Navegando por Autor "Haddi, Khalid"
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Item Detection of a new pyrethroid resistance mutation (V410L) in the sodium channel of Aedes aegypti: a potential challenge for mosquito control(Scientific Reports, 2017-04-19) Martins, Gustavo F.; Haddi, Khalid; Tomé, Hudson V. V.; Du, Yuzhe; Valbon, Wilson R.; Nomura, Yoshiko; Dong, Ke; Oliveira, Eugênio E.The yellow fever mosquito, Aedes aegypti, particularly in Neotropical regions, is the principal vector of dengue, yellow fever, Zika and Chikungunya viruses. Pyrethroids remain one of the most used insecticides to control Aedes mosquitoes, despite the development of pyrethroid resistance in many mosquito populations worldwide. Here, we report a Brazilian strain of A. aegypti with high levels (approximately 100–60,000 fold) of resistance to both type I and type II pyrethroids. We detected two mutations (V410L and F1534C) in the sodium channel from this resistant strain. This study is the first report of the V410L mutation in mosquitoes. Alone or in combination with the F1534C mutation, the V410L mutation drastically reduced the sensitivity of mosquito sodium channels expressed in Xenopus oocytes to both type I and type II pyrethroids. The V410L mutation presents a serious challenge for the control of A. aegypti and will compromise the use of pyrethroids for the control of A. aegypti in Brazil; therefore, early monitoring of the frequency of the V410L mutation will be a key resistance management strategy to preserve the effectiveness of pyrethroid insecticides.Item Diversity and convergence of mechanisms involved in pyrethroid resistance in the stored grain weevils, Sitophilus spp.(Scientific Reports, 2018) Haddi, Khalid; Valbon, Wilson R.; Jumbo, Luis O. Viteri; Oliveira, Luiz O. de; Guedes, Raul N. C.; Oliveira, Eugenio E.Target-site mutations and changes in insect metabolism or behavior are common mechanisms in insecticide-resistant insects. The co-occurrence of such mechanisms in a pest strain is a prominent threat to their management, particularly when alternative compounds are scarce. Pyrethroid resistance among stored grain weevils (i.e., Sitophilus spp.) is an example of a long-standing concern, for which reports of resistance generally focus on a single mechanism in a single species. Here, we investigated pyrethroid resistance in maize and rice weevils (i.e., Sitophilus zeamais and S. oryzae), exploring potential knockdown resistance (kdr) mutations in their sodium channels (primary site for pyrethroid actions) and potential changes in their detoxification and walking processes. Resistance in pyrethroid- resistant rice weevils was associated with the combination of a kdr mutation (L1014F) and increases in walking and detoxification activities, while another kdr mutation (T929I) combined with increases in walking activity were the primary pyrethroid resistance mechanisms in maize weevils. Our results suggest that the selection of pyrethroid-resistant individuals in these weevil species may result from multiple and differential mechanisms because the L1014F mutation was only detected in Latin American rice weevils (e.g., Brazil, Argentina and Uruguay), not in Australian and Turkish rice weevils or Brazilian maize weevils.Item Essential oil of Siparuna guianensis as an alternative tool for improved lepidopteran control and resistance management practices(Scientific Reports, 2018-05-08) Lourenço, Adriano M.; Haddi, Khalid; Ribeiro, Bergman M.; Corrêia, Roberto F. T.; Tomé, Hudson V. V.; Santos-Amaya, Oscar; Pereira, Eliseu J. G.; Guedes, Raul N. C.; Santos, Gil R.; Oliveira, Eugênio E.; Aguiar, Raimundo W. S.Although the cultivation of transgenic plants expressing toxins of Bacillus thuringiensis (Bt) represents a successful pest management strategy, the rapid evolution of resistance to Bt plants in several lepidopteran pests has threatened the sustainability of this practice. By exhibiting a favorable safety profile and allowing integration with pest management initiatives, plant essential oils have become relevant pest control alternatives. Here, we assessed the potential of essential oils extracted from a Neotropical plant, Siparuna guianensis Aublet, for improving the control and resistance management of key lepidopteran pests (i.e., Spodoptera frugiperda and Anticarsia gemmatalis). The essential oil exhibited high toxicity against both lepidopteran pest species (including an S. frugiperda strain resistant to Cry1A.105 and Cry2Ab Bt toxins). This high insecticidal activity was associated with necrotic and apoptotic effects revealed by in vitro assays with lepidopteran (but not human) cell lines. Furthermore, deficits in reproduction (e.g., egg-laying deterrence and decreased egg viability), larval development (e.g., feeding