Microbiologia

URI permanente desta comunidadehttps://locus.ufv.br/handle/123456789/11840

Navegar

Filtros

2002 - 20072

Configurações

Autor: search.filters.author.Queiroz, Marisa Vieira deData inicial: 2002Data final: 2009Assunto: search.filters.subject.Penicillium

Resultados da Pesquisa

Agora exibindo 1 - 2 de 2
  • Imagem de Miniatura
    Item
    Isolation of recombinant strains with enhanced pectinase production by protoplast fusion between Penicillium expansum and Penicillium griseoroseum
    (Brazilian Journal of Microbiology, 2007-01) Queiroz, Marisa Vieira de; Lana, Taís Guimarães; Gonçalves, Daniel Bonoto; Araújo, Elza Fernandes de; Brito, Admilson Toscano Ribeiro de; Varavallo, Maurilio Antonio
    Protoplast fusion between complementary auxotrophic and morphological mutant strains of Penicillium griseoroseum and P. expansum was induced by polyethylene glycol and calcium ions (Ca2+). Fusant strains were obtained in minimal medium and a prototrophic strain, possibly diploid, was chosen for haplodization with the fungicide benomyl. Different recombinant strains were isolated and characterized for occurrence of auxotrophic mutations and pectinolytic enzyme production. The fusant prototrophic did not present higher pectinase production than the parental strains, but among 29 recombinants analyzed, four presented enhanced enzyme activities. The recombinant RGE27, which possesses the same auxotrophic and morphologic mutations as the P. griseoroseum parental strain, presented a considerable increase in polygalacturonase (3-fold) and pectin lyase production (1.2-fold).
  • Imagem de Miniatura
    Item
    Molecular characterization and evaluation of pectinase and cellulase production of Penicillium spp.
    (Biotechnology Letters, 2002-05) Pereira, Jorge Fernando; Queiroz, Marisa Vieira de; Gomes, Eliane Aparecida; Muro-Abad, Júpiter Israel; Araújo, Elza Fernandes de
    Penicillium species were analyzed with molecular markers and for pectinase and cellulase production. RAPD and PCR-RFLP analysis indicated high polymorphism among at least 5 of 10 Penicillium species. Five species were chosen for pectinase and cellulase production in liquid medium and four of which appeared similar based on molecular analyses. P. brevicompactum and P. griseoroseum gave the highest pectinase production and were highly divergent by molecular techniques.