Navegando por Autor "Matta, Sérgio Luis Pinto da"
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Item Aspectos reprodutivos de Dendropsophus minutus (PETERS, 1872) no município de Viçosa, Minas Gerais(Revista Ceres, 2007-05) Oliveira, Eliana Faria de; Feio, Renato Neves; Matta, Sérgio Luis Pinto daA biologia reprodutiva de machos de Dendropsophus minutus foi estudada em Viçosa, Minas Gerais, no período de fevereiro de 2005 a janeiro de 2006. Procurou-se estabelecer o ciclo reprodutivo da espécie através da análise conjunta da histologia dos testículos e dos aspectos reprodutivos observados em campo, como distribuição temporal e espacial da espécie, abundância de machos em vocalização, casais em amplexo, desovas, e presença de formas larvais, juvenis e adultas. Vocalizações foram observadas ao longo de todo o período experimental, enquanto formas juvenis foram avistadas de setembro a abril. Nenhum casal em amplexo, nem desovas e girinos foram visualizados. Áreas recobertas por vegetação aquática e áreas marginais com gramíneas constituíram os principais sítios reprodutivos da espécie. Machos de D. minutus apresentaram variações anuais significativas no comprimento corporal, peso corporal, massa gonadal e no índice gonadossomático. Espermátides e espermatozóides foram observados nos túbulos seminíferos em todos os meses do ano, mostrando sincronia na atividade testicular. A partir da histologia dos testículos e dos aspectos reprodutivos observados em campo, podemos concluir que D. minutus possui ciclo reprodutivo prolongado, com maior potencial reprodutivo entre os meses de junho e dezembro.Item Bark extract of Bathysa cuspidata in the treatment of liver injury induced by carbon tetrachloride in rats(Brazilian Archives of Biology and Technology, 2014-07) Gonçalves, Reggiani Vilela; Matta, Sérgio Luis Pinto da; Novaes, Rômulo Dias; Leite, João Paulo Viana; Peluzio, Maria do Carmo Gouveia; Vilela, Emerson FerreiraThe aim of this study was to investigate the effect of bark extract Bathysa cuspidata (BCE) in the reconstitution of hepatic parenchyma and stroma after dysfunction induced by the CCl4. Liver lesions were induced by intraperitoneal administration of CCl4 (1 mL/kg) every 48 h for 12 days. The animals were treated with B. cuspidata extract (BCE) administered by gavage for another 12 days. Forty-nine rats were randomized into seven treatment groups with seven animals receiving CCl4, BCE (200, or 400 mg/kg) and the vehicle DMSO alone, or in different combinations. The extract alone showed no evidence of hepatic toxicity. In general, rats acutely exposed to CCl4 without the treatment with BCE presented high ALT and AST serum levels and high tissue content of lipid hydroperoxides, malondialdehyde, and lipid droplets. BCE administration, especially at 400 mg/kg attenuated significantly all these parameters. Furthermore, the antioxidant activities of the enzymes superoxide dismutase and catalase were significantly increased in the groups receiving this extract. These results showed that the extract of B. cuspidata stem bark stimulated the antioxidant defense system and reduced the morphological and functional liver damage in Wistar rats previously exposed to CCl4.Item Chia seed shows good protein quality, hypoglycemic effect and improves the lipid profile and liver and intestinal morphology of wistar rats(Plant Foods for Human Nutrition, 2016-05-19) Silva, Bárbara Pereira da; Dias, Desirrê Morais; Moreira, Maria Eliza de Castro; Toledo, Renata Celi Lopes; Matta, Sérgio Luis Pinto da; Lucia, Ceres Mattos Della; Martino, Hércia Stampini Duarte; Pinheiro-Sant’Ana, Helena MariaChia has been consumed by the world population due to its high fiber, lipids and proteins content. The objective was to evaluate the protein quality of chia untreated (seed and flour) and heat treated (90 °C/20 min), their influence on glucose and lipid homeostasis and integrity of liver and intestinal morphology of Wistar rats. 36 male rats, weanling, divided into six groups which received control diet (casein), free protein diet (aproteic) and four diet tests (chia seed; chia seed with heat treatment; chia flour and chia flour with heat treatment) for 14 days were used. The protein efficiency ratio (PER), net protein ratio (NPR) and true digestibility (TD) were evaluated. The biochemical variables and liver and intestinal morphologies of animals were determined. The values of PER, NPR and TD did not differ among the animals that were fed with chia and were lower than the control group. The animals that were fed with chia showed lower concentrations of glucose; triacylglycerides, low-density lipoprotein cholesterol and very low-density lipoprotein and higher high-density lipoprotein cholesterol than the control group. The liver weight of animals that were fed with chia was lower than the control group. Crypt depth and thickness of intestinal muscle layers were higher in groups that were fed with chia. The consumption of chia has shown good digestibility, hypoglycemic effect, improved lipid and glycemic profiles and reduced fat deposition in liver of animals, and also promoted changes in intestinal tissue that enhanced its functionality.Item Colon cancer and swimming exercise: effect on wistar rat testes(Brazilian Archives of Biology and Technology, 2013-05-09) Natali, Antônio José; Freitas, Karine Moura de; Lunz, Wellington; Costa, Kyvia Lugate Cardoso; Leite, Rodrigo Paula; Dolder, Heidi; Matta, Sérgio Luis Pinto daThis study was undertaken to determine whether colon cancer (CC) and chronic swimming exercise alter rat testis. Eleven weeks old rats were distributed into control group (n=6) and the groups that were induced to develop CC by dimethylhydrazine injections (nEG, EG0, EG2 and EG4; n=10 each group). In the group nEG, the rats did not swim, whereas groups EG0, EG2 and EG4, underwent a swimming program with distinct loads (0, 2 and 4% of body mass, respectively) for 35 weeks. The morphometry, stereology and cell counts showed damage caused by the CC on the germ epithelium. These results were noteworthy since this was the first report to associate the CC with testicular damage. Swimming exercise had no significant role in reducing, or increasing the CC effects on the testis, despite having slightly improved the testis structure of the exercised rats without load. In conclusion, CC caused testis impairment, which could not be avoided by the swimming exercise.Item Efeito da dieta hiperlipídica e do treinamento aeróbico na aterosclerose em camundongos apoE-/-(Revista Brasileira de Medicina do Esporte, 2013-10-04) Natali, Antônio José; Fernandes, Silvio Anderson Toledo; Matta, Sérgio Luis Pinto da; Teodoro, Bruno Gonzaga; Franco, Frederico Souzalima Caldoncelli; Laterza, Mateus Camaroti; Peluzio, Maria do Carmo GouveiaA população brasileira tem seguido a tendência dos países desenvolvidos, cuja característica é o aumento do consumo de gorduras e sedentarismo. Investigar os efeitos da dieta hiperlipídica e do treinamento sobre o peso corporal, o consumo alimentar, a massa corporal magra, os lipídios plasmáticos, o peso do fígado e a aterosclerose em camundongos com deficiência de apolipoproteína E (apoE-/-). vinte e seis camundongos foram divididos em grupos/dieta: NS (normolipídica e sedentário), HS (hiperlipídica e sedentário), NT (normolipídica e treinamento) e HT (hiperlipídica e treinamento). Aferiu-se peso corporal (PC), consumo alimentar (CA) e peso relativo do fígado (PRF). Calculou-se a água da carcaça (A%) pela diferença do peso pré e pós-secagem, gordura (G%) por Soxhlet e proteína (P%) por