Microbiologia

URI permanente desta comunidadehttps://locus.ufv.br/handle/123456789/11840

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Data inicial: 2001Data final: 2007Assunto: search.filters.subject.Polygalacturonase

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Agora exibindo 1 - 3 de 3
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    Structural organization of polygalacturonase-encoding genes from Penicillium griseoroseum
    (Genetics and Molecular Biology, 2002) Ribon, Andréa O. B.; Queiroz, Marisa V.; Araújo, Elza F. de
    The pectinolytic system of Penicillium griseoroseum has been studied as a model to investigate aspects of gene organization in filamentous fungi. Here we show that the endopolygalacturonase-coding genes previously isolated exist as single copies in the fungus genome. DNA blot analysis revealed the presence of corresponding genes in other Penicillium species, although only one or two genes were found in opposition to the endoPG gene family reported for other filamentous fungi. The nucleotide and amino acid sequences of Penicillium PG genes of retrieved from data banks were compared for intron length and number, codon usage, and consensus sequences for translation initiation sites. The introns are conserved in the same position, although there was no conservation of their nucleotide sequences. Other sequence features resemble those seen in Aspergillus and Neurospora genes
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    Molecular characterization and evaluation of pectinase and cellulase production of Penicillium spp.
    (Biotechnology Letters, 2002-05) Pereira, Jorge Fernando; Queiroz, Marisa Vieira de; Gomes, Eliane Aparecida; Muro-Abad, Júpiter Israel; Araújo, Elza Fernandes de
    Penicillium species were analyzed with molecular markers and for pectinase and cellulase production. RAPD and PCR-RFLP analysis indicated high polymorphism among at least 5 of 10 Penicillium species. Five species were chosen for pectinase and cellulase production in liquid medium and four of which appeared similar based on molecular analyses. P. brevicompactum and P. griseoroseum gave the highest pectinase production and were highly divergent by molecular techniques.
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    Differential expression of polygalacturonase-encoding genes from Penicillium griseoroseum in different carbon sources
    (Journal of Industrial Microbiology and Biotechnology, 2002-06-14) Ribon, A. O. B.; Queiroz, M. V.; Coelho, J. L. C.; Araújo, E. F de
    A second polygalacturonase - encoding gene ( pgg2 ) of Penicillium griseoroseum was cloned and consists of an opening reading frame of 1107 bp after removal of two introns. The gene encodes a protein of 369 amino acids with a predicted molecular mass of 38.3 kDa. The deduced protein sequence exhibited high homology with other fungal endopolygalacturonases. A polymerase chain reaction ( PCR ) - based strategy was used to study the expression patterns of pgg1 and pgg2 genes under different culture conditions and our results show that both genes are regulated by the carbon source at the transcriptional level. The pgg1 transcript was detected at 76 h of fungal growth in pectin while the pgg2 transcript was also induced by sucrose. The addition of yeast extract to glucose medium abolished the repressive effect of glucose, suggesting that the transcription of these genes is controlled by different mechanisms.