Bioquímica e Biologia Molecular

URI permanente desta comunidadehttps://locus.ufv.br/handle/123456789/11837

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Resultados da Pesquisa

Agora exibindo 1 - 6 de 6
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    Treatment of soy milk with Debaryomyces hansenii cells immobilised in alginate
    (Food Chemistry, 2009-05-15) Souza Júnior, Waldeck Campanha de; Rezende, Sebastião Tavares de; Viana, Pollyanna Amaral; Falkoski, Daniel Luciano; Reis, Angélica Pataro; Machado, Solimar Gonçalves; Barros, Everaldo Gonçalves de; Guimarães, Valéria Monteze
    Whole cells of Debaryomyces hansenii UFV-1 were permeabilised with ethanol and immobilised in calcium alginate hydrogel. The optimum pH and temperature for α-galactosidase activities of permeabilised free (PFC) and permeabilised immobilised cells (PIC) were 4.5 and 60 °C; and 4.0 and 70 °C, respectively. PIC α-galactosidase was more stable than that of PFC. The incubation of PIC at 60 and 70 °C promoted an increase in α-galactosidase activity. The α-galactosidase activity was maintained when PIC was used in three repeated batches. The Km values for PIC and PFC α-galactosidases, with ρNPαGal, were 0.82 mM and 0.30 mM, respectively. Soy milk treatment with PIC for 6 h at 60 °C promoted 100% hydrolysis of raffinose oligosaccharides.
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    Extracellular α-Galactosidase from Debaryomyces hansenii UFV-1 and Its use in the hydrolysis of raffinose oligosaccharides
    (Journal of Agricultural and Food Chemistry, 2006-02-17) Rezende, Sebastião T. de; Marques, Virgínia M.; Trevizano, Larissa M.; Passos, Flávia M. L.; Oliveira, Maria G. A.; Bemquerer, Marcelo P.; Oliveira, Jamil S.; Guimarães, Valéria M.; Viana, Pollyanna A.
    Raffinose oligosaccharides (RO) are the factors primarily responsible for flatulence upon ingestion of soybean-derived products. ROs are hydrolyzed by α-galactosidases that cleave α-1,6-linkages of α-galactoside residues. The objectives of this study were the purification and characterization of extracellular α-galactosidase from Debaryomyces hansenii UFV-1. The enzyme purified by gel filtration and anion exchange chromatographies presented an Mr value of 60 kDa and the N-terminal amino acid sequence YENGLNLVPQMGWN. The Km values for hydrolysis of pNPαGal, melibiose, stachyose, and raffinose were 0.30, 2.01, 9.66, and 16 mM, respectively. The α-galactosidase presented absolute specificity for galactose in the α-position, hydrolyzing pNPGal, stachyose, raffinose, melibiose, and polymers. The enzyme was noncompetitively inhibited by galactose (Ki = 2.7 mM) and melibiose (Ki = 1.2 mM). Enzyme treatments of soy milk for 4 h at 60 °C reduced the amounts of stachyose and raffinose by 100%.
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    Spectroscopic and thermodynamic properties of Debaryomyces hansenii UFV-1 α-galactosidases
    (International Journal of Biological Macromolecules, 2010-04-01) Rezende, Sebastião T.; Viana, Pollyanna A.; Meza, Andreia N.; Gomide, Felipe T.F.; Nagem, Ronaldo A.P.; Santos, Alexandre M.C.; Santoro, Marcelo M.; Guimarães, Valéria M.
    Spectroscopic and thermodynamic properties were determined for Debaryomyces hansenii UFV-1 extracellular and intracellular α-galactosidases. α-Galactosidases showed similar secondary structure compositions (α-helix, β-sheet parallel and β-turn). Effects of pH and temperature on the structure of α-galactosidases were investigated using circular dichroism spectroscopy. It was more pronounced at low pH. Microcalorimetry was employed for the determination of thermodynamic parameters. Immediate thermal denaturation reversibility was not observed for α-galactosidases; it occurred as a thermodynamically driven process. Extracellular α-galactosidase, at pH 5.5, showed lower Tm when compared to the intracellular enzyme. The CD and DSC data suggest that D. hansenii α-galactosidases have different behaviors although they possess some similar secondary structures.
