Fitopatologia - Artigos

URI permanente para esta coleçãohttps://locus.ufv.br/handle/123456789/11741

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    Leaf blight and defoliation of Eugenia spp. caused by Cylindrocladium candelabrum and C. spathiphylli in Brazil
    (Summa Phytopathologica, 2010-11-06) Alfenas, Rafael Ferreira; Alfenas, Acelino Couto; Poltronieri, Luiz Sebastião; Verzignassi, Jaqueline Rosemeire; Benchimol, Ruth Linda; Poltronieri, Tathianne Pastana de Sousa
    Leaf blight and defoliation of Eugenia stipitata Mc Vaugh and Eugenia patrisii Vahl, caused respectively by Cylindrocladium candelabrum (Calonectria scoparia) and C. spathiphylli (Calonectria spathiphylli) are reported in the state of Pará, Brazil. On both host species, the disease is characterized by dark brown lesions of different sizes and shapes. A whitish bright sporulation, resembling Cylindrocladium is observed on the necrotic lesions by using a stereomycroscope or a pocket lense (10-20 X). Under favorable conditions and depending on the level of infection, intense premature tree defoliation may also be found.Although the conidial germination and mycelial growth were higher at 25ºC for both species, C. candelabrum was more sensitive to the variation of temperature (10, 20, 30 and 40 ºC) than C. spathiphylli. This is the first report of C. candelabrum and C. spathiphylli on Eugenia stipitata (araçá-boi) and on Eugenia patrisii (ubaia-da-amazônia), respectively in Brazil.
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    Development of microsatellite markers for the guava rust fungus, Puccinia psidii
    (Molecular Ecology Resources, 2008-03) Zhong, Shaobin; Yang, Baojun; Alfenas, Acelino Couto
    We developed and characterized 15 polymorphic microsatellite markers present in the genome of the guava rust fungus, Puccinia psidii. The primers for these microsatellite markers were designed by sequencing clones from a genomic DNA library enriched for a simple sequence repeat (SSR) motif of (AG). All these 15 primer pairs successfully amplified DNA fragments from a sample of 22 P. psidii isolates, revealing a total of 71 alleles. The observed heterozygosity at the 15 loci ranged from 0.05 to 1.00. The SSR markers developed would be useful for population genetics study of the rust fungus.