Kjeldahl. Determinaram-se colesterol total (CT), triglicerídeos (TG) e HDL por método enzimático-colorimétrico e LDL e VLDL pela formula de Friedewald. Analisou-se a aterosclerose por fotomicrografia. Utilizou-se ANOVA e o método de Duncan, com P < 0,05. No PC inicial e final, não houve diferença de G% e P% entre os grupos (P > 0,05). A dieta hiperlipídica aumentou o CA (365,3 ± 5,09 vs. 340,16 ± 6,32) e LDL (452,20 ± 114,63 vs. 329,60 ± 77,54) nos animais sedentários e o CT (858,75 ± 140,84 vs. 320,87 ± 157,81) e o LDL (839,36±139,94 vs. 282,66±166,92) nos treinados (P < 0,05). Já o treinamento aumentou o CA (350,4 ± 7,81 vs. 340,16 ± 6,32) e A% (64,23 ± 2,46 vs. 62,62 ± 1,47) nos camundongos normolipídicos e diminuiu o CA (336,68 ± 6,23 vs. 365,3 ± 5,09), TG (22,75 ± 12,36 vs. 66,00 ± 21,88), HDL (14,83 ± 3,95 vs. 37,60 ± 13,12), VLDL (4,55 ± 2,47 vs. 13,20 ± 4,37) e aterosclerose (0,35 ± 0,13 vs. 0,56 ± 0,12) nos hiperlipídicos (P < 0,05). A dieta hiperlipídica pode desencadear distúrbios no metabolismo lipídico e, com isso, desencadear doenças cardiovasculares; porém, associada ao treinamento, pode diminuir a aterosclerose.Item Efeito da exposição pré e pós-natal ao ultra-som de baixa intensidade sobre a célula de Leydig e demais componentes do espaço intertubular do testículo de camundongos adultos(Revista Ceres, 2004-07) Navarro, Rodrigo Diana; Paula, Tarcízio Antônio Rego de; Matta, Sérgio Luis Pinto da; Fonseca, Cláudio César; Neves, Marco Túlio David dasA estimulação ultrassônica de baixa intensidade (7,5 Mhz), fornecida por aparelhos comerciais de diagnóstico por imagem, quando aplicada no abdômen de camundongos nos últimos 10 dias de gestação e na região urogenital dos filhotes nos 15 dias seguintes ao parto, diariamente, por cinco minutos, levou a um aumento significativo dos pesos corporais e das glândulas vesiculares e da concentração sérica de testosterona nos animais adultos. Aumentou também a proporção volumétrica, o volume e o número de células de Leydig por testículo e o volume individual da célula de Leydig, embora somente o último parâmetro de forma estatisticamente significativa. A influência do ultrassom perinatal, em doses de diagnóstico clínico no testículo de camundongos, referiu-se principalmente a estímulo no crescimento individual da célula de Leydig antes de sua proliferação, o que refletiu em significativo aumento no nível sérico de testosterona. Influenciou ainda, positivamente, a proporção volumétrica de tecido conjuntivo intertubular e, negativa e significativamente, a proporção de vasos linfáticos.Item Effect of dry coffee residues fermented with Monascus ruber on the metabolism of Apo E mice(Arquivos Brasileiros de Cardiologia, 2012-03-23) Brito, Larissa Froede; Queirós, Lívia Dias de; Peluzio, Maria do Carmo Gouveia; Ribeiro, Sônia Machado Rocha; Matta, Sérgio Luis Pinto da; Queiroz, José Humberto deAtherosclerosis is a chronic inflammatory disease of multifactorial origin, which occurs in response to endothelial injury. The fungus Monascus ruber has hypocholesterolemic activity, and the polyphenols present in coffee residue have an antioxidant activity and can help prevent cardiovascular diseases. Coffee residue has a significant amount of fermentable sugars, being an adequate substrate for growing fungi. The objective of this study was to assess the effect of dry coffee residue fermented with Monascus ruber on the lipid metabolism of Apo E knockout mice. The biological assay was performed with 30 Apo E knockout mice, divided into five groups and undergoing different treatments. The phytochemical prospection and quantification of phenolic compounds of the fermented and non-fermented coffee residues were performed. The sera of the animals were analyzed by using enzyme kits, and the aortic tissue was