Microbiologia
URI permanente desta comunidadehttps://locus.ufv.br/handle/123456789/11840
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Item Cloning and characterization of a gene encoding the endopolygalacturonase of Penicillium griseoroseum(Biotechnology Letters, 1999-05) Ribon, A. O. B.; Coelho, J. L. C.; Barros, E. G. de; Araújo, E. F.A conserved region of a polygalacturonase (PG) gene from Penicillium griseoroseum was PCR amplified and used to screen a genomic library from this fungus. The nucleotide sequence of the isolated clone (pggI) consisted of 1497 bp, including a coding region of 1251 bp. This region potentially encodes a protein of 376 amino acids, and is interrupted by two introns. Extensive homology was observed between this protein and several fungal endopolygalacturonases. DNA hybridization analyses revealed that there is a low copy number of pggI in the P. griseoroseum genome, probably one or two copies.Item Differential expression of polygalacturonase-encoding genes from Penicillium griseoroseum in different carbon sources(Journal of Industrial Microbiology and Biotechnology, 2002-06-14) Ribon, A. O. B.; Queiroz, M. V.; Coelho, J. L. C.; Araújo, E. F deA second polygalacturonase - encoding gene ( pgg2 ) of Penicillium griseoroseum was cloned and consists of an opening reading frame of 1107 bp after removal of two introns. The gene encodes a protein of 369 amino acids with a predicted molecular mass of 38.3 kDa. The deduced protein sequence exhibited high homology with other fungal endopolygalacturonases. A polymerase chain reaction ( PCR ) - based strategy was used to study the expression patterns of pgg1 and pgg2 genes under different culture conditions and our results show that both genes are regulated by the carbon source at the transcriptional level. The pgg1 transcript was detected at 76 h of fungal growth in pectin while the pgg2 transcript was also induced by sucrose. The addition of yeast extract to glucose medium abolished the repressive effect of glucose, suggesting that the transcription of these genes is controlled by different mechanisms.Item Production of pectin lyase by Penicillium griseoroseum as a function of the inoculum and culture conditions(World Journal of Microbiology and Biotechnology, 1992-10-09) Brumano, M. H. N.; Coelho, J. L. C.; Araújo, E. F.; Silva, D. O.Optimum activity of an extracellular pectin lyase produced by Penicillium griseoroseum in submerged culture was after 120 h using 0.1% (w/v) citrus pectin as substrate. Sucrose at 0.1% (w/v) stimulated enzyme production and citrus pectin gave the highest activity of enzyme per unit growth.