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URI permanente para esta coleçãohttps://locus.ufv.br/handle/123456789/11847

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20132

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Autor: search.filters.author.Almeida, Maíra N. deData inicial: 2010Data final: 2019Tem arquivos: SimAssunto: search.filters.subject.Ethanol

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Agora exibindo 1 - 2 de 2
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    Optimization of Endoglucanase and Xylanase activities from fusarium verticillioides for simultaneous saccharification and fermentation of sugarcane bagasse
    (Applied Biochemistry and Biotechnology, 2013-10-30) Almeida, Maíra N. de; Guimarães, Valéria M.; Falkoski, Daniel L.; Paes, Guilherme B. T.; Ribeiro Jr., José Ivo; Visser, Evan M.; Alfenas, Rafael F.; Pereira, Olinto L.; Rezende, Sebastião T. de
    Enzymatic hydrolysis is an important but expensive step in the production of ethanol from biomass. Thus, the production of efficient enzymatic cocktails is of great interest for this biotechnological application. The production of endoglucanase and xylanase activites from F. verticillioides were optimized in a factorial design (25) followed by a CCDR design. Endoglucanase and xylanase activities increased from 2.8 to 8.0 U/mL and from 13.4 to 114 U/mL, respectively. The optimal pH and temperature were determined for endoglucanase (5.6, 80 °C), cellobiase (5.6, 60 °C), FPase (6.0, 55 °C) and xylanase (7.0, 50 °C). The optimized crude extract was applied in saccharification and fermentation of sugarcane bagasse from which 9.7 g/L of ethanol was produced at an ethanol/biomass yield of 0.19.
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    Direct ethanol production from glucose, xylose and sugarcane bagasse by the corn endophytic fungi Fusarium verticillioides and Acremonium zeae
    (Journal of Biotechnology, 2013-07-31) Almeida, Maíra N. de; Guimarães, Valéria M.; Falkoski, Daniel L.; Visser, Evan M.; Siqueira, Germano A.; Milagres, Adriane M.F.; Rezende, Sebastião T. de
    Production of ethanol with two corn endophytic fungi, Fusarium verticillioides and Acremonium zeae, was studied. The yield of ethanol from glucose, xylose and a mixture of both sugars were 0.47, 0.46 and 0.50 g/g ethanol/sugar for F. verticillioides and 0.37, 0.39 and 0.48 g/g ethanol/sugar for A. zeae. Both fungi were able to co-ferment glucose and xylose. Ethanol production from 40 g/L of pre-treated sugarcane bagasse was 4.6 and 3.9 g/L for F. verticillioides and A. zeae, respectively, yielding 0.31 g/g of ethanol per consumed sugar. Both fungi studied were capable of co-fermenting glucose and xylose at high yields. Moreover, they were able to produce ethanol directly from lignocellulosic biomass, demonstrating to be suitable microorganisms for consolidated bioprocessing.