inhibition) and locomotion (e.g., individual and grouped larvae walking activities) were recorded for lepidopterans sublethally exposed to the essential oil. Thus, by similarly and efficiently controlling lepidopteran strains susceptible and resistant to Bt toxins, the S. guianensis essential oil represents a promising management tool against key lepidopteran pests.Item Locomotory and physiological responses induced by clove and cinnamon essential oils in the maize weevil Sitophilus zeamais(Pesticide Biochemistry and Physiology, 2015-06-14) Correa, Yenis Del Carmen Gonzales; Faroni, Lêda R.A.; Haddi, Khalid; Oliveira, Eugênio E.; Pereira, Eliseu José G.Plant essential oils have been suggested as a suitable alternative for controlling stored pests worldwide. However, very little is known about the physiological or behavioral responses induced by these compounds in insect populations that are resistant to traditional insecticides. Thus, this investigation evaluated the toxicity (including the impacts on population growth) as well as the locomotory and respiratory responses induced by clove, Syzygium aromaticum L., and cinnamon, Cinnamomum zeylanicum L., essential oils in Brazilian populations of the maize weevil Sitophilus zeamais. We used populations that are resistant to phosphine and pyrethroids (PyPhR), only resistant to pyrethroids (PyR1 and PyR2) or susceptible to both insecticide types (SUS). The PyPhR population was more tolerant to cinnamon essential oil, and its population growth rate was less affected by both oil types. Insects from this population reduced their respiratory rates (i.e., CO2 production) after being exposed to both oil types and avoided (in free choice-experiments) or reduced their mobility on essential oil-treated surfaces. The PyR1 and PyR2 populations reduced their respiratory rates, avoided (without changing their locomotory behavior in no-choice experiments) essential oil-treated surfaces and their population growth rates were severely affected by both oil types. Individuals from SUS population increased their mobility on surfaces that were treated with both oil types and showed the highest levels of susceptibility to these oils. Our findings indicate that S. zeamais populations that are resistant to traditional insecticides might have distinct but possibly overlapping mechanisms to mitigate the actions of essential oils and traditional insecticides.Item Mosquiticidal and repellent potential of formulations containing wood residue extracts of a Neotropical plant, Tabebuia heptaphylla(Industrial Crops and Products, 2019-03) Haddi, Khalid; Oliveira, Eugenio E.; Borges, Jaqueline C. M.; Andrade, Bruno Silva; Nascimento, Vitor L.; Melo, Tarcísio Silva; Didonet, Julcemar; Carvalho, Jose C. T.; Cangussu, Alex S.; Soares, Ilsamar M.; Ascencio, Sergio D.; Raposo, Nádia R. B.; Aguiar, Raimundo W. S.The use of wood residues and their extracts as sources for bioactive molecules not only helps to reduce the environmental impacts of the wastage produced by timber industry but also provide innumerous biotechnological application opportunities, including the development of novel insecticide products. Here, the insecticidal and repellent (in gel and cream formulations) potential of wood extracts of the Neotropical plant “purple ipê”, Tabebuia heptaphylla (Bignoniaceae) against the mosquitoes Aedes aegypti are investigated. Firstly, the chemical composition of the chloroform and hexane extracts obtained from T. heptaphylla residues is analyzed and their insecticidal activities are assessed against A. aegypti larvae. Furthermore, the repellence and oviposition deterrence actions of these extracts were evaluated against adult mosquito females. Computational docking analysis was also conducted to predict the physical interactions between the major constituents of the T. heptaphylla residue extracts and the odorant binding receptor of A. aegypti. Despite the fact that the 2,6-di-tert-butylnaphthalene was the major constituent (53.3%) in the T. heptaphylla hexanoic extract, the presence of lapachol, 2-hydroxy-3-(3-methyl-2-butenyl)-1,4-naphthalenedione, as major constituent in both wood extracts combined with the computational docking analysis, strongly suggest the lapachol as the major factor of the insecticidal/repellent effects of T. heptaphylla wood extracts. The computational docking analysis predicted significant binding of lapachol with the internal active pocket of the mosquito odorant binding receptor, which helps to explain the up to 3 h protection against bites of A. aegypti females provided by gel and cream formulations containing the T. heptaphylla residue extracts. Collectively, the findings demonstrated relevant biotechnological and environmentally-friend opportunities (i.e., production of