embedded in paraffin and stained with hematoxylin and eosin to undergo histopathological analysis. Comparing with the control group, the group receiving 2% non-fermented coffee residue showed a reduction of 42% in the serum levels of triacylglycerols and of approximately 41% in VLDL-c. The groups receiving 10% non-fermented coffee residue and 2% fermented coffee residue showed reductions in the lesion areas of 10.5% and 15.4%, respectively, as compared with the control group. The fermented coffee residue showed a higher content of phenolic compounds as compared with the non-fermented coffee residue. The present study showed that coffee residue fermentation has a potentially beneficial effect on cardiovascular diseases, especially atherosclerosis.Item Histological alterations in liver and testis of Astyanax aff. bimaculatus caused by acute exposition to zinc(Revista Ceres, 2015-03-03) Santos, Daiane Cristina Marques dos; Cupertino, Marli do Carmo; Matta, Sérgio Luis Pinto da; Oliveira, Juraci Alves de; Santos, Jorge Abdala Dergam dosThis study investigated the effect of acute exposition to zinc (Zn) on histology of the liver and testes of yellow tail lambari (Astyanax aff. bimaculatus). The exposure consisted of six concentrations of Zn (0, 3, 5, 10, 15, and 20 mg/L) for 96 hours of exposure. Fragments of liver and testis were routinely processed and embedded in plastic resin based on glycol methacrylate. Fragments of bones, muscles, liver and testis were dehydrated and digested to quantify the absorption levels of Zn in the tissue. Acute exposure to concentrations above 10mg/L has produced structural changes in the liver and gonads. The changes found in the liver were vascular congestion; decrease of cellular volume; displacement of the hepatocyte nucleus; necrosis; disarrangement of cordon structure; leukocyte infiltrate and vacuolization. The changes found in the gonads were ruptured cyst, delayed development of germ cells, pyknotic nucleus, cell cluster, displacement of cyst wall and vacuolization. The histological changes observed were compatible with the increasing concentration of zinc in environment, compromising liver and reproductive functions, because there was an increase in relative frequency of hepatocytes and reduced sperm productionItem Improvements of Atherosclerosis and Hepatic Oxidative Stress are Independent of Exercise Intensity in LDLr-/- Mice(Journal of Atherosclerosis and Thrombosis, 2012-04-10) Teodoro, Bruno Gonzaga; Natali, Antônio José; Fernandes, Sílvio Anderson Toledo; Matta, Sérgio Luis Pinto da; Peluzio, Maria do Carmo Gouveia; Silva, Luciano Acordi da; Pinho, Ricardo Aurino deCardiovascular diseases are the main causes of death in the Western world and are manifested by atherosclerosis. Depending on its intensity, regular aerobic exercise may be either beneficial or harmful to the atherosclerosis process. The aim of this study was to verify the effects of aerobic exercise training of different intensities on the profile of atherosclerotic lesions and serum lipid, and in the hepatic oxidative balance of low-density lipoprotein receptor-deficient (LDLr-/-) mice previously developed with atherosclerosis. All animals were submitted to a three-month high-fat and high-cholesterol diet regime. The animals were then randomly divided into no exercise (G1, n=9), low-intensity aerobic exercise (G2, n=10, 8 weeks of treadmill running, 30 min/day−1 at 8-10 m/min−1) and moderate-intensity aerobic exercise (G3, n=10, 8 weeks of treadmill running, 30 min/day−1 at 10-16m/min−1) groups. Serum total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C) and triglycerides (TG), and oxidative damage (protein carbonyls and lipid hydroperoxides) were measured. The activity of catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GPx) in