Navegando por Autor "Cregan, Perry B."
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Item Linkage fine-mapping and QTLs affecting morpho-agronomic traits of a Mesoamerican × Andean RIL common bean population(Euphytica, 2018-12) Silva, Leonardo Corrêa da; Cruz, Cosme Damião; Carneiro, Pedro Crescêncio Souza; Silva, Fabyano Fonseca e; Barros, Everaldo Gonçalves de; Vianello, Rosana Pereira; Fonseca, Carlos Eduardo Lazarini da; Cregan, Perry B.; Song, Qijian; Carneiro, José Eustáquio de Souza; Souza, Thiago Lívio Pessoa Oliveira deThis paper proposes the construction of a genetic linkage map with 376 recombinant inbred lines (RILs) derived from a cross between Mesoamerican × Andean common bean (Phaseolus vulgaris L) parents based on single nucleotide polymorphism (SNP) markers; and to detect quantitative trait loci (QTLs) associated with seven morpho-agronomic traits: number of days to flowering (DF), number of days to maturity (DM) or crop cycle; plant architecture (ARC); seed yield (YLD); degree of seed flatness (SF); seed shape (SS); and 100-seed weight (SW). A total of 3060 polymorphic SNP markers were used and 2041 segregated at a 1:1 ratio in the RIL population, as expected. These markers were subjected to linkage analysis in each chromosome. The genetic linkage analysis resulted in linkage maps with a total of 1962 markers spanning 1079.21 cM. A total of 29 QTLs associated with seven morpho-agronomic traits were detected on the 11 chromosomes, which explained between 3.83 and 32.92% of the phenotypic variation in DF. A total of 18 candidate genes associated with the detected QTLs were identified and related with biological processes, molecular functions and cellular components.Item Single nucleotide polymorphism discovery in common bean(Molecular Breeding, 2011-09-13) Souza, Thiago Lı́vio P. O.; Barros, Everaldo G. de; Bellato, Claudia M.; Hwang, Eun-Young; Cregan, Perry B.; Pastor-Corrales, Marcial A.Single nucleotide polymorphisms (SNPs) were discovered in common bean (Phaseolus vulgaris L.) via resequencing of sequence-tagged sites (STSs) developed by PCR primers previously designed to soybean shotgun and bacterial artificial chromosome (BAC) end sequences, and by primers designed to common bean genes and microsatellite flanking regions. DNA fragments harboring SNPs were identified in single amplicons from six contrasting P. vulgaris genotypes of the Andean (Jalo EEP 558, G 19833, and AND 277) and Mesoamerican (BAT 93, DOR 364, and Rudá) gene pools. These genotypes are the parents of three common bean recombinant inbred line mapping populations. From an initial set of 1,880 PCR primer pairs tested, 265 robust STSs were obtained, which could be sequenced in each one of the six common bean genotypes. In the resulting 131,120 bp of aligned sequence, a total of 677 SNPs were identified, including 555 single-base changes (295 transitions and 260 transversions) and 122 small nucleotide insertions/deletions (indels). The frequency of SNPs was 5.16 SNPs/kb and the mean nucleotide diversity, expressed as Halushka’s theta, was 0.00226. This work represents one of the first efforts aimed at detecting SNPs in P. vulgaris. The SNPs identified should be an important resource for common bean geneticists and breeders for quantitative trait locus discovery, marker-assisted selection, and map-based cloning. These SNPS will be also useful for diversity analysis and microsynteny studies among legume species.