biopesticides) for the wood residues produced by timber activities during the extraction and processing of T. heptaphylla trees.Item Pyrethroid resistance is associated with a kdr‐type mutation (L1014F) in the potato tuber moth Tecia solanivora(Pest Management Science, 2016-08-08) Bacca, Tito; Haddi, Khalid; Pineda, Maria; Guedes, Raul Narciso C; Oliveira, Eugênio E.The Guatemalan potato tuber moth, Tecia solanivora, has been the most important pest species in Hispanico‐American potato fields since its first record on potatoes in 1956 in Guatemala. This insect pest has been spreading to other parts of the world, including the Canary Islands in Europe. Tuber moth control relies heavily on the use of insecticides, including pyrethroids. Here, we assessed the likelihood of control failures and performed concentration–response bioassays in five Colombian strains of T. solanivora to evaluate their susceptibilities to the pyrethroid permethrin. Evidence of control failures was observed in four strains tested, which exhibited moderate resistance levels (i.e. ranging from 5.4‐ to 24.4‐fold). However, no spatial dependence was observed between the permethrin LC50 values and the geographic distances among the tuber moth strains. In order to evaluate whether permethrin resistance was mediated by potential mutations in the para‐type sodium channels of T. solanivora, the IIS4–IIS6 region of the para gene was PCR amplified and sequenced from the five strains tested. As demonstrated across a range of different arthropod species that exhibited knockdown resistance (kdr), we observed a single point substitution (L1014F) at high frequencies in the para gene of all four resistant strains. This is the first identification of a target‐site‐alteration‐based resistance in the Guatemalan potato tuber moth T. solanivora, which is widespread and exhibits high frequencies among geographically distant strains, indicating that pyrethroids are probably becoming ineffective for the control of this pest species.Item Repellency and bioactivity of Caatinga biome plant powders against Callosobruchus maculatus (Coleoptera: Chrysomelidae: Bruchinae)(Florida Entomologist, 2015) Melo, Bruno Adelino de; Molina- Rugama, Adrián José; Haddi, Khalid; Leite, Delzuite Teles; Oliveira, Eugênio Eduardo deThe Caatinga biome represents the 4th-largest area covered by single vegetation in Brazil and contains dry forests rich in aromatic bushes, vines, herbs, and trees. The flora of this ecological region is widely known and employed in folk medicine and has other utilitarian and econom- ic uses; however, its potential for controlling or repelling insects is poorly investigated. In this study, we evaluated the potential use of Caatinga plant species for controlling infestations of Callosobruchus maculatus F. (Coleoptera: Chrysomelidae: Bruchinae), the most important insect pest of cowpea, Vigna unguiculata (L.) Walp. (Fabales: Fabaceae). Powders of the leaves and stems of 9 plant species, including Amburana cearensis A. C. Smith (“cumaru”) (Fabales: Fabaceae), Croton sonderianus Müll. Arg. (“marmeleiro”) (Malpighiales: Euphorbiaceae), Cleome spinosa Jacq. (“mussambê”) (Capparales: Cleomaceae), Mimosa tenuiflora Benth. (“jurema-preta”) (Fabales: Fabaceae), Anadenanthera mac- rocarpa (Benth.) Brenan (“angico-vermelho”) (Fabales: Fabaceae), Aspidosperma pyrifolium Mart. (“pereiro”) (Gentianales: Apocynaceae), Senna occidentalis (L.) H.S. Irwin & R.C. Barneby (“manjerioba”) (Fabales: Fabaceae), Hyptis suaveolens (L.) Poit. (“alfazema-brava”) (Lamiales: Lamiaceae), and Ziziphus joazeiro Mart. (“juazeiro”) (Rosales: Rhamnaceae), were applied on masses of cowpea seeds, and their effects on C. maculatus longevity as well as their repellent activities were evaluated. All the leaf and stem powders reduced only the longevity of males and showed strongly repellent activities against females. The preference level of females for untreated beans varied between 73 and 94%, indicat- ing that all the leaf and stem powders can be a part of the integrated management of C. maculatus in storage facilities.Item Sexual success after stress? Imidacloprid Induced Hormesis in males of the Neotropical Stink Bug Euschistus heros(Plos One, 2016-06-10) Barcellos, Marcelo S.; Haddi, Khalid; Mendes, Marcos V.; Lino-Neto, José; Freitas, Hemerson L.; Guedes, Raul Narciso C.; Oliveira, Eugênio E.Environmental stress in newly-emerged adult insects can have dramatic consequences on their life traits (e.g., dispersion, survival and reproduction) as adults. For instance, insects sublethally exposed to environmental stressors (e.g., insecticides) can gain fitness benefits as a result of hormesis (i.e., benefits of low doses of compounds that would be toxic at higher doses). Here, we experimentally tested whether