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Campo DC | Valor | Idioma |
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dc.contributor.author | Coitinho, Juliana B. | |
dc.contributor.author | Guimarães, Valéria M. | |
dc.contributor.author | Almeida, Maíra N. de | |
dc.contributor.author | Falkoski, Daniel L. | |
dc.contributor.author | Queiróz, José H. de | |
dc.contributor.author | Rezende, Sebastião T. de | |
dc.date.accessioned | 2018-05-10T12:08:58Z | |
dc.date.available | 2018-05-10T12:08:58Z | |
dc.date.issued | 2010-02-07 | |
dc.identifier.issn | 15205118 | |
dc.identifier.uri | https://doi.org/10.1021/jf1011159 | |
dc.identifier.uri | http://www.locus.ufv.br/handle/123456789/19436 | |
dc.description.abstract | Exoinulinase (β- D -fructan fructohydrolase, EC 3.2.1.80) secreted by Aspergillus terreus CCT4083 was obtained using sugar cane bagasse, an agroindustrial residue, as a carbon source. It was further purified from the supernatant culture in a rapid procedure. The enzyme presented 57 kDa on SDS-PAGE and 56 kDa on gel filtration chromatography. Inulin was hydrolyzed by the purified enzyme, yielding D -fructose as the main product. This enzyme showed maximum activity at pH 4.0 and 60 °C and maintained more than 90 and 75% of its original activity at 40 and 50 °C, respectively, after 3.5 h of preincubation. The K M values for inulin, sucrose, and raffinose were 11, 4.20, and 27.89 mM, respectively, and D -fructose was a competitive inhibitor (K i = 47.55 mM). The activation energies for sucrose, raffinose, and inulin were 10.4, 5.61, and 4.44 kcal/mol, respectively. The characteristics of A. terreus exoinulinase were compared to those of inulinases isolated from other organisms. The exoinulinase traits presented especially good thermostability and the ability to produce pure D -fructose, suggesting its application to the production of high-fructose syrup. | en |
dc.format | pt-BR | |
dc.language.iso | eng | pt-BR |
dc.publisher | Journal of Agricultural and Food Chemistry | pt-BR |
dc.relation.ispartofseries | v. 58, n. 14, p. 8386–8391, July 2010 | pt-BR |
dc.rights | American Chemical Society | pt-BR |
dc.subject | Inulinase | pt-BR |
dc.subject | Aspergillus terreus CCT4083 | pt-BR |
dc.subject | inulin | pt-BR |
dc.subject | Agroindustrial residue | pt-BR |
dc.title | Characterization of an exoinulinase produced by Aspergillus terreus CCT 4083 grown on sugar cane bagasse | en |
dc.type | Artigo | pt-BR |
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