Purification and characterization of Aspergillus terreus α-Galactosidases and their use for hydrolysis of Soymilk Oligosaccharides
dc.contributor.author | Ferreira, Joana Gasperazzo | |
dc.contributor.author | Reis, Angélica Pataro | |
dc.contributor.author | Guimarães, Valéria Monteze | |
dc.contributor.author | Falkoski, Daniel Luciano | |
dc.contributor.author | Silva Fialho, Lílian da | |
dc.contributor.author | Rezende, Sebastião Tavares de | |
dc.date.accessioned | 2018-04-04T10:02:13Z | |
dc.date.available | 2018-04-04T10:02:13Z | |
dc.date.issued | 2011-02-18 | |
dc.description.abstract | α-Galactosidases has the potential to hydrolyze α-1-6 linkages in raffinose family oligosaccharides (RFO). Aspergillus terreus cells cultivated on wheat bran produced three extracellular forms of α-galactosidases (E1, E2, and E3). E1 and E2 α-galactosidases presented maximal activities at pH 5, while E3 α-galactosidase was more active at pH 5.5. The E1 and E2 enzymes showed stability for 6 h at pH 4–7. Maximal activities were determined at 60, 55, and 50°C, for E1, E2, and E3 α-galactosidase, respectively. E2 α-galactosidase retained 90% of its initial activity after 70 h at 50°C. The enzymes hydrolyzed ρNPGal, melibiose, raffinose and stachyose, and E1 and E2 enzymes were able to hydrolyze guar gum and locust bean gum substrates. E1 and E3 α-galactosidases were completely inhibited by Hg2+, Ag+, and Cu2+. The treatment of RFO present in soy milk with the enzymes showed that E1 α-galactosidase reduced the stachyose content to zero after 12 h of reaction, while E2 promoted total hydrolysis of raffinose. The complete removal of the oligosaccharides in soy milk could be reached by synergistic action of both enzymes | en |
dc.format | pt-BR | |
dc.identifier.issn | 15590291 | |
dc.identifier.uri | https://doi.org/10.1007/s12010-011-9198-y | |
dc.identifier.uri | http://www.locus.ufv.br/handle/123456789/18612 | |
dc.language.iso | eng | pt-BR |
dc.publisher | Applied Biochemistry and Biotechnology | pt-BR |
dc.relation.ispartofseries | v. 164, Issue 7, p. 1111–1125, August 2011 | pt-BR |
dc.rights | Springer Science+Business Media, LLC | pt-BR |
dc.subject | α-Galactosidase | pt-BR |
dc.subject | Aspergillus terreus | pt-BR |
dc.subject | Raffinose | pt-BR |
dc.subject | Stachyose | pt-BR |
dc.subject | Soybean | pt-BR |
dc.subject | Anti-nutritional factors | pt-BR |
dc.title | Purification and characterization of Aspergillus terreus α-Galactosidases and their use for hydrolysis of Soymilk Oligosaccharides | en |
dc.type | Artigo | pt-BR |
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