Navegando por Autor "Kasuya, Maria C. M."
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Item Epulorhiza epiphytica sp. nov. isolated from mycorrhizal roots of epiphytic orchids in Brazil(Mycoscience, 2003-04) Matsuoka, Kiyoshi; Pereira, Olinto L.; Rollemberg, Christtianno L.; Borges, Arnaldo C.; Kasuya, Maria C. M.Epulorhiza epiphytica sp. nov. isolated from the roots of two Brazilian native epiphytic orchid species is described. In culture, it differs from the known species of Epulorhiza in the minute size of monilioid cells with foveate surfaces. This is the first report of an orchid mycorrhizal fungus from Brazil.Item In vivo bioavailability of selenium in enriched Pleurotus ostreatus mushrooms(Metallomics, 2010-01-07) Silva, Marliane C. S. da; Naozuka, Juliana; Oliveira, Pedro V.; Vanetti, Maria C. D.; Bazzolli, Denise M. S.; Costa, Neuza M. B.; Kasuya, Maria C. M.The in vivobioavailability of Se was investigated in enriched Pleurotus ostreatus mushrooms. A bioavailability study was performed using 64 Wistar male rats separated in 8 groups and fed with different diets: without Se, with mushrooms without Se, with enriched mushrooms containing 0.15, 0.30 or 0.45 mg kg^−1Se and a normal diet containing 0.15 mg kg^−1 of Se using sodium selenate. The experiment was performed in two periods: depletion (14 days) and repletion (21 days), according to the Association of Official Analytical Chemists. After five weeks, the rats were sacrificed under carbon dioxide, and blood was drawn by heart puncture. Blood plasma was separated by centrifugation. The total Se concentration in the plasma of rats fed with enriched mushrooms was higher than in rats fed with a normal diet containing sodium selenate. The plasma protein profiles were obtained using size exclusion chromatography (SEC) and UV detectors. Aliquots of effluents (0.5 mL per minute) were collected throughout in the end of the chomatographic column. However, Se was determined by graphite furnace atomic absorption spectrometry (GF AAS) only in the aliquots where proteins were detected by SEC-UV. The plasma protein profile of rats fed with different diets was similar. The highest Se concentration was observed in a peptide presenting 8 kDa. Furthermore, the higher Se concentration in this peptide was obtained for rats fed with a diet using enriched mushrooms (7 μg L^−1Se) compared to other diets (2–5 μg L^−1Se). These results showed that Se-enriched mushrooms can be considered as an alternative Se food source for humans, due to their high bioavailability.Item Small heat shock proteins in the development of thermotolerance in Pisolithus sp.(Journal of Thermal Biology, 2005-12) Ferreira, Adão S.; Kasuya, Maria C. M.; Araújo, Elza F.; Borges, Arnaldo C.; Tótola, Marco. R.Small heat shock proteins (HSPs) have been shown to confer thermotolerance in many organisms. Here, we demonstrate that small HSPs (sHSPs) can also be involved in development of thermotolerance in Pisolithus sp. In heat shock response, Pisolithus isolate RV82 synthesized proteins of molecular mass 28, 26 and 15–18 kDa. These group of proteins are synthesized when mycelial mass are exposed to heat shock temperature (42 °C) for short period (30 min) and incubated back at 28 °C, the optimal temperature for growth. Our results show sHSPs are an important biochemical alteration in ectomycorrhizal fungi under thermal stress.