Navegando por Autor "Campos, Wellington G."
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Item Does mechanical damage on soybean induces the production of flavonoids?(Anais da Academia Brasileira de Ciências, 2018-10) Silva, Paulo Luiz da; Cordeiro, Gláucia; Silva, Carolina R. da; Barros, Rafael A.; Silva, Camila R. da; Zanuncio, José C.; Campos, Wellington G.; Oliveira, Maria G. A.The response of plants to grazing includes the production of chemical defense compounds such as proteases inhibitors and secondary metabolites as flavonoids, which makes them less palatable to feeding and negatively affecting the physiology of insects. The aim of this study was to evaluate the phytochemical response of soybean cultivars (Glycine max (L.) Merrill) resistant (IAC-17, IAC-24) and susceptible (IAC-P1) to insects after mechanical damage. These cultivars were mechanically injured, and after 24 hours samples of these plants were analyzed by HPLC to identify and quantify flavonoids. The flavonoids daidzein, quercetin, and rutin were quantified, with the highest concentration of daidzin in soybean cultivars after mechanical damage. Rutin was biosynthesized by IAC-24. The cultivars IAC-PL1, IAC-17, and IAC- 24 did not show a flavonoid response to mechanical damage. The soybean cultivars are not dependent on mechanical damage to produce flavonoids.Item Protease inhibitory, insecticidal and deterrent effects of the trypsin- inhibitor benzamidine on the velvetbean caterpillar in soybean(Anais da Academia Brasileira de Ciências, 2018-12) Silva, Carolina R.; Pilon, Anderson M.; Campos, Wellington G.; Cordeiro, Glaúcia; Silva, Camila R.; Oliveira, Maria Goreti A.The recognition of protease inhibitors with insecticidal activity is important as a basis for the development of mimetic peptides with potential use as biorational insecticides. We sprayed benzamidine on soybean plants and assessed whether this potent synthetic trypsin-inhibitor has protease inhibitory, insecticidal and deterrent effects on the velvetbean caterpillar Anticarsia gemmatalis Hübner (Lepidoptera: Erebidae). Activity of trypsin inhibition in soybean leaves was increased and total proteolytic activity in the midgut extract from larvae fed on these leaves was reduced by benzamidine. Different concentrations of benzamidine sprayed on the plant caused approximately 50 % of larval mortality, and larval choice and moth preference and oviposition were all negatively affected. Low concentrations of benzamidine increased mortality and hindered insect choice and oviposition as well as higher doses. Since many synthetic protease inhibitors are usually expensive, small doses of benzamidine may be effective to protect soybean against A. gemmatalis attack. Our results highlight the potential of synthetic protease inhibitors for insecticidal and deterrent purposes in insect pest management.