Bioquímica e Biologia Molecular

URI permanente desta comunidadehttps://locus.ufv.br/handle/123456789/11837

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1999 - 199912010 - 20151

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Autor: search.filters.author.Cruz, Cosme DamiãoData final: 2017Assunto: search.filters.subject.Soybean

Resultados da Pesquisa

Agora exibindo 1 - 2 de 2
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    Genetic distances in soybean based on RAPD markers
    (Bragantia, 1999) Corrêa, Ronan Xavier; Abdelnoor, Ricardo Vilela; Faleiro, Fábio Gelape; Cruz, Cosme Damião; Moreira, Maurilio Alves; Barros, Everaldo Gonçalves De
    Four methods were applied to determine pairwise genetic distances among five soybean genotypes which are potential genitors for a mapping population. Additionally, individual plants from the most divergent pair of genotypes were evaluated by the RAPD technique to determine their degree of homozygosity. Genetic distances based on RAPD data were calculated by the modified Rogers' distance, and also by the following arithmetical complements of similarity: simple match, Nei and Li, and Gower. These genetic distances were similar, presenting a correlation coefficient ranging from 0.99 to 1.00. In all four methods lines UFV 91-717 and Ichigowase were the most divergent ones (4.53 to 21.43%). DNA samples from five plants from each of the two most divergent genotypes were amplified with 28 different primers. Among the amplified products, only five were polymorphic in each group (2.10%), demonstrating their high intragroup degree of homozygosity. These homozygosity were maintained when DNA samples from 12 plants from each of the two most divergent genotypes were amplified. These parameters were extremely useful for the confirmation of the chosen pair of genitors to generate a mapping population.
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    Quantification of anti-nutritional factors and their correlations with protein and oil in soybeans
    (Anais da Academia Brasileira de Ciências, 2015-08-31) Bueno, Rafael D.; Borges, Leandro L.; God, Pedro I.V. Good; Piovesan, Newton D.; Teixeira, Arlindo I.; Cruz, Cosme Damião; Barros, Everaldo G. de
    Soybeans contain about 30% carbohydrate, mainly consisting of non-starch polysaccharides (NSP) and oligosaccharides. NSP are not hydrolyzed in the gastrointestinal tract of monogastric animals. These NSP negatively affect the development of these animals, especially the soluble fraction. This work aimed to establish a method to quantify NSP in soybeans, using high performance liquid chromatography (HPLC), and to estimate correlations between NSP, oligosaccharides, protein and oil. Sucrose, raffinose + stachyose, soluble and insoluble NSP contents were determined by HPLC. Oil and protein contents were determined by near-infrared spectroscopy (NIRS). The soluble PNAs content showed no significant correlation with protein, oil, sucrose and raffinose + stachyose contents, but oligosaccharides showed a negative correlation with protein content. These findings open up the possibility of developing cultivars with low soluble NSP content, aiming to develop feed for monogastric animals.