Veterinária
URI permanente desta comunidadehttps://locus.ufv.br/handle/123456789/11842
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Item In vitro evaluation of the action of the nematophagous fungi Duddingtonia flagrans, Monacrosporium sinense and Pochonia chlamydosporia on Fasciola hepatica eggs(World Journal of Microbiology and Biotechnology, 2008-01-06) Braga, F. R.; Araújo, J. V.; Campos, A. K.; Araújo, J. M.; Carvalho, R. O.; Silva, A. R.; Tavela, A. O.This work evaluated the in vitro action of four isolates of the nematophagous fungi Duddingtonia flagrans (AC001), Monacrosporium sinense (SF53) and Pochonia chlamydosporia (VC1 and VC4) on eggs of Fasciola hepatica. The eggs were plated on 2% water-agar with the grown isolates and control without fungus. After 7, 14 and 21 days, the eggs were removed and classified according to the following parameters: effect type 1, lytic effect with no morphological damage to eggshells; type 2, lytic effect with morphological changes in eggshells and embryos; and type 3, lytic effect with morphological changes in embryos and eggshells, with hyphal penetration and internal egg colonization. Pochonia chlamydosporia showed ovicidal activity on F. hepatica eggs in the studied intervals of the type-3 effect, of 12.8% (VC1) and 16.5% (VC4); 14.4% (VC1) and 18.7% (VC4), 20.1% (VC1) and 21.5 % (VC4), over 7, 14 and 21 days respectively. No statistical difference was found (P > 0.01) among the isolates VC1 and VC4 for effects type 1, 2 and 3 during the studied intervals. Duddingtonia flagrans (AC001) and Monacrosporium sinense fungi only showed effect type 1, with no significant difference between them, with the following results: 60.1% (AC001) and 57.5% (SF53); 62.3% (AC001) and 62.0% (SF53); 66.5% (AC001) and 73.4% (SF53), over 7, 14 and 21 days respectively. Pochonia chlamydosporia fungi negatively influenced the in vitro F. hepatica viability. Therefore it can be considered as a potential biological control agent for this helminth.Item Predation of Ancylostoma spp. dog infective larvae by nematophagous fungi in different conidial concentrations(Veterinary Parasitology, 2009-05-12) Maciel, A. S.; Araújo, J. V.; Campos, A. K.; Lopes, E. A.; Freitas, L. G.In the present work, it was evaluated the in vitro effect of 12 isolates from the fungal species Arthrobotrys, Duddingtonia, Nematoctonus and Monacrosporium genera in different conidial concentrations on the capture of Ancylostoma spp. dog infective larvae (L3), on 2% water-agar medium at 25 °C, at the end of a period of 7 days. The concentrations used for each nematophagous fungus were 1000, 5000, 10,000, 15,000 and 20,000 conidia/Petri dish plated with 1000 Ancylostoma spp. L3. All nematode-trapping fungi isolates tested reduced the averages of the uncaptured Ancylostoma spp. L3 recovered, with the increase of the fungal inoculum concentration, in comparison to the fungus-free control (p < 0.05). The adhesive network producing species were better predators than the constricting ring or adhesive knob producing species. Duddingtonia flagrans (Isolate CG768) was the most effective, reducing the averages of the uncaptured Ancylostoma spp. L3 recovered in 92.8%, 96.3%, 97.5%, 98.3% and 98.9%, respectively in five fungal inoculum concentrations established. Other effective nematophagous fungi were Arthrobotrys robusta (Isolate I31), which reduced the averages of the uncaptured Ancylostoma spp. L3 recovered in 85.4%, 88.3%, 90.7%, 92.5% and 95.2%, and Arthrobotrys oligospora (Isolate A183), with reductions of 66.6%, 79.8%, 86.8%, 89.5% and 90.8%, respectively for both, in the five fungal inoculum concentrations established. No difference was found between Isolates A183 and I31 in the conidial concentrations of 15,000/Petri dish. Nematoctonus robustus (Isolate D1) and Arthrobotrys bronchophaga (Isolate AB) had the smallest percentages of reduction among the tested isolates and showed the lowest predacious activity. The Isolates CG768, I31 and A183 were considered potential biological control agents of Ancylostoma spp. dog free-living stages, being directly influenced by the fungal inoculum concentration.Item Ovicidal effect of nematophagous fungi on Taenia taeniaeformis eggs(World Journal of Microbiology and Biotechnology, 2009-03) Braga, F. R.; Araújo, J. V.; Carvalho, R. O.; Silva, A. R.; Araujo, J. M.; Tavela, A. O.; Costa, P. R. S.; Campos, A. K.This work evaluated the ovicidal effect of the nematophagous fungi Monacrosporium sinense (SF53), Monacrosporium thaumasium (NF34) and Pochonia chlamydosporia (VC1) on Taenia taeniaeformis eggs in laboratory conditions. T. taeniaeformis eggs were plated on 2% water-agar with the grown isolates and control without fungus and examined at seven and fourteen days post-inoculation. At the end of the experiment, P. chlamydosporia showed ovicidal activity (P < 0.01) on T. taeniaeformis eggs unlike the other two species, mainly for internal egg colonization with percentage results of 32.2–54.0% at 7th and 14th day, respectively. The other fungi only showed lytic effect without morphological damage to eggshell. Results demonstrated that P. chlamydosporia was in vitro effective against Taenia taeniaeformis eggs unlike the other fungi. In this way, the use of P. chlamydosporia is suggested as a potential biological control agent for eggs of this cestode.