Navegando por Autor "Silva Junior, Abelardo"
Agora exibindo 1 - 3 de 3
- Resultados por Página
- Opções de Ordenação
Item Genetic evaluation of IS900 partial sequence of Mycobacterium avium subsp. paratuberculosis Brazilian isolates from bovine milk(Tropical Animal Health and Production, 2012-03-16) Carvalho, Isabel Azevedo; Silva, Victor Oliveira; Vidigal, Pedro Marcus Pereira; Silva Junior, Abelardo; Moreira, Maria Aparecida ScatamburloMycobacterium avium subsp. paratuberculosis (MAP) is the causative agent of paratuberculosis. Insertion sequence IS900 is used for the identification of MAP. The objective of this study was to verify the genetic conservation of IS900 sequences in raw milk samples. To evaluate genetic conservation, 206 quarter milk samples and 16 bulk-tank milk samples were collected. DNA extraction and IS900 PCR were performed in all samples. Six samples amplified the expected fragment. To confirm the identity of the amplified fragments, PCR products were cloned and sequenced. The resulting sequences were compared with other MAP sequences from GenBank, and it was possible to identify eight polymorphic regions and to form five distinct haplotypes. The number of mutations in each haplotype was verified. IS900 sequence is a very well-conserved sequence that could be used as tool for the molecular detection of this agent and epidemiological purposes. The results showed the first genetic analysis on Brazilian isolates of MAP.Item The paradox of feline cCoronavirus pathogenesis: a review(Advances in Virology, 2011-06-01) Myrrha, Luciana Wanderley; Silva, Fernanda Miquelitto Figueira; Peternelli, Ethel Fernandes de Oliveira; Resende, Maurício; Almeida, Márcia Rogéria de; Silva Junior, AbelardoFeline coronavirus (FCoV) is an enveloped single-stranded RNA virus, of the family Coronaviridae and the order Nidovirales. FCoV is an important pathogen of wild and domestic cats and can cause a mild or apparently symptomless enteric infection, especially in kittens. FCoV is also associated with a lethal, systemic disease known as feline infectious peritonitis (FIP). Although the precise cause of FIP pathogenesis remains unclear, some hypotheses have been suggested. In this review we present results from different FCoV studies and attempt to elucidate existing theories on the pathogenesis of FCoV infection.Item The paradox of feline coronavirus pathogenesis: a review(Advances in Virology, 2011-06-01) Myrrha, Luciana Wanderley; Silva, Fernanda Miquelitto Figueira; Peternelli, Ethel Fernandes de Oliveira; Silva Junior, Abelardo; Resende, Maurício; Márcia Rogéria de AlmeidaFeline coronavirus (FCoV) is an enveloped single-stranded RNA virus, of the family Coronaviridae and the order Nidovirales. FCoV is an important pathogen of wild and domestic cats and can cause a mild or apparently symptomless enteric infection, especially in kittens. FCoV is also associated with a lethal, systemic disease known as feline infectious peritonitis (FIP). Although the precise cause of FIP pathogenesis remains unclear, some hypotheses have been suggested. In this review we present results from different FCoV studies and attempt to elucidate existing theories on the pathogenesis of FCoV infection.