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    Debaryomyces hansenii UFV-1 intracellular α-Galactosidase characterization and comparative studies with the extracellular enzyme
    (Journal of Agricultural and Food Chemistry, 2009-02-18) Rezende, Sebastião T. de; Viana, Pollyanna A.; Passos, Flávia Maria Lopes; Oliveira, Jamil S.; Teixeira, Kádima N.; Santos, Alexandre M. C.; Bemquerer, Marcelo P.; Rosa, José C.; Santoro, Marcelo M.; Guimarães, Valéria M.
    Debaryomyces hansenii cells cultivated on galactose produced extracellular and intracellular α-galactosidases, which showed 54.5 and 54.8 kDa molecular mass (MALDI-TOF), 60 and 61 kDa (SDS−PAGE) and 5.15 and 4.15 pI values, respectively. The extracellular and intracellular deglycosylated forms presented 36 and 40 kDa molecular mass, with 40 and 34% carbohydrate content, respectively. The N-terminal sequences of the α-galactosidases were identical. Intracellular α-galactosidase showed smaller thermostability when compared to the extracellular enzyme. D. hansenii UFV-1 extracellular α-galactosidase presented higher kcat than the intracellular enzyme (7.16 vs 3.29 s^−1, respectively) for the p-nitrophenyl-α-d-galactopyranoside substrate. The Km for hydrolysis of pNPαGal, melibiose, stachyose, and raffinose were 0.32, 2.12, 10.8, and 32.8 mM, respectively. The intracellular enzyme was acompetitively inhibited by galactose (Ki = 0.70 mM), and it was inactivated by Cu(II) and Ag(I). Enzyme incubation with soy milk for 6 h at 55 °C reduced stachyose and raffinose amounts by 100 and 73%, respectively.
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    Activity of Debaryomyces hansenii UFV-1 a-galactosidases against a-D-galactopyranoside derivatives
    (Carbohydrate Research, 2011-04-01) Rezende, Sebastião T. de; Viana, Pollyanna A.; Alves, Arianne de A.; Manfrini, Rozângela M.; Alves, Ricardo J.; Bemquerer, Marcelo P.; Santoro, Marcelo M.; Guimarães, Valéria M.
    α-d-Galactopyranosides were synthesized and their inhibitory activities toward the Debaryomyces hansenii UFV-1 extracellular and intracellular α-galactosidases were evaluated. Methyl α-d-galactopyranoside was the most potent inhibitor compared to the others tested, with values of 0.82 and 1.12 mmol L−1, for extracellular and intracellular enzymes, respectively. These results indicate that the presence of a hydroxyl group in the C-6 position of α-d-galactopyranoside derivatives is important for the recognition by D. hansenii UFV-1 α-galactosidases.
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    Hydrolysis of soybean isoflavones by Debaryomyces hansenii UFV-1 immobilised cells and free β-glucosidase
    (Food Chemistry, 2014-03-01) Maitan-Alfenas, Gabriela P.; Lage, Lorena G. de A.; Almeida, Maíra N. de; Visser, Evan M.; Rezende, Sebastião T. de; Guimarães, Valéria M.
    An intracellular β-glucosidase from Debaryomyces hansenii UFV-1 was produced in an YP medium with cellobiose as the carbon source. This enzyme was purified, characterised and presented a Mr of 65.15 kDa. Yeast cells containing the intracellular β-glucosidase were immobilised in calcium alginate. The free β-glucosidase and immobilised cells containing the enzyme presented optima values of pH and temperature of 6.0 and 45 °C and 5.5 and 50 °C, respectively. The free enzyme maintained 62% and 47% of its original activity after 90 days at 4 °C and after 15 days at room temperature, respectively. The immobilisation process resulted in higher enzyme thermostability at 45 and 50 °C. Soy molasses treatment with the free enzyme and the immobilised cells containing β-glucosidase, for 2 h at 40 °C, promoted efficient hydrolysis of isoflavone glicosides to their aglycon forms. The results suggest that this enzyme could be used in the food industry, in the free or immobilised forms, for a safe and efficient process to hydrolyse isoflavone glycosides in soy molasses.