1.000 L3 and 1.000 conidia of the fungus. The control group without fungus only contained 1.000 L3 in the plates. Ten random fields (4 mm diameter) were examined per plate of treated and control groups, every 24 h for seven days under an optical microscope (10× and 40× objective lens) for non-predated L3 counts. After 7 days, the non-predated L3 were recovered from the Petri dishes using the Baermann method. The interaction there was a significant reduction (p < 0.01) of 93.64% in the cyathostomin L3 recovered. The results showed that the D. flagrans is a potential candidate to the biological control of horse cyathostomin L3.Item Survival of Pochonia chlamydosporia in the gastrointestinal tract of experimentally treated dogs(Research in Veterinary Science, 2011-10-20) Araujo, Juliana M.; Araújo, Jackson V.; Braga, Fabio R.; Araújo, Dayane M.; Ferreira, Sebastião R.; Soares, Filippe E.F.; Benjamin, Laércio dos A.The predatory capacity of the nematophagous fungus Pochonia chlamydosporia (isolate VC4) after passage through the gastrointestinal tract of dogs was assessed in vivo against Toxocara canis eggs. Twelve dogs previously wormed were divided into two groups of six animals and caged. The treatments consisted of a fungus-treated group (VC4) and a control group without fungus. Each dog of the fungus-treated group received a single 4 g dose of mycelial mass of P. chlamydosporia (VC4). Fecal samples from animals of both groups (treated and control) were collected at five different times (6, 12, 24, 36, and 48 h) after fungal administration, and placed in Petri dishes. Each Petri dish of both groups for each studied time interval received approximately 1000 T. canis eggs. Thirty days after the fecal samples were collected, approximately one hundred eggs were removed from each Petri dish of each studied time interval and evaluated by light microscopy (LM) and scanning electron microscopy (SEM). Microscopy examination of plates inoculated with the fungus showed that the isolate VC4 was able to destroy the T. canis eggs with destruction percentages of 28.6% (6 h), 29.1% (12 h), 32.0% (24 h), 31.7% (36 h), and 37.2% (48 h). These results suggest that P. chlamydosporia can be used as a tool for the biological control of T. canis eggs in feces of contaminated dogs.