the liver tissue was assessed. G2 (0.015±0.005cm2) and G3 (0.014±0.001cm2) presented lower aortic fat deposition than G1 (0.039±0.005cm2). G2 and G3 exhibited higher HDL-C, TG and CAT activity, but lower lipid peroxidation and carbonyl protein than G1. SOD values were higher in G3 than G2 and G1, and GPx was higher in G2 than in G3 and G1. Our protocols of low- and moderate-intensity aerobic exercise training (30 min daily for 8 weeks) induced similar benefits in LDLr-/- mice with atherosclerosis.Item Investigation of liver tissue and biochemical parameters of adult wistar rats treated with Arctium lappa L.(Brazilian Archives of Biology and Technology, 2009-03) Predes, Fabrícia Souza; Matta, Sérgio Luis Pinto da; Monteiro, Juliana Castro; Oliveira, Tânia Toledo deThis study was carried out to evaluate the effects of Arctium lappa L. (burdock) on the liver of adult male Wistar rats as measured by light microscopy and biochemical parameters. The rats received the extract in water bottles at doses of 10 or 20 g/L daily for 40 days. There were no significant changes in the plasma levels of albumin, aspartate transaminase (AST), alanine transaminase (ALT), gamma glutamyl transferase (GGT), total protein, total cholesterol, urea, uric acid, triacylglycerol, calcium, phosphorus, chlorine and direct bilirubin. The morphological analysis did not reveal histopathological alterations in liver tissue. Both biochemical and morphological data did not indicate A. lappa toxicity.Item Kefir reduces the incidence of pre-neoplastic lesions in an animal model for colorectal cancer(Journal of Functional Foods, 2019-02) Reis, Sandra Aparecida dos; Conceição, Lisiane Lopes da; Dias, Mariana Moura e; Siqueira, Nathane Paes; Rosa, Damiana Diniz; Oliveira, Leandro Licursi de; Matta, Sérgio Luis Pinto da; Peluzio, Maria do Carmo GouveiaThis study aimed to evaluate the effect of regular consumption of milk kefir on the development of pre-neoplastic colonic lesions. Thirty Wistar rats received water (Control group) or milk (Milk group) or kefir (Kefir group) during five weeks. After that, colonic lesions were chemically induced and the treatments continued for more thirteen weeks. The regular consumption of kefir was able to reduce the incidence of aberrant crypt foci by 36%. Also, the consumption of kefir increased the cecal concentration of short chain fatty acids; reduced the lactulose/mannitol ratio; promoted an increase in the colonic concentration of TNF-α and IL-1β, and the enzyme catalase in comparison with the control group. Thus, kefir reduced the development of lesions, probably by increasing the production of short chain fatty acids; reduction of intestinal permeability; immunomodulation and improvement of colonic antioxidant activitItem Microscopic morphology and testis morphometry of captivity- bred Adult bullfrogs ( Lithobates catesbeianus Shaw, 1802)(Brazilian Archives of Biology and Technology, 2009-11) Carlos, Jaqueline; Matta, Sérgio Luis Pinto daThe aim of this work was to study the testicular morphometry of captivity-bred adult bullfrogs. Fifteen young adult male were studied, in the rainy season and a lengthy photoperiod. The GSI was established at 0.15%. The nuclear diameter of germinative and Leydig cells, the nucleolus diameter of Sertoli cells and the area of cysts and tubules were determined and the mean number of ISPC, IISPC and SPT per cyst and the mean number of cysts per tubule was estimated. The nucleoplasmatic proportion of the nucleus of the Leydig cell was 76.22%, indicating less cytoplasmic activity. Eight generations of spermatogonia were found. The spermatogenesis efficiency in meiosis and in mitosis was 63 and 49%, respectively. The spermatogenesis of bullfrog fited in the pattern of other captivity Anurans, with differences