Fisiologia Vegetal
URI permanente para esta coleçãohttps://locus.ufv.br/handle/123456789/185
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Resultados da Pesquisa
Item Role of sucrose for tobacco guard cell osmoregulation: osmolyte or substrate?(Universidade Federal de Viçosa, 2013-02-27) Daloso, Danilo de Menezes; Loureiro, Marcelo Ehlers; http://lattes.cnpq.br/0306680503261422A characterization of transgenic tobacco plants overexpressing potato sucrose synthase 3 gene (NtSuSy3) under control of KST1 promoter was performed in order to analyze the role of sucrose metabolism on GC osmoregulation. Also, we performed a metabolic flux analysis in guard cell enriched epidermal fragment (EF) of Nicotiana tabacum in order to investigate changes in GC metabolism during stomatal aperture light-induced. NtSuSy3 plants showed higher stomatal conductance, transpiration rate, whole plant transpiration, and net photosynthetic rate than wild type (WT). Several changes in GC metabolism were observed in transgenic plants are discussed in the text. In different stomatal aperture light-induced experiments, it was observed a decrease in sucrose content, while no changes were detected in the sugar content in the medium; suggesting that the sugars decreased observed is due to breakdown and not efflux of GC. Using a feeding strategy in EF submitted to NaH13C03 followed by LC-qTOF-MS analysis, a 13 C-enrichment in sucrose, malate, fumarate and glutamine were observed. The possible function of these metabolites for GC osmoregulation are discussed in the text. Taken together, the data showed here provide evidence for another role of sucrose for GC osmoregulation. Our data suggest that sucrose breakdown, not just sucrose accumulation, can be performed to induce stomatal opening in tobacco.