Reproducibility of the RAPD marker and its efficiency in coffee tree genotype grouping analysis
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Crop Breeding and Applied Biotechnology
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The genetic diversity of Coffea arabic L. cultivars is relatively narrow and its assessment and increase isimportant for breeding. Fifty two arbitrary primerswere used to evaluate the reproducibilityand the influence ofthe number of RAPD (Random Amplified Polymorphic DNA) markers on the estimation of genetic distancesamong 40 genotypes of Coffea spp. The average number of polymorphic bands was 6.69 per primer among allgenotypes, and 1.27 among arabica coffee genotypes. RAPD markers were efficient in estimating the geneticdistances among the genotypes. The increase in RAPD loci number during grouping analysis did not affect themajor groups’ composition; however, it affected the composition of subgroups. Marker reproducibility was76.88% and replicated data was recommended for distinguishing genotypes with the same genetic background.
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CABRAL, Terezinha Aparecida Teixeira; SAKIYAMA, Ney Sussumu; ZAMBOLIM, Laércio; PEREIRA, Antonio Alves; BARROS, Everaldo Gonçalves; SAKIYAMA, Cássia Camargo Harger. Reproducibility of the RAPD marker and its efficiency in coffeetree genotype grouping analysis. Revista Crop Breeding and Applied Biotechnology, Viçosa, v. 2, n. 1, p. 121-130, 2002.