Fisiologia Vegetal
URI permanente para esta coleçãohttps://locus.ufv.br/handle/123456789/185
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Resultados da Pesquisa
Item Desmonostoc salinum CCM-UFV059, a novel cyanobacteria from a saline- alkaline lake: molecular and physiological responses to light, desiccation and salt stress(Universidade Federal de Viçosa, 2019-02-22) Alvarenga, Luna Viggiano de; Araújo, Wagner Luiz; http://lattes.cnpq.br/5025552116262277Cyanobacteria are widespread photosynthetic prokaryotes and are among the oldest organisms on Earth. During their long evolution, cyanobacteria developed an enormous diversity in terms of morphology, metabolic plasticity and molecular properties, which seems to be important factors to cope with limiting environmental conditions and allowed their ecological success in almost all known photic ecosystems. The first part of this work consisted of the taxonomic characterization, using the polyphasic approach, of the strain Desmonostoc salinum CCM-UFV059, a filamentous heterocytous cyanobacterium isolated from a hypersaline lake. Taken together, our data allowed the description of a new species and the first strain of the Desmonostoc genus from a saline environment. The second part of this work aimed to decipher the main salt acclimation mechanisms present in Desmonostoc salinum CCM-UFV059, because most studies on cyanobacterial salt acclimation have been carried out on unicellular strains, which cannot fix N 2 . We performed a comparative study using the model strain Nostoc PCC7120, and we could observe a remarkable high salt tolerance displayed by Desmonostoc salinum CCM-UFV059. In cells of Desmonostoc salinum CCM-UFV059 the intracellular sodium content was significantly lower than in Nostoc PCC7120 and these cells were able to sustain photosynthetic activity up to 0.5 M NaCl while Nostoc PCC7120 cells were not. Moreover, Desmonostoc salinum CCM- UFV059 induced sucrose over-accumulation under desiccation stress conditions, which allowed this strain to survive harsh desiccation stress. Together with the presence of highly unsaturated lipids in the membrane, the high sucrose production and the intense export of sodium could explain, at least partially, how Desmonostoc salinum CCM-UFV059 is capable of acclimate to high salinities and to resist longer desiccation periods. Collectively, our results provide the first insights into the physiological adaptations explaining the remarkable high salt and desiccation tolerance presented by this strain. Furthermore, given that cyanobacteria have several biotechnological applications, such as the production of biomass for human and animal consumption, and metabolites of industrial interest, the third part of this work was performed to analyze the physiological and metabolic responses of Desmonostoc salinum CCM-UFV059 submitted to different light availabilities, aiming at finding the light regime suitable for maximal biomass production as well as to better understand how distinct growth conditions may interfere within the basal metabolism. Collectively our results indicate that Desmonostoc salinum CCM-UFV059 display a highly plastic metabolism and the ability to grow in a large range of light regimes, that open the possibility to outdoor cultivation and commercial use of this species that has a great biotechnological potential. Notwithstanding, further research is clearly required in order to enable a large scale cultivation of Desmonostoc salinum CCM-UFV059.