Agroecological coffee management increases arbuscular mycorrhizal fungi diversity
Arquivos
Data
2019
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Editor
Plos One
Resumo
Agroecology aims to maintain ecosystem services by minimizing the impact of agriculture and promoting the use of biological potential. Arbuscular mycorrhizal fungi (AMF) are elements which are key to improving crop productivity and soil quality. It is pertinent to understand how agricultural management in the tropics affects the AMF spatio-temporal community composition, especially in crops of global importance, such as coffee (Coffea
arabica L.). Soil and root samples were collected from three localities under three management systems (agroecological, conventional and forest fragment), during the phenological
stages of coffee (flowering, grain filling, harvesting). Spores were extracted for morphological identification and molecular community analysis by PCR–DGGE. Dendrograms were
prepared and the bands were sequenced and analyzed by bioinformatics. No differences were observed in the richness of morphospecies between management systems, localities
and period, but little is known about tropical species. Molecular analysis showed that the agroecological management system was similar to natural forest and with a higher diversity
indices than conventional management. Locality and period of sample affect AMF community composition. It is necessary to associate classical taxonomic evaluations with molecular
biological techniques because different approaches can lead to different outcomes. This study contributes to the understanding of the impact of agriculture management systems on
AMF and provides evidence that agroecology is a management system applicable to sustainable coffee production.
Descrição
Palavras-chave
Agroecological coffee, Mycorrhizal fungi