as the morphology of Sertoli and Leydig cells nuclei.Item Monitoramento por radiotelemetria da área de uso de onça parda reintroduzida no entorno do Parque Estadual da Serra do Brigadeiro – MG, Brasil(Ciência Rural, 2011-07) Barros, João Bosco Gonçalves de; Paula, Tarcizio Antônio Rego de; Melo, Fabiano Rodrigues de; Matta, Sérgio Luis Pinto da; Souza, Thyara Deco; Araujo, Gediendson Ribeiro; Csermak Júnior, Antônio Carlos; Ávila, Eduardo Costa; Garay, Rafael MoraisUm exemplar macho, subadulto de onça parda reintroduzido foi monitorado por técnica de radiotelemetria. O monitoramento foi de 110 dias, realizado de forma aleatória, com intervalos descontínuos. Os dados do acompanhamento sugerem que a área utilizada por este espécime seja de, aproximadamente, 26km2. Esta configura o menor índice já descrito para a espécie, porém, deve-se ressaltar a importância deste monitoramento básico, visto a incipiência do comportamento de um felino de grande porte reintroduzido em habitat natural após período em cativeiro.Item Morfometria testicular e dos túbulos seminíferos de leões-africanos (Panthera leo, Linnaeus, 1758) adultos, em cativeiro(Revista Ceres, 2006-11) Barros, João Bosco Gonçalves; Paula, Tarcizio Antonio Rego de; Matta, Sérgio Luis Pinto da; Fonseca, Cláudio César; Melo, F.R.; Benjamim, Laércio dos AanjosUma relação direta entre peso testicular e produção espermática é observada em diferentes animais já investigados. O tamanho testicular reflete principalmente o comprimento total do túbulo seminífero, visto ser este seu principal componente. A composição do parênquima testicular e o tamanho relativo dos testículos podem fornecer valiosas informações à fisiologia da reprodução. O objetivo deste trabalho foi quantificar os diferentes componentes testiculares em leões africanos adultos mantidos em cativeiro. Este estudo concluiu que o peso corporal médio dos leões-africanos neste estudo foi de 183,7 kg, dos quais cerca de 0,015% é de massa testicular e 0,009% de túbulos seminíferos, que representaram cerca de 75,6% do parênquima testicular. O diâmetro médio dos túbulos seminíferos foi de 252,7mm e a espessura média do epitélio seminífero, de 93,2mm. O leão-africano apresenta 12,3 metros de túbulo seminífero por grama de testículo.Item Morphometry of testis and seminiferous tubules of the adult crab-eating fox (Cerdocyon thous, Linnaeus, 1766)(Revista Ceres, 2010-08) Caldeira, Bianca Cabral; Paula, Tarcízio Antônio Rêgo de; Matta, Sérgio Luis Pinto da; Balarini, Mayte Koch; Campos, Pamella Kelly AraújoBody and testicular biometric parameters are very important for establishing reproductive patterns and, consequently, the development of protocols for assisted reproduction in different species. A direct correlation between the testis weight and the sperm population was observed in other studied species, because the testis size primarily reflects the total volume of the seminiferous tubule, its main component. The objective of this study was to determine the testicular volume parameters and correlate data from morphometry of testis and seminiferous tubules with body mass in six adult crab-eating foxes. The mean body weight of the crab-eating foxes in this study was 6.53 kg, with approximately 0.068% allocated to the testicular mass and 0.042% specifically to seminiferous tubules, which represented 87.5% of the testicular parenchyma. The albuginea comprised 12.5% of the testicular mass. The mean diameter of seminiferous tubules was 236 µm, and the mean thickness of the seminiferous epithelium was 62.9 µm. Values of tubular parameters indicate a sperm productivity close to those observed in previously studied carnivores.Item Níveis de energia digestível na dieta de piauçu (Leporinus macrocephalus) no desenvolvimento testicular em estágio