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URI permanente para esta coleçãohttps://locus.ufv.br/handle/123456789/11847

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2006 - 200912010 - 20131

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Autor: search.filters.author.Moreira, Maurilio AlvesAssunto: search.filters.subject.Phaseolus vulgaris

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    Differentially expressed proteins during an incompatible interaction between common bean and the fungus Pseudocercospora griseola
    (Molecular Breeding, 2013-07-30) Borges, Leandro Luiz; Santana, Fernanda Abreu; Castro, Isabel Samila Lima; Arruda, Klever Márcio Antunes; Ramos, Humberto Josué de Oliveira; Moreira, Maurilio Alves; Barros, Everaldo Gonçalves de
    The common bean (Phaseolus vulgaris L.) is the main source of protein and an important source of minerals in several countries around the world. Angular leaf spot, caused by the fungus Pseudocercospora griseola, is one of the major diseases of the common bean. In this work, we used two-dimensional gel electrophoresis and mass spectrometry to analyze alterations in the proteome of common bean leaves challenged with an incompatible race of P. griseola. Twenty-three differentially expressed proteins were detected in leaves of cultivar AND 277 collected at 12, 24 and 48 h after inoculation. The proteins were digested with trypsin and submitted to MALDI-TOF/TOF and MicrOTOF-Q electrospray mass spectrometry. Nineteen of them were identified upon MS/MS fragmentation. Most of these proteins are involved with amino acid metabolism, terpenoid metabolism, phenylpropanoid biosynthesis, antioxidant systems, vitamin and cofactor metabolism, plant–pathogen interaction, carbohydrate metabolism, photosynthesis, or genetic information processing, showing that the interaction in this pathosystem affects different genes from various metabolic pathways and processes.
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    Biochemical composition and indigestible oligosaccharides in Phaseolus vulgaris L. seeds
    (Plant Foods for Human Nutrition, 2006-06-20) Fialho, Lílian da Silva; Guimarães, Valéria Monteze; Barros, Everaldo Gonçalves de; Moreira, Maurilio Alves; Dias, Luiz Antônio dos Santos; Oliveira, Maria Goreti de Almeida; José, Inês Chamel; Rezende, Sebastião Tavares de
    Common beans have a high nutritional value, but contain galactooligosaccharides (GO), which cause flatulence and intestinal discomfort in humans. The biochemical composition of ten bean cultivars was determined to select those of high protein and low GO contents. The cultivars varied in carbohydrate (47.02–60.17%), GO (3.12 – 5.71%), protein (22.17–33.50%), lipid (1.13–1.81%), moisture (11.42–12.93%) and ash contents (4.08–5.61%). ‘Mexico 222’ presented the highest α-galactosidase activity. Protein and GO contents were positively correlated. ‘Perry Marrow’ combined high protein and low GO concentrations, indicating it can be used in improvement programs aiming at high-quality cultivars for human consumption.