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https://locus.ufv.br//handle/123456789/19436
Tipo: | Artigo |
Título: | Characterization of an exoinulinase produced by Aspergillus terreus CCT 4083 grown on sugar cane bagasse |
Autor(es): | Coitinho, Juliana B. Guimarães, Valéria M. Almeida, Maíra N. de Falkoski, Daniel L. Queiróz, José H. de Rezende, Sebastião T. de |
Abstract: | Exoinulinase (β- D -fructan fructohydrolase, EC 3.2.1.80) secreted by Aspergillus terreus CCT4083 was obtained using sugar cane bagasse, an agroindustrial residue, as a carbon source. It was further purified from the supernatant culture in a rapid procedure. The enzyme presented 57 kDa on SDS-PAGE and 56 kDa on gel filtration chromatography. Inulin was hydrolyzed by the purified enzyme, yielding D -fructose as the main product. This enzyme showed maximum activity at pH 4.0 and 60 °C and maintained more than 90 and 75% of its original activity at 40 and 50 °C, respectively, after 3.5 h of preincubation. The K M values for inulin, sucrose, and raffinose were 11, 4.20, and 27.89 mM, respectively, and D -fructose was a competitive inhibitor (K i = 47.55 mM). The activation energies for sucrose, raffinose, and inulin were 10.4, 5.61, and 4.44 kcal/mol, respectively. The characteristics of A. terreus exoinulinase were compared to those of inulinases isolated from other organisms. The exoinulinase traits presented especially good thermostability and the ability to produce pure D -fructose, suggesting its application to the production of high-fructose syrup. |
Palavras-chave: | Inulinase Aspergillus terreus CCT4083 inulin Agroindustrial residue |
Editor: | Journal of Agricultural and Food Chemistry |
Tipo de Acesso: | American Chemical Society |
URI: | https://doi.org/10.1021/jf1011159 http://www.locus.ufv.br/handle/123456789/19436 |
Data do documento: | 7-Fev-2010 |
Aparece nas coleções: | Artigos |
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Arquivo | Descrição | Tamanho | Formato | |
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artigo.pdf Until 2100-12-31 | Texto completo | 2,69 MB | Adobe PDF | Visualizar/Abrir ACESSO RESTRITO |
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