sublethal exposure to the insecticide imidacloprid would hormetically affect the sexual fitness of newly-emerged adults of the Neotropical brown stink bug Euschistus heros (Hemiptera: Heteroptera: Pentatomidae), which is the most abundant and prevalent insect pest in Neotropical soybean fields. We evaluated the sexual fitness of four couple combinations: unexposed couples, exposed females, exposed males, and exposed couples. Sublethal exposure to dry residues (i.e., contact) of imidacloprid (at 1% of recommended field rate) did not affect insect survival, but led to higher mating frequencies when at least one member of the couple was exposed. However, the average mating duration was shortened when only females were exposed to imidacloprid. Moreover, exposed males showed higher locomotory (walking) activity, lower respiration rates and induced higher fecundity rates when mated to unexposed females. Although the reproductive tracts of exposed males did not differ morphometrically from unexposed males, their accessory glands exhibited positive reactions for acidic and basic contents. Our findings suggest that males of the Neotropical brown stink bug hormetically increase their sexual fitness when cued by impending insecticidal stress in early adulthood.Item Toxicity to and egg‐laying avoidance of Drosophila suzukii (Diptera: Drosophilidae) caused by an old alternative inorganic insecticide preparation(Pest Management Science, 2017-10-24) Andreazza, Felipe; Ajila, Henry E Vacacela; Haddi, Khalid; Colares, Felipe; Pallini, Angelo; Oliveira, Eugênio EThe application of synthetic insecticides remains the most used tool for the management of spotted‐wing drosophila, Drosophila suzukii (Matsumura) (Diptera: Drosophilidae). However, management of this pest in the organic production of soft‐skinned fruits is a complex task due to the restricted number of registered products. Here, we assess the toxicity of lime sulfur and evaluate whether lime sulfur‐treated strawberry plants affected the oviposition and development of D. suzukii. Lime sulfur exhibited adequate toxicity to D. suzukii (LC50 = 26.6 mL L−1) without phytotoxicity to strawberry plants. When D. suzukii females were exposed to lime sulfur‐treated plants in no‐choice bioassays, oviposition was significantly (t‐test, P < 0.05) reduced compared with that on untreated plants. In free‐choice bioassays, D. suzukii females laid significantly (paired t‐test, P < 0.05) more eggs on untreated plants. Furthermore, in the free‐choice bioassays, immature development was slower for adults that originated from eggs laid on lime sulfur‐treated plants than from those laid on untreated plants. Lime sulfur showed adequate control and, therefore, has potential for use as a management tool against D. suzukii infestations in organic production systems. This old, alternative insecticide preparation not only caused adult fly mortality, but also reduced the number of eggs laid on lime sulfur‐treated plants.Item Toxicity to, oviposition and population growth impairments of Callosobruchus maculatus exposed to clove and cinnamon essential oils(Plos One, 2018) Jumbo, Luis Oswaldo Viteri; Haddi, Khalid; Faroni, Lêda Rita D.; Heleno, Fernanda F.; Pinto, Frederico G.; Oliveira, Eugênio E.The use of plant essential oils has been shown to efficiently control insect pests of stored beans, significantly reducing the threats associated with synthetic insecticides. Here, we evaluated the potential of applications of essential oils of clove, Syzygium aromaticum L., and cinnamon, Cinnamomum zeylanicum L., to control Callosobruchus maculatus, consid- ered as one of the most cosmopolitan pests of stored beans. Using four combinations of couples (i.e., unexposed couples, exposed females, exposed males, and exposed couples), we also evaluated how sublethal exposure to these essential oils impacted C. maculatus oviposition. Bioassays results revealed that both essential oils exhibited insecticidal activities similar to the synthetic pyrethroid insecticide deltamethrin. Furthermore, oil dosage increments proportionately decreased the growth rate and reduced the losses in bean weight caused by cowpea weevils, and offspring emergence was almost abolished when parents were exposed to the LD 20 of each essential oil. Finally, significant oviposition impairments were perceived only in couples where females were exposed (i.e., females exposed and exposed couples) to the LD 20 of cinnamon and clove essential oils. Thus, by exhibiting similar insecticidal activities as synthetic insecticides and by significantly affecting the ovipo- sition of sublethally exposed C. maculatus females, the cinnamon and clove essential oils represent valuable tools with potential of integration into the management of C. maculatus infestations.