Microbiologia

URI permanente desta comunidadehttps://locus.ufv.br/handle/123456789/11840

Navegar

Filtros

2011 - 20152

Configurações

Autor: search.filters.author.Araújo, E.F. deData inicial: 2010Data final: 2015

Resultados da Pesquisa

Agora exibindo 1 - 2 de 2
  • Imagem de Miniatura
    Item
    Improved pectinase production in Penicillium griseoroseum recombinant strains
    (Journal of Applied Microbiology, 2011-06-27) Teixeira, J.A.; Gonçalves, D.B.; Queiroz, M.V. de; Araújo, E.F. de
    To obtain recombinant strains of Penicillium griseoroseum that produce high levels of pectin lyase (PL) and polygalacturonase (PG) simultaneously. A strain with high production of PL was transformed with the plasmid pAN52pgg2, containing the gene encoding PG of P. griseoroseum, under control of the gpd promoter gene from Aspergillus nidulans. Southern blot analysis demonstrated that all strain had at least one copy of pAN52pgg2 integrated into the genome. The recombinant strain P. griseoroseum T20 produced levels of PL and PG that were 266‐ and 27‐fold greater, respectively, than the wild‐type strain. Furthermore, the extracellular protein profile of recombinant T20 showed two protein bands of c. 36 and 38 kDa, associated with PL and PG, respectively. This recombinant strain T20 produces PL and PG using carbon sources of low costs, and an enzyme preparation that is free of cellulolytic and proteolytic activities. PL and PG play an important role in the degradation of pectin. Owing to their use in the juice and wines industries, there is a growing interest in the inexpensive production of these enzymes. This work describes an efficient system of protein expression and secretion using the fungus P. griseoroseum.
  • Imagem de Miniatura
    Item
    High genetic variability in endophytic fungi from the genus Diaporthe isolated from common bean (Phaseolus vulgaris L.) in Brazil
    (Journal of Applied Microbiology, 2015-11-06) Santos, T.T. dos; Souza Leite, T. de; Queiroz, C.B. de; Araújo, E.F. de; Pereira, O.L.; Queiroz, M.V. de
    The goals of the present study were to identify, to analyse the phylogenetic relations and to evaluate the genetic variability in Diaporthe endophytic isolates from common bean. Diaporthe sp., D. infecunda and D. phaseolorum strains were identified using multilocus phylogeny (rDNA ITS region; EF1‐α, β‐tubulin, and calmodulin genes). IRAP (Inter‐Retrotransposon Amplified Polymorphism) and REMAP (Retrotransposon‐Microsatellite Amplified Polymorphism) molecular markers reveal the existence of high genetic variability, especially among D. infecunda isolates. It was concluded that the multilocus phylogenetic approach was more effective than individual analysis of ITS sequences, in identifying the isolates to species level, and that IRAP and REMAP markers can be used for studying the genetic variability in the genus Diaporthe particularly at the intraspecific level. The combined use of molecular tools such as multilocus phylogenetic approach and molecular markers, as performed in this study, is the best way to distinguish endophytic strains of Diaporthe isolated from common bean (Phaseolus vulgaris L.).