Microbiologia

URI permanente desta comunidadehttps://locus.ufv.br/handle/123456789/11840

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2012 - 20132

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Autor: search.filters.author.Araújo, Elza Fernandes deData inicial: 2012Data final: 2013Assunto: search.filters.subject.IRAP

Resultados da Pesquisa

Agora exibindo 1 - 2 de 2
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    Development of molecular markers based on retrotransposons for the analysis of genetic variability in Moniliophthora perniciosa
    (European Journal of Plant Pathology, 2012-11) Santana, Mateus Ferreira; Araújo, Elza Fernandes de; Queiroz, Marisa Vieira de; Souza, Jorge Teodoro de; Mizubuti, Eduardo Seiti Gomide
    Moniliophthora perniciosa is a fungus that causes witches’ broom disease (WBD) in the cacao tree (Theobroma cacao). The M. perniciosa genome contains different transposable elements; this prompted an evaluation of the use of its retrotransposons as molecular markers for population studies. The inter-retrotransposon amplified polymorphism (IRAP) and retrotransposon-microsatellite amplified polymorphism (REMAP) techniques were used to study the variability of 70 M. perniciosa isolates from different geographic origins and biotypes. A total of 43 loci was amplified. Cluster analysis of different geographical regions of C biotype revealed two large groups in the state of Bahia, Brazil. Techniques using retrotransposon-based molecular markers showed advantages over previously used molecular techniques for the study of genetic variability in M. perniciosa.
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    Terminal repeat retrotransposons as DNA markers in fungi
    (Journal of Basic Microbiology, 2013-02-26) Santana, Mateus Ferreira; Batista, Aline Duarte; Ribeiro, Lílian Emídio; Araújo, Elza Fernandes de; Queiroz, Marisa Vieira de
    In this study, we demonstrate that ClIRAP primers designed using the transposable element RetroCl1 sequence from Colletotrichum lindemuthianum can be used to generate an efficient IRAP (inter‐retrotransposon amplified polymorphism) molecular marker to study intra‐ and inter‐species diversity in fungi. It has been previously demonstrated that primers generated from this TRIM‐like element can be used in the Colletotrichum species. We now prove that the RetroCl1 sequence can also be used to analyze diversity in different fungi. IRAP profiles were successfully generated for 27 fungi species from 11 different orders, and intra‐species genetic variability was detected in six species. The ClIRAP primers facilitate the use of the IRAP technique for a variety of fungi without prior knowledge of the genome.