Fisiologia Vegetal
URI permanente para esta coleçãohttps://locus.ufv.br/handle/123456789/185
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Resultados da Pesquisa
Item Functional analysis of the thioredoxin system during seed germination in Arabidopsis thaliana(Universidade Federal de Viçosa, 2019-03-29) Nascimento, Carolina Pereira; Nesi, Adriano Nunes; http://lattes.cnpq.br/3331610618877369A series of processes occurs during seed formation, including remarkable changes from early development to the end of germination. The changes associated with processes initiated mainly after seed imbibition are usually characterized by extensive changes in redox state of seed reserve proteins and of pivotal enzymes for protein mobilization and usage. Such changes in redox state are often mediated by Thioredoxins (Trxs), which are protein oxiredutases capable of catalyzing the reduction of disulfide bonds in target proteins, thereby regulating their structure and function. Here, we analyzed the previously characterized mutants of NADPH-dependent Trx reductase A and B (ntra ntrb), two independent mutant lines of mitochondrial thioredoxin o1 (trxo1) and two mutant thioredoxin h2 (trxh2) mutant lines. Our results indicate that plants deficient for the NADPH-dependent thioredoxin system are able to mobilize their reserves, but at least partially fail to use these reserves during germination, thereby leading to lower availability of energy substrates than wild type seeds. Trx mutants also show decreased activity of regulatory systems needed to maintain cellular homeostasis. Moreover, we observed reduced respiration in mutant seeds and seedlings, which in parallel with an impaired energy metabolism, disrupts core biological processes responsible for proper germination and early development of Trx mutants. In conclusion, the results suggest that the lack of thioredoxin induces a substantial adaptation in seeds and seedlings, which undergo a metabolic reprogramming to adapt to a new redox state.