Navegando por Autor "Dias, Júlio César Oliveira"
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Item Association of vitamin e with rapid thawing on goat semen(The Scientific World Journal, 2014-05-11) Penitente-Filho, Jurandy Mauro; Oliveira, Fabrício Albani; Jimenez, Carolina Rodriguez; Carrascal, Erly; Dias, Júlio César Oliveira; Oliveira, Gisele Dias; Silveira, Renata Gomes; Silveira, Camila Oliveira; Torres, Ciro Alexandre AlvesThe aim of this study was to evaluate the effects of vitamin E associated with rapid thawing on cryopreserved goat semen. Two bucks were used and eight ejaculates per animal were collected using artificial vagina. Semen was diluted with the following treatments: BIOXCELL (control), BIOXCELL + Equex (sodium lauryl sulphate) and BIOXCELL + vitamin E 100 μM. Semen was packaged into 0.25 mL straws and cooled at 5 ∘ C for 1 hour. Freezing was performed in liquid nitrogen vapor (−155 ∘ C) during 15 minutes. Then, the straws were immersed in liquid nitrogen (−196 ∘ C). Straws were thawed at 38 ∘ C/60 seconds or at 60 ∘ C/7 seconds with immediate sperm analysis. Hypoosmotic swelling test was performed adding a 20 μL aliquot of thawed semen to 1 mL of hypoosmotic solution (100 mOsm⋅Kg −1 ) followed by incubation during 60 minutes in water bath (38 ∘ C). Vitamin E did not affect any studied parameters (P > 0.05). Nevertheless, defrosting rate of 60 ∘ C/7 seconds improved sperm membrane functional integrity (P < 0.05). Current knowledge about goat semen cryopreservation is not sufficient to ensure high post-thawing recovery rates; thus, this study brings important data about using antioxidants and different thawing rates on cryopreservation process.Item Seasonal variation in the reproductive activity of male goats raised under tropical climate conditions(Revista Brasileira de Zootecnia, 2016-10-19) Dias, Júlio César Oliveira; Veloso, Cristina Mattos; Santos, Madriano Christilis da Rocha; Oliveira, Carlos Thiago Silveira Alvim Mendes de; Silveira, Camila Oliveira; Iglesias, Erick; Maitan, Paula Piccolo; Sanglard, Letícia Maria PereiraThis study analyzed seasonal variations in the testes, the concentration of sex hormones, the parameters of fresh and thawed semen, and the sexual behavior of male Alpine goats from a temperate region in a tropical climate and possible interference with fertility. The maximum and minimum temperature and luminosity were recorded daily, while seminal, hormonal, and behavioral assessments were carried out every fortnight. The maximum and minimum temperature (°C) and luminosity (h) were recorded daily always at 17.00 h. The scrotal circumference (cm), testicular volume (mL), volume (mL), appearance (creamy, milky, aqueous) and coloration (white, white-yellowish, and yellowish) seminal, turbulence or mass movement (0 to 5), progressive spermatic motility (0 to 100%), spermatic force (0 to 5), concentration (spermatozoids/mL), spermatic pathologies, hypoosmotic test (%), serum levels of FSH (mUI/mL), LH (mUI/mL), testosterone (ng/mL), and sexual behaviors were carried out every fortnight. There was a difference between the scrotal circumference evaluated monthly, testicular volume, volume and concentration of fresh semen, sperm vigor of the thawed semen, serum levels of testosterone, FSH and LH, and some sexual behaviors. Thus, the changes that occur in the quantity and quality of sperm, in the hormonal profile, and in sexual behaviors should not be regarded as an impediment to the use of male Alpine goats in tropical climates throughout the year. These variations do not lead to changes in the semen that may compromise the fertility of these animals.