Navegando por Autor "Kiyasov, Andrey P."
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Item Human adipose-derived stem cells stimulate neuroregeneration(Clinical and Experimental Medicine, 2005-06-06) Lima, Luciana M.; Masgutov, Ruslan F.; Masgutova, Galina A.; Zhuravleva, Margarita N.; Salafutdinov, Ilnur I.; Mukhametshina, Regina T.; Mukhamedshina, Yana O.; Reis, Helton J.; Kiyasov, Andrey P.; Palotás, András; Rizvanov, Albert A.Traumatic brain injuries and degenerative neurological disorders such as Alzheimer’s dementia, Parkinson’s disease, amyotrophic lateral sclerosis and many others are characterized by loss of brain cells and supporting structures. Restoring microanatomy and function using stem cells is a promising therapeutic approach. Among the many various sources, adipose-derived stem cells (ADSCs) are one of the most easily harvested alternatives, they multiply rapidly, and they demonstrate low immunogenicity with an ability to differentiate into several cell types. The objective of this study was to evaluate the effect of xenotransplanted human ADSCs on post-traumatic regeneration of rat sciatic nerve. Peripheral reconstruction following complete sciatic transection and autonerve grafting was complemented by intra-operative injection of hADSCs into the proximal and distal stumps. The injury caused gliosis and apoptosis of sensory neurons in the lumbar 5 (L5) ganglia in the control rodents; however, animals treated with hADSCs demonstrated a smaller amount of cellular loss. Formation of amputation neuroma, which hinders axonal repair, was less prominent in the experimental group, and immunohistochemical analysis of myelin basic protein showed good myelination 65 days after surgery. At this point, control groups still exhibited high levels of microglia/macrophage-specific marker Iba-1 and proliferating cell nuclear antigen, the mark of an ongoing inflammation and incomplete axonal growth 2 months after the injury. This report demonstrates that hADSCs promote neuronal survival in the spinal ganglion, fuel axonal repair and stimulate the regeneration of peripheral nerves.Item Transcriptional analysis of blood lymphocytes and skin fibroblasts, keratinocytes, and endothelial cells as a potential biomarker for alzheimer’s disease(Journal of Alzheimer's Disease, 2016-10-18) Lima, Luciana Moreira; Mukhamedyarov, Marat A.; Rizvanov, Albert A.; Yakupov, Eduard Z.; Zefirov, Andrey L.; Kiyasov, Andrey P.; Reis, Helton J.; Teixeira, Antônio L.; Vieira, Luciene B.; Salafutdinov, Ilnur I.; Petukhova, Elena O.; Khaiboullina, Svetlana F.; Schlauch, Karen A.; Lombardi, Vincent C.; Palotás, AndrásAlzheimer’s disease (AD) is a devastating and progressive form of dementia that is typically associated with a build-up of amyloid-β plaques and hyperphosphorylated and misfolded tau protein in the brain. Presently, there is no single test that confirms AD; therefore, a definitive diagnosis is only made after a comprehensive medical evaluation, which includes medical history, cognitive tests, and a neurological examination and/or brain imaging. Additionally, the protracted prodromal phase of the disease makes selection of control subjects for clinical trials challenging. In this study we have utilized a gene-expression array to screen blood and skin punch biopsy (fibroblasts, keratinocytes, and endothelial cells) for transcriptional differences that may lead to a greater understanding of AD as well as identify potential biomarkers. Our analysis identified 129 differentially expressed genes from blood of dementia cases when compared to healthy individuals, and four differentially expressed punch biopsy genes between AD subjects and controls. Additionally, we identified a set of genes in both tissue compartments that showed transcriptional variation in AD but were largely stable in controls. The translational products of these variable genes are involved in the maintenance of the Golgi structure, regulation of lipid metabolism, DNA repair, and chromatin remodeling. Our analysis potentially identifies specific genes in both tissue compartments that may ultimately lead to useful biomarkers and may provide new insight into the pathophysiology of AD.