pós-larval(Zootecnia Tropical, 2006-01) Navarro, Rodrigo Diana; Matta, Sérgio Luis Pinto da; Lanna, Eduardo Arruda Teixeira; Donzele, Juarez Lopes; Rodrigues, Sirlene Souza; Silva, Rodrigo Fortes da; Calado, Leonardo Luiz; Ribeiro Filho, Oswaldo PintoObjetivando-se determinar o efeito do nível de energia digestível da dieta no desenvolvimento testicular de piauçu (Leporinus macrocephalus) foram utilizados 600 peixes com peso e comprimento médio inicial de 0,33 ± 0,11 g e 2,94 ± 0,39 cm, respectivamente. Foram realizados quatro tratamentos (2.600, 2.700, 2.800 e 2.900 kcal de ED/kg de ração). Verificou-se que o nível de energia não influenciou o peso corporal médio final e comprimento total. Foi verificado efeito quadrático (P<0,02) no peso da gônada aos 60 dias. Foi verificado efeito quadrático (P<0,02) no índice gonadossomático aos 60 dias. Os peixes do tratamentos com 2.800 e 2.900 kcal de ED/kg apresentaram nítida formação de cistos de espermatogônias primárias e secundárias. Os animais dos grupos com 2.600 e 2.700 kcal de ED/kg apresentaram formação de cistos de espermatogônias primária, apenas.Item Parâmetros biométricos corporais e testiculares de Jaguatiricas (Leopardus pardalis) adultas(Revista Ceres, 2009-03) Sarti, Priscilla; Paula, Tarcízio Antônio Rego de; Matta, Sérgio Luis Pinto da; Fonseca, Cláudio César; Polli, Gabriela de Oliveira; Balarini, Maytê Koch; Mascarenhas, Rebeca MarquesOs parâmetros da biometria corporal e morfofisiologia testicular são de suma importância no conhecimento da biologia reprodutiva, principalmente em correlação com os aspectos comportamentais dos sistemas de acasalamento, e, mesmo, no desenvolvimento de protocolos para reprodução assistida em espécies da fauna silvestre, essencialmente aquelas ameaçadas de extinção. Os carnívoros, notadamente os felinos, por ocuparem o topo da cadeia alimentar, são vítimas constantes de praticamente todas as formas de ameaça, sendo ainda, extremamente vulneráveis às altera- ções do “habitat”. No presente trabalho foram estudadas cinco jaguatiricas, machos adultos, proveniente de cativeiro, visando à coleta de informações biométricas corporais e testiculares. Os animais tinham, em média, 5,6 anos e pesaram 14,52 + 1,78 quilos. O tamanho corporal médio foi 89,2 + 5,1 cm e o diâmetro torácico 48,50 + 1,5 cm. O volume testicular direito médio foi 8,5 + 1,3 mL e esquerdo 8,6 + 0,9 mL. Uma vez que a densidade volumétrica do tecido testicular mamífero é de aproximadamente 1,0, o peso de ambos os testículos correspondeu em média a 17,13 + 2,16 g, equivalendo a uma alocação de 0,12 + 0,03 % do peso corporal em gônadas, ultrapassando os valores encontrados os felinos de maior porte, como leões africanos (0,015%), onças pintadas (0,034%) e onças pardas (0,03%) e inesperadamente maior do que aquele computado para felinos de menor porte. Os dados biométricos obtidos conduzem com registros de outros felinos adultos, podendo ser empregados para predizer o “status” de maturidade sexual. O diâmetro torácico de jaguatiricas machos adultas, por apresentar alta correlação com o peso corporal, é um parâmetro confiável na previsão deste.Item Perfil biométrico, histomorfométrico e bioquímico no tratamento com atorvastatina cálcica de ratas com osteoporose induzida com dexametasona(Revista Brasileira de Ortopedia (English Edition), 2018-09) Ferreira Junior, Davilson Bragine; Pizziolo, Virgínia Ramos; Oliveira, Tânia Toledo de; Matta, Sérgio Luis Pinto da; Píccolo, Mayra Soares; Queiroz, José Humberto deAvaliar os efeitos da atorvastatina cálcica no tratamento da osteoporose induzida com dexametasona. A indução da osteoporose consistiu na administração de dexametasona na dose de 7,5 mg/kg de peso corporal, por via intramuscular, uma vez por semana durante quatro semanas, à exceção dos animais do