Microbiologia

URI permanente desta comunidadehttps://locus.ufv.br/handle/123456789/11840

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1998 - 20002

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Data inicial: 1992Assunto: search.filters.subject.Aspergillus nidulans

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Agora exibindo 1 - 2 de 2
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    Electrophoretic characterization ofaspergillus nidulans strains with chromosomal duplications
    (Genetics and Molecular Biology, 2000-06) Queiroz, Marisa V. de; Pizzirani-Kleiner, Aline Aparecida; Azevedo, João Lúcio
    Pulsed-field gel electrophoresis was used to characterize strains ofAspergillus nidulans with a chromosomal duplication Dp(I-II). Morphologically deteriorated and improved variants of these strains were also analyzed. The electrophoretic karyotype demonstrated that in two duplicated strains (A and B) the 4.2 Mb band, which corresponds to chromosome II, was absent and a new band was observed. Hybridization studies using the uapA (chromosome I) and wA (chromosome II) genes demonstrated that the new band corresponded to chromosome II plus the duplicated segment of chromosome I. The size of the chromosomal duplication was approximately 1.0 Mb. Analysis of the chromosomal bands of a morphologically improved strain showed that the duplicated segment of chromosome I was completely lost. The morphologically deteriorated variants V9 and V17 had the same karyotype as the duplicated strains. However, the deteriorated variant V5 lost part of chromosome I and had a rearrangement involving chromosome V. This rearrangement may have resulted from the mutagenic treatment used to obtain the genetic markers. Pulsed-field gel electrophoresis was found to be an excellent tool for locating chromosomal rearrangements.
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    Characterization of an Aspergillus nidulans mutant with abnormal distribution of nuclei in hyphae, metulae, phialides and conidia
    (FEMS Microbiology Letters, 1998-07-10) Queiroz, Marisa Vieira de; Azevedo, João Lúcio de
    The V10 deteriorated variant of Aspergillus nidulans has hyphae, metulae, phialides and conidia with abnormal nuclear distributions. The alterations observed were: increase in the number of nuclei in hyphae, metulae and phialides, presence of anucleate, uninucleate and multinucleate conidia, abnormal vegetative growth and defective conidiation. When 0.5 M NaCl was added to the medium, an increase in the number of conidia was observed but their morphology and number of nuclei were not modified. The gene responsible for these alterations was named anuA1. The anuA1 gene is located on linkage group VII and is possibly involved in nuclear migration to hyphae, metulae, phialides and conidia. z 1998 Federation of European Microbiological Societies