Artigos
URI permanente para esta coleçãohttps://locus.ufv.br/handle/123456789/11847
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Item Development of a method to quantify sucrose in soybean grains(Food Chemistry, 2012-02-15) Teixeira, Arlindo I.; Ribeiro, Lucas F.; Rezende, Sebastião T.; Barros, Everaldo G.; Moreira, Maurílio A.The sucrose content of soybean seeds affects the final flavor of soy-derived products. The aim of this work was to develop a simple, low-cost, spectrophotometric method for sucrose quantification in soybean seeds. To achieve this goal, we combined the action of invertase, an enzyme that hydrolyses sucrose into fructose and glucose, with glucose oxidase, an enzyme widely used for glucose quantification. This system was adapted to ELISA plates, making large-scale analyses possible at low cost, with potential application in routine analyses. To validate this method, sucrose content was determined in seeds of 14 soybean cultivars by this new method, as well as by HPLC and the enzymatic method of Stitt. The correlation coefficients were high and significant between the results obtained with the new method and the HPLC method (r = 0.9766) and the Stiff method (0.9461).Item Differential expression of four soybean bZIP genes during Phakopsora pachyrhizi infection(Functional & Integrative Genomics, 2015-05-27) Alves, Murilo S.; Soares, Zamira G.; Vidigal, Pedro M. P.; Barros, Everaldo G.; Poddanosqui, Adriana M. P.; Aoyagi, Luciano N.; Abdelnoor, Ricardo V.; Marcelino-Guimarães, Francismar C.; Fietto, Luciano G.Asian soybean rust (ASR), caused by the obligate biotrophic fungus Phakopsora pachyrhizi, is one of most important diseases in the soybean (Glycine max (L.) Merr.) agribusiness. The identification and characterization of genes related to plant defense responses to fungal infection are essential to develop ASR-resistant plants. In this work, we describe four soybean genes, GmbZIP62, GmbZIP105, GmbZIPE1, and GmbZIPE2, which encode transcription factors containing a basic leucine zipper (bZIP) domain from two divergent classes, and that are responsive to P. pachyrhizi infection. Molecular phylogenetic analyses demonstrated that these genes encode proteins similar to bZIP factors responsive to pathogens. Yeast transactivation assays showed that only GmbZIP62 has strong transactivation activity in yeast. In addition, three of the bZIP transcription factors analyzed were also differentially expressed by plant defense hormones, and all were differentially expressed by fungal attack, indicating that these proteins might participate in response to ASR infection. The results suggested that these bZIP proteins are part of the plant defense response to P. pachyrhizi infection, by regulating the gene expression related to ASR infection responses. These bZIP genes are potential targets to obtain new soybean genotypes resistant to ASR.