grupo controle (G1). Os animais foram distribuídos nos seguintes grupos: G1 (grupo controle sem osteoporose), G2 (grupo controle com osteoporose sem tratamento), G3 (grupo controle com osteoporose tratado com alendronato de sódio 0,2 mg/kg) e G4 (grupo com osteoporose tratado com atorvastatina cálcica 1,2 mg/kg). Após 30 e 60 dias do início do tratamento dos animais, foram feitas as dosagens dos níveis séricos de fosfatase alcalina, fosfatase alcalina óssea, avaliação biométrica e histomorfométrica óssea. Em relação às análises biométricas e histomorfométricas, aos 60 dias de tratamento o G4 apresentou densidade óssea (índice Seedor), densidade trabecular óssea e espessura da cortical de 0,222 ± 0,004 g/cm, 59,167 ± 2,401% e 387,501 ± 8,573 μm, respectivamente, com diferença positiva, estatisticamente significativa (p < 0,05), em relação ao grupo G2. Aos 30 e 60 dias de tratamento, o G4 apresentou níveis séricos de fosfatase alcalina óssea estatisticamente significativos (p < 0,05) e superiores a todos os grupos (7,451 ± 0,173 μg/L e 7,473 ± 0,529 μg/L, respectivamente). O tratamento com atorvastatina cálcica demonstrou a capacidade desse fármaco de aumentar a atividade osteoblástica e a atividade reparadora tecidual óssea, atuar de forma diferente do alendronato de sódio, que demonstrou atividade preponderantemente antirreabsortiva.Item Reproductive biology of Oligosarcus argenteus (Gunther, 1864) adult males and description of the gonadal maturation stages(Brazilian Archives of Biology and Technology, 2009-01) Gomes, Marcos de Lucca Moreira; Andrade, Dálcio Ricardo de; Matta, Sérgio Luis Pinto daOligosarcus argenteus belongs to the Acestrorhynchinae subfamily, being restricted to South America, and found in several Brazilian hydrographic basins, in lotic and lentic environments, where they are able to reproduce. With the purpose of studying the reproductive biology of the males from this species, many morphological parameters were analyzed during a 24 month period, as well as characterizing the different testicular maturation stages. A maturity scale, with three stages (I – Initial Maturing, II – Intermediate Maturing, III – Final Maturing) was proposed for the adult males of Oligosarcus argenteus. The reproductive period was established by the bimonthly frequency of spermatogenesis and by the gonadal maturation stages.Item The seminiferous epithelium cycle and daily spermatic production in the adult maned wolf (Chrysocyon brachyurus, Illiger, 1811)(Micron, 2006-10-21) Bitencourt, Viviane Lewicki; Paula, Tarcı́zio Antônio Rego de; Matta, Sérgio Luis Pinto da; Fonseca, Cláudio César; Benjamin, Laércio dos Anjos; Costa, Deiler SampaioThe duration of the seminiferous epithelium cycle was estimated in adult maned wolves (Chrysocyon brachyurus, Illiger, 1811), by applying intratesticular injections with tritiated thymidine. The total duration of the seminiferous epithelium cycle in this species was calculated in 8.99 days. So, taking into account that approximately 4.5 cycles of the seminiferous epithelium are necessary for the whole spermatogenesis process to complete, the production of spermatozoa from one spermatogonia will take about 40.45 days. The duration of the spermiogenesis was calculated to be 12.3 days. The eight stages of the seminiferous epithelium cycle were described by the tubular morphology method, which is based either on the form and position of the spermatid nuclei and the occurrence of meiotic divisions. The values of the relative frequency for the pre-meiotic, meiotic and post-meiotic phases in this species were 3.5, 0.78 and 4.8 days, respectively. The maned wolf produces about 29 million spermatozoa a day for each testis gram, therefore being classified among the species provided with a high spermatogenetic efficiency.